ABSTRACT
OBJECTIVE: To explore the therapeutic effect of compound Lingzhi cream for mild frostbite on rabbit ears. METHODS: Rabbit ears were immersed into alcoholic freezing chamber at -24.5°C to cause mild frostbite, then the therapeutic effect of compound Lingzhi cream of different formula was explored after smearing the cream on the injured site, using appearance change, swelling and distension degree, survival area and pathological change of the ears as the observation index. RESULTS: Compound Lingzhi cream formula 2 was effective on mild frostbite of rabbit ears to some extend, but the effect was not as obvious as those of compound Lingzhi cream formula 3 and 4. CONCLUSION: The effective dosage of compound Lingzhi cream on mild frostbite of rabbit ears is 0.5 g crude Lucid Ganoderma and 0.25 g crude Cowherb Seed in 1 g cream. The optimal dosage is 1 g cream containing 1 g crude Lucid Ganoderma and 0.5 g crude Cowherb Seed. The therapeutic effect would not further increase when increasing the drug dosage. Copyright 2012 by the Chinese Pharmaceutical Association.