ABSTRACT
OBJECTIVE:To explore the transform of the medicinal properties of Coptis chinensis-Cinnamomum cassia couplet medicines before and after mixing. METHODS:Mice were randomly divided into normal group,weak constitution (induced by food restriction and swimming) group,weak constitution+C. chinensis group,weak constitution+C. cassia group,weak constitu-tion+couplet medicine group,prosperous constitution (induced by high protein diet) group,prosperous constitution+C. chinensis group,prosperous constitution+C. cassia group and prosperous constitution+couplet medicine group,with 10 mice in each group. After modeling,each group was given relevant medicine intragastrically by 20 g(crude drug)/(kg·d),and normal group was giv-en equivalent volume of normal saline intragastrically for consecutive 7 days. The proportion of rats remained in high-temperature ar-eas was recorded. Organ index,biochemical index(Na+/K+-ATPase,Ca2+/Mg2+-ATPase),total antioxidant capacity(T-AOC),SOD activity,the serum content of noradrenalin and dopamine were detected. RESULTS:Compared with normal group,proportion of mice in high-temperature area increased in weak constitution group,while liver index,spleen index,renal index,biochemical in-dex activity or content decreased(P<0.05);the change of above index in prosperous constitution group were opposite to weak con-stitution group;above index had statistical significance except for the proportion of mice in high-temperature area(P<0.05). Com-pared with weak constitution/prosperous constitution group,the proportion of mice remained in high-temperature area and liver in-dex increased in weak constitution/prosperous constitution+C. chinensis group,while biochemical index activity or content de-creased(P<0.05);the change of above index in weak constitution/prosperous constitution+C. cassia group were opposite to C. chi-nensis group,spleen index and renal index increased(P<0.05);the proportion of rats remained in high-temperature area increased in weak constitution/prosperous constitution couplet medicine group,and biochemical index activity or content decreased,among which total antioxidant activity decreased significantly(P<0.05). CONCLUSIONS:The couplet medicine manifests the“cold na-ture”after C. chinensis and C. cassia equally paired,because“hot nature”degree of C. cassia is lower than“cold nature”of C. chi-nensis.