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【Objective】 To explore the research status, hot spots and trends of platelet rich plasma (PRP). 【Methods】 With "platelet rich plasma (PRP)" and its Chinese equivalent as the subject words, the PRP related articles during January 1, 2013 to December 31, 2022 from PubMed and CNKI database were retrieved. The bibliometric analysis was performed by Bicomb 2.0 software to extract the annual number of literature publications, authors, journals and high-frequency theme words/sub theme words. The gCLUTO software was used to evaluate and visualize the results, and strategic diagram was drawn according to the results of biclustering. 【Results】 A total of 9 066 PRP related articles were retrieved (7 027 from PubMed, 2 039 from CNKI), and the number of publications showed an increasing trend year by year. Papers have been published in 1 527 journals in PubMed, among which the journal with the highest number of publications was Arthroscopy: Journal of Arthroscopic & Related Surgery (175 articles), followed by American Journal of Sports Medicine (171 articles ) and Journal of Cosmetic Dermatology (121 articles) . PRP-related studies were published in 541 journals in CNKI, with the top 3 journals as Chinese Tissue Engineering Research (113 articles), Chinese Aesthetic Medicine (64 articles) and Chinese Journal of Blood Transfasion (45 articles) . In Pubmed, Anitua Eduardo (84 articles), Filardo Giuseppe (53 articles) and Cole Brian J (44 articles) were the top three productive authors on PRP; Cheng Biao from the General Hospital of Southern Theater Command of Chinese PLA was the most productive Chinese author (25 articles) . Shan Guiqiu from the General Hospital of the Southern Theater Command of the Chinese People's Liberation Army published the most articles(29 articles) in CNKI. American journals published the most articles (2 745 articles ), accounting for 39.06% of the total articles, followed by British and Swiss journals, with 1 499 articles and 550 articles, respectively. A total of 42 high-frequency subject words/sub-subject words were selected from PubMed, and were classified into 6 roups, while 30 high-frequency subject words were selected from CNKI and grouped into 5 categories. The strategic coordinates show that the treatment of rotator cuff and tendon injury with PRP in PubMed, the study of PRP and tissue engineering materials in CNKI are the core themes of current PRP research. 【Conclusion】 The strategic coordinate map and bibliometrics can reveal the current research status of PRP and predict future research hotspots, but current research cores of PubMed and CNKI are not consistent, and further research is still needed.
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ObjectiveTo analyze the online public Q&A texts on HPV vaccine， focus on the important issues related to HPV vaccination and cervical cancer prevention in China， and propose strategies and suggestions. MethodsThe latent Dirichlet allocation （LDA） topic model was employed to extract key topics of 15 565 Q&A texts related to HPV vaccines from the social Q&A platform "Zhihu". The Baidu AI sentiment analysis tool was used to analyze the emotional tendencies of the texts corresponding to each topic， and the topics were classified based on the strategic coordinate method. ResultsOnline users focused on eight topics about HPV vaccine information. Among them， vaccination knowledge， HPV vaccination hesitation， and HPV vaccine development and marketing belonged to the low positivity-high negativity emotional topics， HPV infection and high-risk factors belonged to the low positivity-low negativity emotional topics， and HPV vaccine appointment channels， comparison between domestic and imported vaccines， HPV vaccines and cervical cancer prevention， and HPV vaccine types and selection were grouped under high positivity-low negativity emotional topics. ConclusionPublic concerns regarding HPV vaccines can be classified into three major dimensions： health knowledge， health beliefs， and health behaviors. Overall， the public's views and attitudes towards vaccine-related issues are not optimistic. Strengthening science publicity and education， enhancing vaccine supervision， and encouraging enterprises’ innovative research and development capability are effective strategies to improve public awareness of cervical cancer prevention and accelerate the full HPV vaccination coverage.
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Resumen: La técnica anestésica neuroaxial en la paciente embarazada obesa clase III puede representar un reto para el anestesiólogo, debido a la dificultad para reconocer las referencias anatómicas. El objetivo del estudio fue determinar la dificultad y la calidad de la anestesia combinada espinal/epidural, en 14 pacientes embarazadas con índice de masa corporal ≥ 40 kg/m2, programadas para cesárea. Para la localización del sitio de punción, se utilizó la autoidentificación de cintura, metodología no reportada previamente. Las pacientes se sentaron a horcajadas en la mesa quirúrgica, señalaron el lugar de su cintura con sus manos. Se trazaron dos rectas en la espalda, una recta horizontal en la cintura señalada por la paciente, y una recta vertical correspondiente a la línea media. La intersección de las dos rectas fue la coordenada para la punción neuroaxial. La media ± DE del índice de masa corporal fue 47.5 ± 3.7 kg/m2. Se determinó una tasa de éxito del primer nivel del 92.8%, un promedio de punciones de 1.5 y 57.1% de las pacientes recibió una punción. La anestesia espinal/epidural falló en dos casos, se realizó cambio de técnica anestésica a epidural, y complementación con infiltración local, con adecuada analgesia. No fue necesario convertir a anestesia general en ningún caso. La autoidentificación de la cintura puede ser una ayuda clínica para la identificación del sitio de la punción lumbar durante la instalación de la anestesia neuroaxial, en pacientes obstétricas con obesidad clase III programadas para cesárea.
Abstract: The neuraxial anesthetic technique in the obese class III, pregnant patient, can represent a challenge for the anesthesiologist, due to the difficulty in recognizing the anatomical landmarks. The objective of the study was to determine the difficulty and quality of combined spinal/epidural anesthesia, in 14 pregnant patients with a body mass index ≥ 40 kg/m2 scheduled for cesarean section. For the location of the puncture site, self-identification of the waist was used, a methodology that has not been previously reported. The patients straddled the surgical Table, pointing to her waist with both hands. Two lines were drawn on the back. A horizontal line at the waist, indicated by the patient, and a vertical line corresponding to the midline. The intersection of the two lines was the coordinate for the neuraxial puncture. The mean ± SD of the body mass index was 47.5 ± 3.7 kg/m2. A first level success rate of 92.8% was determined, 57.1% of the patients received one puncture, and the average of punctures was 1.5. Spinal/epidural anesthesia failed in two cases, a change from anesthetic technique to epidural and supplementation with infiltration was performed, with adequate analgesia. It was not necessary to convert to general anesthesia in any case. Self-identification of the waist and coordinate marking can be a clinical aid for the identification of the lumbar puncture site, during the installation of neuraxial anesthesia, in obstetric patients with class III obesity, scheduled for cesarean section.
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Objective:To explore the genetic diversity and population structure of <italic>Erigeron breviscapus</italic>， so as to provide a scientific basis for its resource protection and rational utilization. Method:Twelve pairs of simple sequence repeat（SSR） primers were screened out from 243 individuals in 16 natural populations to calculate the genetic diversity parameters of <italic>E. breviscapus</italic>， which were then subjected to principal coordinate analysis and cluster analysis. Result:Twelve SSR markers generated 209 alleles， with an average of 17.417 alleles per locus. Based on 12 SSR markers and 16 populations of <italic>E. breviscapus</italic>， the observed heterozygosity （<italic>H</italic><sub>0</sub>） values were determined to be 0.603 and 0.613， the expected heterozygosity （<italic>H</italic><sub>e</sub>）to be 0.658 and 0.659， and the Shannon's information index （<italic>I</italic>） to be 1.443 and 1.446， respectively. The Wright's fixation index （<italic>F</italic><sub>st</sub>） was 0.123 and gene flow （<italic>N</italic><sub>m</sub>） was 2.077. Analysis of molecular variance （AMOVA） and genetic differentiation revealed that genetic variation within populations was the main source of total variation. The Nei's genetic distance and genetic identity coefficients were within the ranges of 0.107 （YA and XY）-0.713 （SZ and XZD） and 0.490 （SZ and XZD）-0.899 （YA and XY）， respectively. As demonstrated by the principal coordinate analysis and cluster analysis， the 16 populations of <italic>breviscapus </italic>were divided into two clusters. Conclusion:The genetic diversity of <italic>E. breviscapus</italic> was relatively high and there existed certain genetic differentiation and gene flow within and among populations. The genetic variation was mainly present within populations. All these have provided reference for subsequent study on good germplasm selection of <italic>E. breviscapus.</italic>
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Aim: The present study was undertaken to examine the relation between genetic divergence of some tropical inbred lines of maize and extent of heterosis manifestation in their single cross hybrids. Methodology: Fifteen inbred lines and three inbred testers along with 45 single cross hybrids were evaluated by adopting randomized complete block design during two seasons. The observations recorded on eight metric traits of the entries in each of three replications were utilized during statistical analysis across the seasons. Relative importance of classification variables was determined by principal component analysis and the phenogram was generated using average taxonomic distance. Molecular profiling of inbred lines was carried out by employing 28 SSR primer pairs covering all the chromosomes. Principal coordinate analysis was performed and similarity indices based dendrogram was constructed. Relationship between parental genetic divergence and heterosis was statistically tested. Results: Significant differences due to parents, hybrids and parent vs. hybrids were observed for all metric characters. Principal component analysis based diagram and average taxonomic distance based phenogram separated the inbred lines into five clusters. Amplification profiles based principal coordinate analysis and hierarchical cluster analysis differentiated the inbred lines into four major clusters. Statistically non-significant correlation of genomic data based genetic similarity or phenotypic data based genetic dissimilarity of inbreds was revealed with grain yield and heterosis of hybrid combinations. Significantly positive correlation was found between heterosis over mid parent or better parent and grain yield of hybrids. Interpretation: Parental genetic divergence was not linearly or additively related to heterosis manifestation in hybrid. Moderate genetic divergence together with high per se performance of inbreds seemed to be a better criterion than consideration of merely extreme genetic diversity as the basis for the choice of parental inbreds during heterotic single cross hybrid development in maize
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Objective To explore the feasibility of our method on assessing the three-dimensional facial morphological features based on three-dimensional surface imaging techniques.Methods According to the admission criteria,three-dimensional facial images of 34 attractive young Chinese women and 172 healthy reference young women were selected from the three-dimensional facial images database of our department.For those images,anthropometric facial measurements including facial height,width,the degree of convexity and concavity were computed using three-dimensional surface imaging techniques combined with three-dimensional coordinate system.The variance between attractive group and reference group was analyzed and the facial morphological features of each group were preliminarily summarized.Results Both groups met the criteria of mesoprosopy,not the "vertical facial trisection and rule of fifths".The attractive group had a relatively narrow anterior facial frame,a more rounded and full upper face compared with the reference group.They also had smaller intercan thai width compared with the reference group.Moreover,the attractive group showed more prominent nose,more full and prominent medial cheek.In addition,the attractive group shared less protruded upper lips and less retruded chin compared with the reference group.Conclusions Assessing facial morphology using three-dimensional surface imaging techniques is a convenient and feasible method.The evaluation of sagittal facial convexity and concavity is an indispensable part of facial morphology features assessment.
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To solve the problem of precise positioning of carp brain tissue coordinates, it is proposed in this paper for a method for transforming the coordinates of magnetic resonance imaging of carp brain tissue into the coordinates of electrode implantation using a brain stereotaxic apparatus. In this study, the 3.0T magnetic resonance imaging instrument was used to scan the carp brain. We independently established the three-dimensional positioning coordinate system of the brain, the three-dimensional coordinate assistance system of skull surface and the three-dimensional coordinate assistance system in brain tissue. After two coordinate transformations, the magnetic resonance image coordinates of the brain electrodes implantation sites were converted into the three-dimensional stereotactic coordinate system to guide the electrodes implantation. The experimental groups were divided into two groups, A and B. Group A was the group of magnetic resonance imaging apparatus combining with the brain stereotaxic apparatus, and group B was the group of brain atlas combining with the brain stereotaxic apparatus. Each group had 20 tails of carps ( = 20). This two methods were used to implant the electrodes into the cerebellar motor area. The underwater experiments of the carp robots were carried out to test the two methods. The results showed that the accuracy of the implanted electrodes were 90% in group A and 60% in group B. The success rate of group A was significantly higher than that of group B ( < 0.05). Therefore, the new method in this paper can accurately determine the coordinates of carp brain tissue.
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Objective To investigate the feasibility of 3D-printing coplanar coordinate template (3D-PCT) for guiding 125I radioactive seed implantation in the treatment of pelvic wall recurrence of cervical cancer on ensuring the accuracy of dose.Methods From Oct 2016 to Dec 2017 in Peking University Third Hospital,totally 10 patients with pelvic wall recurrent cervical cancer after radiotherapy were treated with 125I radioactive seed implantation assisted by 3D-PCT.The median age was 53.5 years old (37-71 years old).KPS score of the cohort were more than 70.All patients had received pelvic radiation therapy previously.The median volume of the lesion was 31.9 cm3 (3.5-58.0 cm3).The prescription dose was 120-180 Gy.The activity of seeds was 0.55-0.67 mCi(1 Ci =3.7 × 1010Bq),while the number of seeds was 12-81 (median 50) on preoperative plan.Radioactive seeds implantation was performed under 3D-PCT guidance according to the preoperative plan.The actual number of implanted seeds was 53 (10-82).Dosimetry parameters of preoperative plan and postoperative plan including D90,D100,V100,V150,V200,external index (EI),conformal index (CI),heterogeneity index (HI),and organat-risk doses of D2 cm3,D1 cm3 and D0.1 cm3 were compared using the nonparametric test.Results The seed number of postoperative plan was more than that of preoperative plan (Z =-2.255,P ＜ 0.05),but all of the dosimetric parameters showed no significant difference (P ＞ 0.05).D2 cm3 and D1 cm3 of rectum for postoperative plan were lower than that of preoperative plan (Z =-2.100,-2.240,P ＜ 0.05),while other dose parameters of normal tisssues showed no statistically significant difference (P ＞ 0.05).Conclusions Assisted by 3D-PCT for 125I radioactive seed implantation in pelvic wall recurrence of cervical cancer,the actual postoperative dose could meet the requirement of the preoperatie plan through the intraoperative optimization of dose.3D-PCT could ensure the precise of delivered dose of 125I radioactive seed implantation.
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Objective To provide the scientific evidence for studying Alzheimer's disease (AD) and decision-making for its management by analyzing the strategic information of hotspots in studies on AD.Methods Citation strategic coordinate diagram was plotted by describing the history of studies on AD with co-citation clustering for further understanding the novelty and concern of hotspots in studies on AD.The current hotspots in studies on AD were discovered by co-words clustering and their strategic coordinates were analyzed to reveal their development periods.Results The current hotspots in studies on AD were substance metabolism in AD patients, etiology of AD and therapies for AD.The diagnosis of AD according to its clinical characteristics and hypothesis on amyloid cascade in AD patients were greatly concerned.Conclusion Citation strategic coordinate analysis and co-words strategic coordinate analysis can display the hotspots in studies on AD and their development history.
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Objective To study the mechanism of two-component system of MprAB and TrcRS in synergistically regulating gene rv1057 expression of Mycobacterium tuberculosis.Methods The in vivo specific binding capability of MprA and TrcR with the target gene promoter region was analyzed using electrophoretic mobility shift assay.The transcription level of target gene was analyzed by using fluorescence quantitative polymerase chain reaction,and all results were compared with the fold changes in H37Rv strain plus SDS group,which was set as one unit.The expression level of target gene was analyzed by using western blot;the transcription ability of different promoter region of rv1057 was detected through lacZ report gene.The t test was used for statistical analysis.Results MprA was able to bind to trcR promoter.The expressions of trcR in D981 and H37Rv strains without SDS were 1.7 and 2.5 folds of the expression of H37Rv strains with SDS groups,respectively.The difference between these two groups was statistically significant (t=18.54,P＜0.05).With SDS,the expressions of trcR in D981 and H37Rv strains were 1.0 and 2.1 folds of the expression of H37Rv strains plus SDS group,respectively.The expressions of trcR in D981 and H37Rv strains were significantly different (t=15.86,P＜0.05).After adding SDS during the culture of H37Rv strains,the expression of trcR in H37Rv decreased.The difference between these two groups was statistically significant (t=16.99,P＜0.05).Both MprA and TrcR were able to bind to rv1057 promoter and regulate its expression.MprA activated the expression of rv1057,while TrcR repressed the expression of rv1057.Conclusions MprAB and TrcRS synergistically regulate the expression of rv1057.MprA is activated in the presence of SDS,which represses the transcription of trcR and activates the transcription of rv1057.However,TrcR represses the transcription of rv1057 in the absence of SDS.
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Objective To analyze the published articles on the etiology of rheumatoid arthritis (RA) and catch the recent research hot topics.Methods Literature searching on the etiology of RA was conducted through PubMed database of US Congress Library of Medicine published in past five years.Bibliographic Item CO-Occurrence Matrix Builder (BICOMB) to bibliometric analysis and statistical product and service solutions (SPSS) 19.0 were used for cluster analysis and plotting strategic coordinate figure.Results Four research hot topics were identified by analyzing the keywords:① Therapeutic and adverse effects of antirheumatic agents;② Genetics and complications of RA;③ Immunity of autoantigens and autoantibodies in RA;④ Immune cells and microRNAs in RA.Conclusion Researches on RA etiology have developed steadily,with different current status and developing tendency,which provides reference for related studies.
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Objective According to the CT or MRI images of lumbar disc herniation (LDH),a simple and accurate coordinate partitioning method for describing the size and location of protrusions based on CT value was proposed.This method can provide a reference for the diagnosis and surgical treatment of LDH.Method The system was developed by experienced clinicians and imaging experts.In this system,CT and MR data of patients with LDH was analyzed,and classified by coordinate partition according to the degree of LDH.According to the lumbar disc anatomical and clinical features,the protrusions were located in X axis,Y axis,and Z axis.Results In this system,the position of the protrusions in the spinal canal was respectively partitioned from X axis (horizontal plane),Y axis (sagittal plane) and Z axis (coronal plane),where the X and Y axes were divided into 8 zones (-4 to 4) and the Z axis was divided into 4 zones (1 to 4).The size,i.e.the degree of protrusions,was described by the sum of the absolute values of the corresponding position zones.The greater the value,the more serious of the protrusion is.Conclusion The method of coordinate partitioning based on CT value can well reflect the pathological types,precise position and degree of LDH,and thus provide references for the diagnosis and the selection of surgical methods and surgical occasion of LDH.
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ABSTRACT Amorphophallus (elephant foot yam) is an herbaceous edible aroid crop which belongs to the family Araceae. The study was undertaken to identify the efficient SSR primer that could differentiate a set of 12 elephant foot yam genotypes. Various efficiency parameters, namely, Polymorphism Information Content (PIC), Marker Index (MI), Resolving Power (RP) and Diversity Index (DI) were studied for 11 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. Discrimination analysis was done to find out the most effective parameter. Finally Principal Coordinate Analysis (PCoA) and dendrogram was done to find out the genetic diversity among the germplasm. The SSR markers under this investigation will facilitate further studies in population genetics and utilization of A. paeoniifolius.
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The life cycle, evolution and trend of EEG technology were studied by plotting its S curve model ac-cording to its maturity theory with Derwent Innovation Index Database-covered EEG technology patent data as a sample. Cluster analysis and strategic coordinate analysis according to the co-classification network of Derwent pa-tent classification code showed the linkage relationship and evolution trend in different fields of EEG technology. The frequency and centrality of EEG technology were analyzed according to the co-word network of Derwent manual code in order to identify the distribution and hotspots of research on EEG technology.
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Radiotherapy is an important therapy for uterine cervical cancer and plays a great role in improving the outcome in uterine cervical cancer patients. The high frequency subject headings/subheadings in papers on radio-therapy for uterine cervical cancer in the past 10 years were analyzed by co-occurrence clustering analysis to dis-play the hot spots and a strategic coordinate figure was plotted to show the developing tendency of its hot spots in studies on radiotherapy for uterine cervical cancer in order to provide reference for its-related studies.
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The research hot spots and development in domestic neurology were analyzed by co-words analysis, visual analysis and the combination of them, which showed that the co-occurrence relation between high frequency words could display the structure and hot spots of subject headings in neurology, analysis of the cluster evolution and position could dynamically reveal the hot spots and development in neurology, and comprehensive analysis of words and their classes could directly and overall demonstrate the hot spots and development in neurology.
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The hand coordinate systems for measuring vibration exposures and biodynamic responses have been standardized, but they are not actually used in many studies. This contradicts the purpose of the standardization. The objectives of this study were to identify the major sources of this problem, and to help define or identify better coordinate systems for the standardization. This study systematically reviewed the principles and definition methods, and evaluated typical hand coordinate systems. This study confirms that, as accelerometers remain the major technology for vibration measurement, it is reasonable to standardize two types of coordinate systems: a tool-based basicentric (BC) system and an anatomically based biodynamic (BD) system. However, these coordinate systems are not well defined in the current standard. Definition of the standard BC system is confusing, and it can be interpreted differently; as a result, it has been inconsistently applied in various standards and studies. The standard hand BD system is defined using the orientation of the third metacarpal bone. It is neither convenient nor defined based on important biological or biodynamic features. This explains why it is rarely used in practice. To resolve these inconsistencies and deficiencies, we proposed a revised method for defining the realistic handle BC system and an alternative method for defining the hand BD system. A fingertip-based BD system for measuring the principal grip force is also proposed based on an important feature of the grip force confirmed in this study.
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Hand Strength , Hand , VibrationABSTRACT
Most of foot pain occurs by the entrapment of the tibial nerve and its branches. Some studies have reported the location of the tibial nerve; however, textbooks and researches have not described the posterior tibial artery and the relationship between the tibal nerve and the posterior tibial artery in detail. The purpose of this study was to analyze the location of neurovascular structures and bifurcations of the nerve and artery in the ankle region based on the anatomical landmarks. Ninety feet of embalmed human cadavers were examined. All measurements were evaluated based on a reference line. Neurovascular structures were classified based on the relationship between the tibial nerve and the posterior tibial artery. The bifurcation of arteries and nerves were expressed by X- and Y-coordinates. Based on the reference line, 9 measurements were examined. The most common type I (55.6%), was the posterior tibial artery located medial to the tibial nerve. Neurovascular structures were located less than 50% of the distance between M and C from M at the reference line. The bifurcation of the posterior tibial artery was 41% in X-coordinate, -38% in Y-coordinate, and that of the tibial nerve was 48%, and -10%, respectively. Thirteen measurements and classification showed statistically significant differences between both sexes (P<0.05). It is determined the average position of neurovascular structures in the human ankle region and recorded the differences between the sexes and amongst the populations. These results would be helpful for the diagnosis and treatment of foot pain.
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Humans , Ankle , Ankle Joint , Arteries , Cadaver , Classification , Diagnosis , Foot , Tibial Arteries , Tibial NerveABSTRACT
Objective: To analyze the genetic diversity of Panax japonicus var. major by inter simple sequence repeat (ISSR) molecular makers. Methods: Genetic diversity of 19 samples from main production areas was investigated by ISSR and analyzed by principal coordinate analysis and UPGMA cluster analysis. Results: A total of 181 bands were generated by 13 ISSR primers, among which 166 bands (91.71%) were polymorphic bands (PPB). The coefficient of genetic similarity ranged from 0.60 to 0.83. The results of UPGMA cluster analysis and principal coordinate analysis showed that the genetic diversity of P. japonicus var. major from the same region presented a geographical distribution regularity. Conclusion: The results of ISSR analysis reveals that P. japonicus var. major has a high genetic diversity level and the genetic relationship closely contacts with the geographical location.
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Objective To investigate the development and application of thoracic-abdominal tumor precise positioning puncture devices.Methods Based on the principle of locating and pendulum,as well as the isocenter multi-angle non-coplanar light principle on the chest and abdomen precise radiation therapy,the thoracic-abdominal tumor precise positioning puncture devices was developed with the use of tumor center spatial location method and radiotherapy positioning coordination system transfer method of radiation oncology treatment planning system (TPS).Results The device was applied to puncture chest tumor,reaching 100％ accuracy rate of one time biopsy.The average error in the space between tumor center (pre-puncture points) and actual puncture point (the actual arrival at the needle tip) was 2.59 mm,and the average displacement in the space distance of skin marker was 0.56 mm.Conclusion Using the newly developed thoracic-abdominal tumor precise positioning puncture devices can reduce the dependence on doctor's experience and the technique,as well can significantly improve the accuracy rate of puncture.As a result,it is a promising device which can be put in application.