Control químico y biológico de larvas de Aedes aegypti en la costa norte de Jalisco, México / Chemical and biological control on Aedes aegypti larvae in the northern coastal region of Jalisco, Mexico

Introducción: el aumento de enfermedades transmitidas por mosquitos se ha asociado a cambios globales: el crecimiento de la población, la urbanización y el cambio climático. Dentro de las alternativas para evitar epidemias están el control químico y biológico. Objetivos: determinar las concentraciones efectivas de tres compuestos químicos para el control de larvas de Aedes aegypti en la región costa norte de Jalisco, México, y evaluar la capacidad predatoria de diversas especies acuáticas sobre larvas de mosquitos. Métodos: se evaluaron cinco concentraciones de temefos (1,5; 1; 0,1; 0,05 y 0,01 g/L), hipoclorito de sodio (5,5; 0,55; 0,05; 0,005 y 0,0005 g/L) y detergente (10; 5; 1; 0,5; 0,1 g/L). Se determinó la muerte de las larvas a 1, 3, 6 y 24 h y se calculó la CL50. Se evaluaron cuatro especies de peces, un crustáceo y una larva de díptero culícido. Se les suministraron diferentes cantidades de larvas (5, 10, 30, 50 y 80) por quintuplicado y se registró su consumo a diferentes tiempos (1, 3, 5, 9 y 24 h). Resultados: el temefos provocó el 100 por ciento de mortalidad en todas las concentraciones probadas. El NaClO provocó mortalidad del 100 por ciento en las dos concentraciones más altas. El detergente fue más eficiente a las tres concentraciones mayores. Los peces demostraron un consumo de casi el 100 por ciento en la mayoría de las densidades probadas. La larva de díptero culícido demostró un consumo cercano al 80 por ciento, el crustáceo solo consumió el 53 por ciento Conclusiones: el uso del temefos debe continuar siendo el larvicida químico de elección en Puerto Vallarta, México. La utilización de peces nativos se sugiere como adecuada para el control biológico(AU)

Introduction: the increase of diseases transmitted by mosquitoes has been associated to global changes such as the population growth, urbanization and climate change. Among the alternatives to avoid epidemics are the chemical and biological control. Objectives: to determine effective concentrations of three chemical compounds to control Aedes aegypti larvae in the northern coastal region of Jalisco and to evaluate the predatory ability of various aquatic species on mosquito larvae. Methods: five concentrations of temephos of (1.5, 1, 0.1; 0.05 and 0.01 g/L), sodium hypochlorite (5.5, 0.55, 0.05, 0,005 and 0.0005 g/L) and detergent (10, 5, 1, 0.5, 0.1 g/L) were evaluated. Larval death was estimated at different times (1, 3, 5, 9 and 24 h) and the LC50 was calculated. Four fish species, one crustacean and one dipteral culicid larva were evaluated. All were provided with different numbers of larvae (5, 10, 30, 50 and 80) five times and the larval consumption was recorded at different times (1, 3, 5, 9 and 24 h). Results: temephos caused 100 percent mortality of larvae in all tested concentrations. The NaClO caused 100 percent mortality at the two highest concentrations. The detergent was more efficient at the three higher concentrations. Fish proved to be efficient predators of larvae, as they consumed almost 100 percent of larvae in most of tested densities. The diptera culicid larva reached around 80 percent consumption whereas crustacean consumed only 53 percent. Conclusions: the use of temephos should remain the chemical larvicide of choice in Puerto Vallarta region. Native fish are suggested to be used as appropriate biological control agents(AU)

Vigilancia entomoepidemiológica de la especie Anopheles albimanus en el municipio de Santiago de Cuba / Entomological and epidemiological surveillance of the Anopheles albimanus species in Santiago de Cuba municipality

Se efectuó un estudio observacional, descriptivo y transversal, a fin de caracterizar algunas variables entomológicas de la vigilancia de culícidos transmisores de paludismo en Santiago de Cuba, desde 2006 hasta 2010. El universo de estudio se agrupó según los métodos establecidos para las colectas larvales de Anopheles albimanus. Se realizaron 602 encuestas larvarias donde existían focos de culícidos sujetos a vigilancia. Finalmente se demostró que 19,6 % de los criaderos fueron positivos a la especie antes citada. Asimismo, la densidad larvaria general fue de 13,4 larvas/m² y la específica de 1,8 larvas/m², con una tendencia al incremento de la positividad en estos criaderos. Se recomendó mantener una estrecha vigilancia en las áreas donde circulaba este vector.

An observational, descriptive and cross-sectional study was carried out, in order to characterize some entomological variables of the surveillance of culicids transmitting malaria in Santiago de Cuba, from 2006 to 2010. The study universe was formed according to the established methods for the larval collections of Anopheles albimanus. There were 602 larval interviews carried out in the culicids breeding under surveillance. Finally it was demonstrated that 19.6 % of the breedings were positive for the above mentioned species. Also, the larval general density was 13.4 larvae/m² and the specific of 1.8 larvae/m², with a tendency to the increase of positive results in these breedings. It was recommended to maintain a closed surveillance in the areas where this vector circulated.

Wing diagnostic characters for Culex quinquefasciatus and Culex nigripalpus (Diptera, Culicidae)

Wing diagnostic characters for Culex quinquefasciatus and Culex nigripalpus (Diptera, Culicidae). Culex quinquefasciatus and Culex nigripalpus are mosquitoes of public health interest, which can occur sympatrically in urban and semi-urban localities. Morphological identification of these species may be difficult when specimens are not perfectly preserved. In order to suggest an alternative taxonomical diagnosis, wings of these species were comparatively characterized using geometric morphometrics. Both species could be distinguished by wing shape with accuracy rates ranging from 85-100 percent. Present results indicate that one can identify these species relying only on wing characters when traditional taxonomical characters are not visible.

Caracteres alares diagnósticos de Culex quinquefasciatus and Culex nigripalpus (Diptera, Culicidae). Culex quinquefasciatus e Culex nigripalpus são mosquitos de interesse à saúde pública e que podem ocorrer em simpatria em ambientes urbanos e semi-urbanos. A identificação morfológica dessas espécies pode ser difícil nos casos em que os espécimes não estão bem preservados. Com o intuito de sugerir um método diagnóstico taxonômico, asas de indivíduos dessas espécies foram caracterizadas comparativamente utilizando-se morfometria geométrica. Ambas as espécies puderam ser distinguidas pela forma alar com índices de confiabilidade entre 85-100 por cento. Os resultados presentes indicam que é possível identificar essas espécies baseando-se apenas no formato alar, nos casos em que os caracteres taxonômicos tradicionais não são visíveis.