ABSTRACT
@#There are a variety of ways for isolation and selective cultivation of retinal microvascular endothelial cells <i>in vitro</i>, in which the newly reported tissue culture with collagenase digestion method are widely used for simple, economical, high purity and good activity of obtained endothelial cells. As a result of the limitation of person eye donor, researchers often use rat retinal microvascular endothelial cells that are highly homologous to human beings to experiment <i>in vitro</i>. In this paper, the methods of culturing retinal microvascular endothelial cells <i>in vitro</i> will be elaborated from the aspects of animal species, added factors, cell purification and identification <i>in vitro</i>. At the same time, the advantages and disadvantages of these methods in the process of operation are briefly described, which can provide a reference for the researchers to select suitable cultivation methods.
ABSTRACT
AIM:To cultivate the exoerythrocytic stage of Plasmodium yoelii in vitro and to study some involved affecting factors.METHOD:In vitro cultivation.RESULTS:The monolayer hepatocytes grown in 1 6 - mm plastic cell culture dishes were inoculated with sporozoite sus- pension prepared from Anopheles stephensi mosquitoes for48hours.At a final density of2? 1 0 4 cells per well,the infection rate of hepatocyte,cultured in medium supplemented wit15 % bovine serum,was 0 .0 35? 0 .0 1 3% ,the diameter of the nearly mature EEF of Plas- modium yoelii was up to40 .3? 31 .6 ?m,and contained more than1 0 0 nuclei,the number of EEF might be4- 1 0 /cm2 .An intraperitoneal inoculation of the EE schizonts to mice could induce parasitemia.At a final density of0 .5? 1 0 4 or4? 1 0 4 cells per well or the hepatocytes cultured in medium supplemented with1 0 % bovine serum,no EEF could be observed.CON- CL USION:The density of hepatocytes and culture medium are important for the cultivation of the EE stage of Plasmodium yoelii.This procedure will lay foundation for the further studies of the sporozoite invasion,the development of EEF and the affecting factors in- volved.
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ObjectiveTo explore the poss ib le mechanisms of killing effects of serum from Microtus fortis to Schistosoma japonicum schisto somula in vitro. MethodsSerum was separated into protein fraction and
ABSTRACT
The article reports the result of the observation on the ultrastructure of the tegument and digestive tract of Schistosoma japonicum schistosomula cultured 130 days in vitro in 841 medium,and the 42-day-old adults from infected animal cultured 23 days in 851 medium by transmission electron microscopy.The results showed that the ultrastructure of the tegument including sensory papillae and flame cells was normal.The matrix seemed to have no vacuolization.The surface of the esophagus was highly rugous.Many laminae and lipid droplets filled the lumen of the digestive tract and the tubules-like depressions from basal lamina were occured.All these structures seemed to have less variation as compared with the prior authors' data from normal worms.It is considered that the above two media which we cesigned were suitable for the growth and development of the schistosomes due to the tegument and digestive tract maintained their metabolic function in the long-term cultivation in vitro.(Figs 1-10).