The cellular immunotherapy of integrated photothermal anti-oxidation Pd-Se nanoparticles in inhibition of the macrophage inflammatory response in rheumatoid arthritis

Reducing the inflammatory response is a major goal in the therapy of rheumatoid arthritis (RA). Herein, we integrated palladium nanoparticles (Pd NPs) with selenium nanoparticles (Se NPs) and obtained a multiple nanosystem (Pd@Se-HA NPs) that could simultaneously scavenge hydroxyl radicals (⋅OH) and provide a photothermal effect. The Pd@Se-HA NPs were constructed by a simple self-assembly method in which Se NPs were electrostatically bonded to Pd NPs; hyaluronic acid (HA) was linked to the NPs by ester bonding to provide macrophage targeting ability. The experiments show that the combined therapy of eliminating ⋅OH with Se NPs and utilizing PTT with Pd NPs could effectively reduce the inflammatory response in macrophages more effectively than either individual NP treatment. In addition, the outer layer of HA could specifically target the CD44 receptor to enhance the accumulation of Pd@Se NPs at the lesion, further enhancing the therapeutic effect. After treatment for 15 days, the Pd@Se-HA NPs nearly eliminated the inflammatory response in the joints of mice in an induced RA model, and prevented joint damage and degradation.

Tailored core‒shell dual metal-organic frameworks as a versatile nanomotor for effective synergistic antitumor therapy

Malignant tumor has become an urgent threat to global public healthcare. Because of the heterogeneity of tumor, single therapy presents great limitations while synergistic therapy is arousing much attention, which shows desperate need of intelligent carrier for co-delivery. A core‒shell dual metal-organic frameworks (MOFs) system was delicately designed in this study, which not only possessed the unique properties of both materials, but also provided two individual specific functional zones for co-drug delivery. Photosensitizer indocyanine green (ICG) and chemotherapeutic agent doxorubicin (DOX) were stepwisely encapsulated into the nanopores of MIL-88 core and ZIF-8 shell to construct a synergistic photothermal/photodynamic/chemotherapy nanoplatform. Except for efficient drug delivery, the MIL-88 could be functioned as a nanomotor to convert the excessive hydrogen peroxide at tumor microenvironment into adequate oxygen for photodynamic therapy. The DOX release from MIL-88-ICG@ZIF-8-DOX nanoparticles was triggered at tumor acidic microenvironment and further accelerated by near-infrared (NIR) light irradiation. The

Advances in the studies of core-shell-type lipid-polymer hybrid nanoparticles / 药学实践杂志

Core-shell-type lipid-polymer hybrid nanoparticles (CSLPHNs) are composed by a biodegradable polymeric core coated with single or multiple layers of biomimetic lipids ,which combine the benefits of polymeric nanoparticles and lipo-somes .CSLPHNs have the advantages of small particle size ,high drug loading ,good biocompatibility and controlled release capability .It has wide applications as a novel drug delivery system .This review gives a brief introduction in characteristics , preparation methods and applications of CSLPHNs ,specifically summarizes the developments in the fields of ophthalmic drug delivery ,tumor therapy and medical diagnostic imaging .

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry / 分析化学

Metallothionein ( MT) is a low-molecular-weight protein with high inducibility and binding ability with metal ions. Therefore, MT is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. But the traditional process of its enrichment and identification is time-consuming and complicated. Herein, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles ( Fe3O4@Au NPs) . The nanoparticle possessed the advantages such as fast response to magnetic fields and optical properties attributing to Fe3O4and Au nanoparticles separately. Fe3O4@Au nanoparticles were used to enrich MT simply through Au-S interaction, and the purified proteins were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF/MS) . The results in this work showed that the Fe3O4@Au nanoparticles could directly enrich MT from complex solutions and the detection limit could be down to 10 fg/mL.

Preparation and characterization of Indigo Naturalis-Alumen composite particles based on particle design principle / 中草药

Objective: To prepare and characterize Indigo Naturalis-Alumen (INA) composite particles with core-shell structure based on the particle design principle. Methods: The preparation time of shell particles and composition time of composite particles were studied using particle size and contact angle as evaluation indexes, respectively. Four kinds of powders including Indigo Naturalis fine powder, Alumen fine powder, INA mixture, and INA composite particles were prepared respectively; Their properties such as particle size, powder color, microstructure, distribution of surface elements, and crystal structure were compared, so as to study the core-shell structure of composite particles. Results: During the period of the preparation, the shell particles were added into vibromill and ground for 17 min separately, and then the core particles were added and ground with shell particles together for 5 min. There was no difference between the composite particles and shell particles in surface wettability. The median diameter (d0.9) of the composite particles was 32.818 μm, which was much smaller than that of the mixture. The color and surface elements distribution were highly similar to the shell particles. In SEM, the microstructure that the surface of big particles coated with a large number of small particles could be observed. In X-ray diffractogram, many characteristic absorption b ands of the core particles were disappeared or decreased. Conclusion: INA composite particles with core-shell structure are prepared successfully, the surface properties are similar to the shell particles and are different with the core particles.