Determination of aloin of compound aloe ointment by HPLC / 国际中医中药杂志

Objective To establish a method for the determination of aloin of compound aloe ointment. Methods HPLC method was used with Agilent Zorbax-C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-0.08%Sodium 1-heptanesulfonate (22∶78). The flow rate was 1.0 ml/min,the column temperature was 30 ℃, and the UV detector was set at 355 nm. Results The linear ranges of aloin was 0.037~0.74 mg/ml(r=0.999 8). The average recovery of aloin was 98.7%. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of aloin in compound aloe ointment.