Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Hemin can improve the stress resistance of plants through the heme oxygenase system. Additionally, substances contained in plants, such as secondary metabolites, can improve stress resistance. However, few studies have explored the effects of hemin on secondary metabolite content. Therefore, the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in

Research progress on Conyza blinii / 中草药

Conyza blinii is a genuine herb distributed in the Sichuan, Yunnan, and Guizhou provinces of China. It has effects of clearing heat, removing toxin, and relieving cough and asthma. It is mainly used for the treatment of cough and other inflammatory diseases caused by bronchitis. In this review, the relevant researches about resource distribution, chemical composition, pharmacological effects, genetic diversity, secondary metabolites synthesis pathways, and applications of C. blinii were collected and summarized. This work aims to provide a reference for the development and utilization of this medicinal herb and new drug products.

Cloning and sequence analysis of glyceraldehyde-3-phosphate dehyrogenase gene from Conyza blinii / 中草药

Objective: To clone and analyze glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from Conyza blinii and detect whether it can be the reference gene for C. blinii. Methods: Full length GAPDH was cloned by RACE. DNAMAN, BLAST, and MEGA bioinformatic tools were used to analyze its open reading frame (ORF), homology, and phylogenetic tree. The sequence was acted as internal control gene for semi-quantitative RT-PCR. Results: The gene designated GLGAPDH was 1 418 bp in length. It contained a 1 020 bp ORF encoding 340 amino acids. It shared 96% similarity with Gynura bicolor GAPDH and shared 93% similarity with Mikania micrantha GAPDH. GLGAPDH had closer relationship with GAPDH in G. bicolor. When the sequence acted as internal control gene, the semi-quantitative RT-PCR had benign amplification and good reproducibility. Conclusion: GAPDH is cloned from C. blinii for the first time. The semi-quantitative RT-PCR results prove that GAPDH gene is able to be the reference gene for gene expression analysis. The result of this study will provide the basis for the key enzyme expression and regulate the mechanism analysis in C. blinii effective components biosynthesis pathway.