ABSTRACT
Objective: To study the change regulation of nine compounds(oxypaeoniflorin, albiflorin, paeoniflorin, benzoyl-paeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin) in Peony and Licorice decoction, and analyze the correlation between therapeutical effects and active components alignment. Methods: Classical prescription Peony and Licorice decoction (Shanghanlun) were taken as the carrier, five different combinations of Peony and Licorice decoction were used as the control, and the content was determined by HPLC-DAD. Results: The contents of nine compounds of different combinations of Peony and Licorice decoction was(mg/ml): Shanghanlun 1.92, 0.90, 3.51, 0.53, 8.35, 3.16, 0.77, 0.77 and 0.15; Chuanxinshiyongfang 0.92, 0.45, 1.62, 0.37, 0.78, 0.34, 0.06, 0.10 and 0.01, Zhushijiyanfang A 1.59, 0.82, 3.28, 0.49, 3.15, 1.10, 0.27, 0.44 and 0.08, Zhushijiyan-fang B 1.14, 0.52, 2.12, 0.41, 2.92, 0.97, 0.22, 0.36 and 0.06, Weishijiacangfang 1.38, 0.59, 2.70, 0.43, 2.40, 0.35, 0.06, 0.10 and 0.01, Yi-menbafang 1.52, 0.67, 2.91, 0.45, 3.00, 1.07, 0.24, 0.42 and 0.07. Conclusion: 1. The change regulation of the nice compounds was Shang-hanlun>Zhushi-jiyanfang A > Yimenbafang, oxypaeoniflorin, albiflorin, paeoniflorin and benzoylpaeoniflorin in Zhushijiyanfang B Weishijia cangfang. chuanxinshiy-ongfang has the the lowest content of the nine compounds. 2. Total contents and proportion of the nine compounds are different due to different compatibility. The clinical curative effect is also different. Total contents of the nine compounds in Shanghanlun were 20.06 mg/ml which is used to relieve pain in the legs, in Zhushijiyanfang A was 8.72 mg/ml which is used to relieve pain caused by diabetes, in Zhushijiyanfang B was 8.72 mg/ml which is used to treat foot weakness, in Chuanxinshiyong-fang was 4.65 mg/ml which is used to treat wet beriberi, in Weishijiacangfang was 8.02 mg/ml which is used to treat wet beriberi, in Yimenbafang was 10.35 mg/ml which is used to treat peratodynia.