ABSTRACT
Aim: The aim of this study was to evaluate the effect of Morinda citrifolia juice (MCJ) and Triphala on sealer penetration depth into the dentinal tubules as root canal irrigants. Materials and Methods: Forty five single rooted extracted human teeth were collected; decoronated and root canal length were standardized to16mm. Cleaning and shaping was done with protaper universal rotary instruments till F5. 30 samples were selected randomly and divided into two groups with 15 samples in each, according to the final irrigation regimen. In Group I, 5 ml of MCJ was used for 1min and in Group II; 5 ml of Triphala was used. The remaining 15 samples were irrigated with 5ml of Smear clear which acted as a positive control Group. Obturation was done with corresponding gutta-percha points and AH 26 sealer (Dentsply; DeTrey, Konstanz, Germany) labelled with fluorescent dye and left to set for 48 hrs. Then, the roots were sectioned and subjected to confocal laser microscope. Results: Statistical analysis was done by two-way analysis of variance (ANOVA) and Tukey multiple post-hoc procedures. The sealer penetration depth is higher in the MCJ group than the Triphala group at all three levels. Conclusion: MCJ showed a significant sealer penetration depth than Triphala.
ABSTRACT
Introducción: El Microscopio Láser Confocal de Reflectancia (MCR) es una técnica no invasiva, con resolución cuasi histológica, que permite la visualización de células y estructuras de la piel. Esta tecnología es especialmente útil en el diagnóstico de lesiones tumorales. Material y métodos: Se trata de un estudio retrospectivo que busca medir la sensibilidad y especificidad del MCR en el diagnóstico de lesiones tumorales de la piel. Luego de obtener imágenes con el MCR de las lesiones, éstas se resecaron y se enviaron a estudio histopatológico, observando la correlación entre ambas técnicas. Resultados: Encontramos un 98 por ciento de concordancia entre el diagnóstico por MCR y el diagnóstico histopatológico para el total de tumores. El MCR mostró una sensibilidad y especificidad de 100 por ciento paramelanomas y sensibilidad de 93 por ciento y especificidad de 100 por ciento para carcinoma basocelular. En el caso de nevus atípico, la sensibilidad fue de 100 por ciento y la especificidad de 97 por ciento. Sólo dos lesiones que en la histopatología demostraron ser carcinomas basocelulares superficial y nodular fueron confundidas conqueratosis liquenoide y nevus atípico respectivamente. Discusión: Este estudio demostró una alta concordancia entre el MCR y la histopatología en tumores malignos. Sin duda que el MCR es y seguirá siendo un pilar fundamental en el diagnóstico de las lesiones tumorales de la piel, así como en la investigación y seguimiento in vivo de diversas lesiones de la piel.
Introduction: The Reflectance Confocal Laser Microscope (RCLM) is a new non-invasive technology, with quasi-histological resolution, which allows visualization of cells and skin structures and is particularly useful in the diagnosis of skin tumors. Material and methods: This is a retrospective study aimed to determine the diagnostic sensitivity and specificity of the RCLM in tumors of the skin using conventional histology as the gold standard for diagnosis. The images were obtained from patients with tumors of the skin using the RCLM, which were subsequently excised and sent to histopathological analysis. Results: We found a 98 percent concordance between the previous diagnosis by RCLM and pathology results. Diagnosis by confocal microscopy showed a sensitivity and specificity of 100 percent for melanomas, 93 percent sensitivity and 100 percent specificity for basal cell carcinoma and a sensitivity of 100 percent and specificity of 97 percent for atypical nevi. Only 2 lesions on histopathology proved to be superficial and nodular basal cell carcinomas were confused with lichenoid keratosis and atypical nevi, respectively. Discussion: We believe that RCLM is and will remain a really useful tool in the diagnosis of tumours of the skin as well as in research and in vivo monitoring of various types of skin lesion.