ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid, baicalin and imperatorin in Biyanling tablets. METHODS:HPLC method was adopted. The separation was performed on a Waters SunFire C18 column with mobile phase consisted of methanol-0.20% phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and column temperature was 30 ℃. The sample size was 20 μ L. RESULTS:Linear ranges of chlorogenic acid,baicalin and imperatorin were 1.618-16.18 μg/mL,1.624-16.24 μg/mL and 1.762-17.62 μg/mL(r were 0.999 9),respectively. The limits of quantitation were 2.67,2.03,1.87 μg/mL,and the limits of detection were 1.34,1.06, 0.98 μ g/mL,respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 98.01%-101.80%(RSD＝1.38%,n＝9),98.38%-101.40%(RSD＝1.26%,n＝9)and 98.06%-101.40%(RSD＝1.28%,n＝9), respectively. RSDs of tolerance tests were all lower than 2.0%. CONCLUSIONS:The method is simple,accurate,precise,stable, reproducible and durable. It can be used for simultaneous determination of hlorogenic acid,baicalin and imperatorin in Biyanling tablets.