ABSTRACT
The crude extracts from stem bark of Crataeva nurvala Buch.-Ham containing naturally occurring antioxidants were screened for their most abundant phytosecondary metabolite, antiproteolytic and cytotoxic properties. The antioxidant activity of the extracts was determined by DPPH radical scavenging activity, Ferric reducing power and β Carotene linoleic acid assay. The antiproteolytic activity of crude extracts was determined by inhibition of trypsin-induced hydrolysis of BSA. The IC50 of cytotoxic effect on HepG2 and B16F0 cell line was investigated using MTT assay. Preliminary phytochemical analysis exhibited the presence of terpenoids as a major phytochemical reflecting higher antioxidant activity in a dose dependent manner. The PEECN extract showed good antiproteolytic activity with 26.04% inhibition on BSA. The IC50 values of PEECN against HepG2 and B16F0 cells using MTT assay were determined to be 34.67 ± 1.10 μg/ml and 49.43 ± 4.778 μg/ml after 48 h, respectively. These results demonstrate that the petroleum ether extract containing most terpenoid fraction exhibited potential antiproteolytic and cytotoxic activity against hepatocellular carcinoma and mouse melanoma in vitro through inhibition of proliferating cancerous cells.
ABSTRACT
The aim of the present study is to carry out the quantitative estimation of lupeol in poly herbal formulations by HPTLC and to study the invitro antiinflammatory activity study of Crataeva nurvala bark. The bark of Crataeva nurvala was selected, dried, powdered and extracted with petroleum ether, diethyl amine and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of lupeol was found to be 0.49 ± 0.03.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 50 and 300ng/spot respectively for HPTLC. The anti-inflammatory activity was highest in petroleum ether extract when compared to other extracts when evaluated by using ibuprofen as the control. This shows that the polyherbal formulation and medicinal plants containing lupeol as the active constituent has medicinal value and that they may be utillised for their anti-inflammatory properties.
ABSTRACT
Crataeva nurvala Buch-Hum (Varuna) is well known traditional Indian medicinal plant used to treat various ailments in particular urolithiasis. During last two decades, numerous ethno-pharmacological and scientific reports have been cited in the literature to support its multi-directional therapeutic potential. The plant is rich in alkaloids, saponins, triterpenes, tannins, flavanoid glycosides, glucosinolates and phytosterols. The review emphasizes primarily on folkloric uses, biological activities of isolated compounds, pharmacological activities of the extracts, clinical studies and safety profile of Crataeva nurvala to provide a comprehensive data for researchers to hit upon new chemical entity responsible for its claimed traditional uses.
ABSTRACT
Triterpenoids and phytosteroids from stem bark of Crataeva nurvala buch ham.