ABSTRACT
Objective To develop the methods for identification and determination of scutellarin in compound Granule Tetrandra .Methods A TLC method was used to identify quality for scutellarin ,and a HPLC method was used to determine the content of scutellarin .Results The spot was clear in TLC identification without interference of negative control .The sam-ple size of scutellarin had a good linear relationship with peak area r=0 .9996(n=5)when ranged from 22 .4~156 .8 μg/ml , with the average recovery rate 97 .79% ( RSD=0 .32% ,n=6) .Conclusion The method is simple ,with good specificity and reproducibility ,which can be used as the quality control for this preparation .
ABSTRACT
Objective: To study the content difference of berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, and paeoniflorin in Wujiwan traditional decoction, granule prescription, and compound granule using HPCE internal standard method. Methods: Capillary zone electrophoresis (CZE) method was used for a complete separation of the components, which was achieved with 50 mmol/L borax buffer-methanol (2:1), constant voltage of 25 kV, pressure injection 5 kPa × 15 s, electrolyle sealing 5 kPa × 10 s, and column temperature of 15°C. Results: The capillary electrophoresis method is simple and accurate. The contents of the three alkaloids in the granule prescription were obviously higher than those in traditional decoction and compound granule. Conclusion: This method is instructive for clinical application and quality control of Wujiwan prescription and compound granule.