ABSTRACT
Cancers evolution is a process of clonal selection,cell competition and clonal expansion. Cancers evolution includes cancer memory,heterogeneity,resistance,transformation and adaption et al.This evolution enables cancer cells to adapt to any hostile environment and to survive.An understanding of cancers evolution will provide a theoretical guidance for the implementation of the precision therapy.
ABSTRACT
La clonación posee dos finalidades bien definidas: la reproducción del organismo mediante la duplicación del genoma y la finalidad terapéutica, que incluye la clonación de órganos y tejidos para transplantar órganos y sustituir cadenas de genes anormales por otros sin anomalías. Esta revisión bibliográfica tiene como objetivo explicar de forma didáctica las técnicas de este proceder conocidas en el ámbito científico, de manera que puedan ser usadas como herramientas del profesional cubano en sus pesquisas diarias y dilucidar cómo la bioética enfoca la clonación reproductiva de forma negativa o ilícita, por las implicaciones sociales y morales que presupone. La clonación terapéutica es considerada importante e interesante desde el punto de vista científico y ético, ya que puede conducir a resolver varios problemas de salud.
Cloning possesses two well definite purposes: the reproduction of the organism by means of the duplication of the genome and the therapeutic purpose that includes the cloning of organs and tissues to transplant organs and to substitute chains of abnormal genes by other without anomalies. This bibliographical revision has as an objective, to explain in a didactic form the techniques of this proceed known in the scientific field, so these can be used as tools of cuban professionals in its daily searches and to clarify how bioethics focuses the reproductive cloning of negative or illicit form, by the moral and social implications that assumes. The therapeutic cloning is considered important and interesting from the ethical and scientific points of view, since it may solve several health problems.
ABSTRACT
La biotecnología, aunque tan antigua como la invención del vino y la fermentación de la cerveza por el hombre, tiene en los años 60 del siglo XX, un importante despegue con las técnicas de biología molecular y clonaje de genes foráneos en organismos unicelulares, y el surgimiento de los primeros medicamentos recombinantes. En la década de 1980 y 1990, se obtiene sorprendentes resultados de transgénesis de animales y plantas y ya finalizando el siglo aparece la clonación ó generación de animales idénticos a partir de células somáticas. En este trabajo se valora críticamente las oportunidades y riesgos de la transgénesis y la clonación en el plano ético actual, teniendo en cuenta la amenaza de los seres clónicos, la responsabilidad social de los científicos, la polarización del sistema científico internacional y los códigos morales, además de la ética del profesional científico cubano que responde al principio de que el futuro de nuestra patria tiene que ser necesariamente un futuro de hombres de ciencia.
Even though Biotechnology is as old as the invention of wine and beer fermentation by men, it experiences an important take off in the 60s, with the development of molecular biology techniques and cloning of strange genes in unicellular organisms, and the emergence of the first recombinant medications. In the decade of 1980 and 1990, surprising results in transgenesis of animals and plants are obtained and by the end of the century cloning or generation of identical animals starting from somatic cells is carried out. In this work the opportunities and risks of transgenesis and cloning are analyzed critically taking into account the current ethical perspective, keeping in mind the threat of cloned beings, the social responsibility of scientists, the polarization of the international scientific system and moral codes, as well as the Cuban science professionals ' ethics that responds to the principle that the future of our homeland is necessarily a future of science men.
ABSTRACT
Objective To screen the Doc 1R gene recombinant plasmid by use of colony PCR. Method The recombinant colonies were transfered into the PCR reaction mixture. The PCR primers were used for constructing mouse Doc 1R genomic sequence. Result Among the 5, 3 positive strips in the size of 1 500 bp were visible, which were the same as the Doc 1R gene fractions in terms of their sizes were screened as positive clones. The positive colony were further confirmed by double digestion and DNA sequencing. Conclusion Colony PCR is a simple, efficient and reliable technique for screening the recombinant.
ABSTRACT
To clone the full length cDNA sequence of a novel expression sequence tag ST55 (GenBank Accession No. BI121646) from human umbilical vein endothelial cells stimulated by lipopolysaccharide, rapid amplification of cDNA ends (RACE) was used to extend the 3′and 5′ends of ST55 to obtain the full length cDNA sequence according to the known ST55 sequence. The cDNA was identified by Northern blot and analyzed with bioinformatics. A novel human full length cDNA was cloned and named endothelial overexpressed lipopolysaccharide associated factor 1( EOLA1 ) (GenBank Accession No. AY074889). This gene was located at chromosomal Xq27.3 and encodes a protein EOLA1 composed of 158 amino acids. Our data show that EOLA1 is a novel human gene associated with activated endothelial cells, and may play a role in intercellular signal transduction.