Construction of recombinant nonstructural P_2C plasmid in coxsackie B4 virus / 中国免疫学杂志

Objective:To construct the recombinant nonstructural P_2C plasmid in coxsackie B4 virus.Methods:Using the RT-PCR,we fishing the nonstructural protein P_2C cDNA fraction from Coxsackie B4 virus(CVB4) and cloning into the PUCm-T vector, then transfer it into E.coli to construct the recombinant plasmid.Results:Using the total RNA of Coxsackie B4 virus as model, we amplied 987 bp fraction and cloned into PUCm-T vector identifying with BamH Ⅰ and Hind Ⅲ double enzyme.Its products were consistent with nonstructural protein P_2C PCR productions.Conclusion:The recombinant nonstructural P_2C plasmid was constructed succesfully in coxsackie B4 virus.