Study on Metabolomics of Stable Angina Pectoris of Coronary Artery Heart Disease with Spleen Deficiency and Phlegm Turbidity Syndrome Based on LC-MS / 世界科学技术-中医药现代化

Objective To analyse the differential metabolites and related metabolic pathways in stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome by serum metabolomics.Methods This study observed 60 patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome and 60 healthy volunteers in the same period.Liquid chromatography-mass spectrometry(LC-MS)was performed on the serum metabonomics.The differential metabolites were identified by multivariate statistical analysis of the original spectrogram and original data,and enrichment analysis of KEGG metabolic pathway was analyzed.Results A total of 60 patients in the group of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome participated in the study,and a total of 60 healthy volunteers in the control group participated in the study.There was no statistical difference in general information and biochemical indicators between the two groups(P>0.05);Eighteen differential metabolites were found respectively,including phenylacetaldehyde,orthophosphate,guanosine,diethyl phosphate,2-dehydro-d-gluconate,guanine and 5-(2-hydroxyethyl)-4-methylthiazole down-regulated expression,taurocholate,2-propylglutaric acid,8-amino-7-oxononanoate,l-tyrosine,s-sulfo-l-cysteine,cyclohexanecarboxylic acid,porphobilinogen,(r)-acetoin,octanoylglucuronide,melatonin and solanine up-regulated expression,involving phenylalanine metabolism,thiamine metabolism,purine metabolism.Conclusion The differential metabolites reveal the metabolic essence of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome from the micro level,and can provide clues for clinical early warning of patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndromet.

Research progress on related risk factors of premature coronary artery heart disease / 上海交通大学学报（医学版）

Coronary artery heart disease (CHD) is a common clinical disease with high morbidity and mortality. In recent years, with the improvement of people's living standards and the changes of lifestyle, the age of CHD tends to be younger. Based on the present research situation at home and abroad, this paper explores the hereditary factors, clinical risk factors, inflammatory indexes and new inflammatory markers related to the diagnosis and treatment of premature CHD (PCHD). And the particularity of PCHD in women is analyzed. It is pointed out that PHD is dominated by hereditary factors, with multiple risk factors participating together including psychological factors, and closely related to inflammatory indicators. The emergence of new inflammatory markers provides certain value for the prediction, diagnosis and treatment of PCHD.

The correlation between thyroid function and the severity of coronary artery lesions / 中国介入心脏病学杂志

Obiective Tend to explore the correlation between thyroid function and the severity of coronary artery lesions in chinese coronary heart disease(CHD)patients. Methods A total of 558 patients who underwent coronary angiography at the cardiology department of Peking University First Hospital from January 2013 to June 2015 were enrolled in this retrospective study. All patients were divided into coronary heart disease group and non-coronary heart disease group. Thyroid hormone levels were tested in all patients before angiography,and clinical characteristics,lipid profiles and SYNTAX scores were also obtained. Results Of the 558 patients,409 were diagnosed of CHD(73.3％),and among them,5 patients were hyperthyroid/subclinical hyperthyroid(1.2％),13 patients were hypothyroid/subclinical hypothyroid(3.2％),14 patients had euthyroid sicknesssyndrome(ESS)(3.2％),377 patients were euthyroid(92.2％). Among the 149 non-CHD patients(26.7％),3 patients were subclinical hyperthyroid(2.0％),8 patients were hypothyroid/subclinical hypothyroid(5.4％),2 patients were ESS(1.3％),172 patients were euthyroid(91.3％). The proportion of patients with ESS in the CHD group was significantly higher than that of the non-CHD group (3.4％ vs. 1.3％,P=0.018). Except for the patients with ESS,FT3 level was significantly lower the in CHD group than that in the non-CHD group[(4.52±0.57)pmol/L vs.(4.65±0.63)pmol/L,P=0.015]. There were no significant differences in FT4,T3,T4 levels between the two groups(P>0.05). In the CHD group,there was an association between the SYNTAX score groups and free triiodothyronine(FT3)levels(F=6.260,P=0.002). A significant correlation was also observed between the FT3 level and the number of coronary artery lesions(F=5.691, P=0.004). There was no correlation between the SYNTAX score groups or number of coronary artery lesions and thyroid hormone levels,respectively. There were no correlations between lipid profiles and thyroid function. Patients were further divided into three subgroups according to their serum TSH levels. The prevalence of CHD is significantly higher in the subgroup with elevated TSH(85.7％)than in the subgroup with normal TSH(68.6％, P=0.022). Conclusions FT3 level is weakly associated with the severity of CHD. Higher TSH level may be a risk factor of CHD.

Research progress of Guanxin Danshen formula and its effective components in treating coronary artery heart disease / 中国中药杂志

Coronary artery heart disease (CHD) is one of the common cardiovascular diseases in clinical. The morbidity and mortality of CHD recently continue increasing in our country, which has aroused wide attention. Many studies confirm that traditional Chinese medicine has better therapeutic effect on CHD. Guanxin Danshen formula, widely used in the treatment of CHD, consists of Salviae Miltiorrhizae Radix et Rhizoma, Notoginseng Radix et Rhizoma and volatile oil from Dalbergiae Odoriferae Lignum, and has the efficacy in promoting blood circulation to resolve stasis, regulating the circulation of Qi and alleviating pain. This review summarized the pharmacologic effects and mechanism of Guanxin Danshen formula and its effective components in the treatment of CHD to provide reference for its fundamental research and clinical application.

Fatores de risco cardiovasculares em trabalhadores de enfermagem em um centro de referência no sul do brasil / Factores de riesgo cardiovascular en trabajadores de enfermería en un centro de referencia en el sur de brasil / Factors of heart attack risks in workers of nursing in a center of reference in southern Brazil

Introdução: Atualmente as doenças cardiovasculares, em especial, a doença arterial coronariana (DAC) representa 35 por cento das causas de morte das doenças vasculares. Objetivos: Identificar os fatores de risco (FR) modificáveis para doença arterial coronariana em trabalhadores de enfermagem que atuam em Pronto Socorro, Centro Obstétrico, Centro Cirúrgico e Unidades de Terapia Intensiva (Pediátrica, Adulta, Neonatal); correlacionar os resultados entre as categorias de enfermagem, turnos e setores de trabalho. Métodos: Estudo transversal contemporâneo. Foi aplicado um questionário com finalidade de rastrear fatores de risco, pressão arterial (PA), dados antropométricos, glicemia de jejum, colesterol total- Hdl e Ldl, triglicerídeos e aplicação do inventário de sintomas de estresse de adultos de LIPP ( ISSL). Resultados: Foram incluídos 80 participantes do estudo, 86 por cento eram mulheres com idade média de 37,7 +/- 8,2 anos, 26 por cento eram enfermeiros, 56 por cento eram técnicos de enfermagem e 17 por cento eram auxiliares de enfermagem. O setor da UTI prevaleceu em número de trabalhadores (45 por cento) e o diurno foi o turno predominante (41,2 por cento). Os FR mais relevantes foram história familiar (86,3 por cento) em 1º grau de parentesco, o sedentarismo (55 por cento), colesterol total alto (6,3 por cento) e limítrofe (26,3 por cento),estresse (53,8 por cento) e o sobrepeso (56,3 por cento). Conclusão: Nosso estudo mostrou que esta população possui risco aumentado para DCV. O acompanhamento desta amostra poderá nos trazer resultados surpreendentes por tratar-se de uma população jovem com prevalência de resultados limítrofes. Programas deverão ser adotados para auxiliar a promoção à saúde e a mudança do estilo de vida iniciando precocemente, na infância.

Introduction: Nowadays the cardiovascular diseases (CVD), specially the Coronary Heart Disease (CHD), or Coronary Artery Disease (CAD), represent 35 percent of causes of death of vascular diseases. Objectives: To identify the risk factors that can be modifed for CHD/CAD in Nursing Workers who act at the Emergency Room, Obste-tric Center, Center and Surgical Intensive Care Units (Pediatric, Adult, Neonatal); to correlate these results with the different categories of nursing as well as with their work shifts and sectors. Methods: Cross-study analysis. A questionnaire was applied aiming at tracking risk factors, blood pressure, anthropometric data, fasting glyce-mia, total cholesterol (HDL and LDL), triglycerides, as well as Lipp´s Inventory of Stress Symptoms in Adults. Results: Out of 80 participants of the study, 86 percent were women aged about 37,7 +/- 8,2 years; 26 percent were Nurses, 56 percent were Nursing Technicians and 17 percent were Nursing Assistants. Most Nursing workers belong to the sector of the Intensive Care Unit (45 percent) and the daytime was the predominant shift (41,2 percent). The most relevant risk factors were the family history (at 1st degree) (86,3 percent), the sedentarism (55 percent), total high cholesterol (6,3 percent) and borderline cholesterol level (26,3 percent, stress (53,8 percent) and overweight (56,3 percent). Conclusion: Our study has shown that population presents an increased CVD risk. Monitoring this sample can reveal surprising results in the future since it refers to a young population with predominance of bordering results. Programs should be adopted to facilitate health promotion towards a healthier life style since early in the childhood.

Introducción: Actualmente las enfermedades cardiovasculares (ECV), en especial la enfermedad arterial coronaria (EAC), representan 35 por ciento de las causas de muerte de las enfermedades vasculares. Objetivos: Identificar los factores de riesgo (FR) modificables para la enfermedad arterial coronaria en los trabajadores de enfermería que actúan en la Sala de Emergencia, Centro Obstétrico, Centro Quirúrgico y las Unidades de Cuidados Intensivos (pediátrico, adulto, neonatal); correlacionar los resultados entre las categorías del oficio de enfermería, los turnos y los sectores de trabajo. Métodos: Estudio transversal contemporáneo. Se aplicó un cuestionario para identificar los factores de riesgo, la presión arterial, los datos antropométricos, la glicemia en ayunas, el colesterol total (HDL y LDL), los triglicéridos; también se aplicó el inventario de síntomas del estrés de los adultos, de Lipp. Resultados: De los 80 participantes del estudio, 86 por ciento eran mujeres con la edad media de 37.7 +/- 8.2 años; 26 por ciento eran enfermeros; 56 por ciento eran técnicos de enfermería y 17 por ciento eran auxiliares de enfermería. El sector de UTI prevaleció en número de trabajadores (45 por ciento) y el diurno era el turno predominante (41.2 por ciento). Los factores de riesgo más prevalentes eran la historia familiar (86.3 por ciento) en primero grado de relación, el sedentarismo (55 por ciento), el colesterol total alto (6.3 por ciento) y el colesterol limítrofe (26.3 por ciento), el estrés (53.8 por ciento) y el exceso de peso (56.3 por ciento). Conclusión: Nuestro estudio ha mostrado que la población presenta un FR aumentado de CVD. El monitoreo de esta muestra puede revelar resultados sorprendentes en el futuro ya que se refere a una población joven con el predominio de resultados limítrofes. Deben ser adoptados programas para facilitar la promoción de salud hacia un estilo de vida más sano desde temprano en la infancia.

Study on the expression of free Ca2+ and AngiotensinⅡ1 type receptor in vascular smooth muscle cells of hypertensive coronary artery diseases / 中国综合临床

Objective To observe the expression of free Ca2+ and Angiotensin Ⅱ 1 type receptor(AT1 R)in vascular smooth muscle cells(VSMCs)of patients with hypertensive or normotensive coronary artery diseases(CAD).Methods During the coronary artery bypass graft operation,the surplus saphenous vein of patients who admitted to our Cardiac Surgery Department was collected and cultured.All patients were divided into hypertensive or normotensive group.Free Ca2+ in the cultured human VSMCs was determined by confocal laser scanning microscope(CLSM)after different kinds of AngiotensinⅡ being added,respectively.Total RNA was extracted from cultured VSMCs.Then RT-PCR was conducted for the observation of the expression of AT1R in both groups.Results Ca2+in human VSMCs rapidly increased when stimulated by Angtensin Ⅱ in two groups.After stimulated by Angiotensin Ⅱ,both free Ca2+ level and the expression of AT1R in VSMCs of hypertensive patients were higher than those of the normotensive patients(P＜0.05).Conclusion There are certain changes of free calcium in the cultured human vascular smooth muscle cells,when stimulated by Angiotensin Ⅱ.There are also difierences in AT1R expression between hypertensive CAD patients and normotensive CAD patients.