ABSTRACT
This study aims to develop the pre-column derivatization high performance liquid chromatography(HPLC) method for the determination of 16 kinds of amino acids in Eucommia ulmoides leaves, and compare the content of amino acids in the leaves harvested at different time and under leaf-oriented cultivation mode(LCM) and arbor forest mode(AFM). The HPLC conditions are as below: phenyl isothiocyanate(PITC) as pre-column derivatization agent, Agilent ZORBAX C_(18 )column(4.6 mm×250 mm, 5 μm), mobile phase A of acetonitrile-water(80∶20), mobile phase B of 0.1 mol·L~(-1) sodium acetate solution-acetonitrile(94∶6), gradient elution, flow rate of 1.0 mL·min~(-1), injection volume of 5 μL, column temperature of 40 ℃, and detection wavelength of 254 nm. The HPLC profile indicated well separation of 16 kinds of amino acids and the amino acid content in E. ulmoides leaves was up to 16.26%. In addition, the amino acid content in leaves of E. ulmoides under LCM was higher than under AFM. The amino acid content varied with the harvesting time. Through orthogonal partial least squares discriminant analysis, the amino acids of E. ulmoides under LCM and AFM were compared, which can distinguish the leaves under LCM from those under AFM. Principal component analysis was applied to comprehensively score the amino acids of E. ulmoides leaves. The results showed that the score of leaves under LCM was higher than that under AFM. Nutritional evaluation results indicated that the proteins in E. ulmoides leaves belonged to high-quality vegetable proteins. The established method for the determination of amino acid content is reliable. With the amino acid content as index, the leaf quality of E. ulmoides under LCM is better than that under AFM. This study lays a theoretical basis for the promotion of LCM for E. ulmoides and the development of medicinal and edible products from E. ulmoides leaves.
Subject(s)
Amino Acids/metabolism , Eucommiaceae/chemistry , Chromatography, High Pressure Liquid/methods , Plant Leaves/chemistryABSTRACT
Since the enrollment in 2002, the cultivation model of "4+4" program of clinical medicine in Shanghai Jiao Tong University School of Medicine has been continuously explored and practiced. With the goal of cultivating high-level compound outstanding medical innovative talents with multi-disciplinary cross-capacity, through strengthening the heuristic teaching, establishing the medical-engineering cross-course, emphasizing the training of scientific research ability, and taking teaching reform in the basic clinical single-circulation organ system integration course, we have established a talent training system with the characteristics of Shanghai Jiao Tong University School of Medicine, which is characterized by "thick foundation, strong practice, re-transformation, shaping norms, and international integration", and intend to make further exploration in the field of post-graduation education convergence.
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Based on the requirements of first-class undergraduate talents training in pharmacy in the new era, this paper summarizes and analyzes the law, current situation and actual situation of pharmaceutical talents training in TCM colleges and universities in China, and discusses the reform and practice of first-class pharmaceutical talents training in terms of talent training mode reform, teaching staff construction, curriculum system optimization, teaching mode innovation, quality assurance system construction and innovation and entrepreneurship education in combination with the construction experience of national first-class undergraduate specialty construction point in the pharmacy of Guangzhou University of Chinese Medicine, so as to further improve the quality of personnel training and provide a reference for excellent pharmaceutical personnel training.
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The traditional cultivation ways of socialist core values among medical students existed many short-comings in the aspects of attraction, innovation, influence and so on. The medical students' basic attitude to social-ist core value was positive, but there were some characteristics of lack of initiative, lack of practice force, lack of awareness and so on. The construction of new cultivation ways need the combination of the traditional education and the emerging Internet media, especially mobile Internet terminals and thought, construct the education network of the combination of the school, family and society, and make medical students' socialist core values internalized in the mind and externalized in the practice truly.
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Objective To explore the effectiveness of the new clinical teaching method based on problem leading-in and the reform of clinical talents training mode. Methods 180 clinical interns in de-partment of urology were selected as the subjects, which were randomly divided into experimental group and control group (90 clinical interns in each group). The clinical teaching effects of the two groups were compared by adopting the new clinical teaching methods and the traditional ones. Evaluation indexes con-sists of theoretical scores, operational skills and personal comprehensive quality scores. Results The results of the above three tests in the experimental group were all superior to those of the control group (P<0.05). The satisfaction of the experimental group in teaching method, teaching effect and teacher evaluation was obviously better than that in the control group (P<0.05). Conclusion The implementation of the new clinical teaching method based on problem leading-in is not only beneficial to improve the knowledge skills, com-prehensive quality and teaching satisfaction of clinical medical students, but also to the training of clinicians with high quality. The new clinical teaching method is of great significance for reforming the mode of clini-cal medical talents.
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Medical students' medical ethics education is an important mission of medical college moral education.The current medical ethics cultivation is facing the profound reform of humanistic medical education,the gradual exploration and improvement of medical ethics education system,the development and application of online information platform,the establishment and development of china medical self-media alliance and other opportunities.Meanwhile,it is facing the differences in the motivations of students to study medicine,the diversified values,the sharp decline of professional teams,the deterioration of occupational environment and other challenges.In order to actively deal with opportunities and challenges,medical colleges and universities should establish the longitudinal three-dimensional medical ethics cultivation mode,form the horizontal collaborative pattern of medical ethics education,perfect the dynamic and long-term medical ethics cultivation safeguard mechanism,and improve the evaluation system that combined qualitative and quantitative.
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In the wake of on-the-spot investigation into Chinese major production bases in Fujian, Zhejiang, Jiangxi, Taiwan, Guangxi and Yunnan provinces, and based on relevant literature, the paper systematically elaborates the current researches of botanical origin, major cultivation type, seedling propagation technique as well as cultivation mode of Jinxianlian. The way of seedling breeding mainly includes aseptic seed culture, in vitro propagation, artificial seed and bioreactor propagation, etc. And the planting model mainly includes protected cultivation modes, bionic wild cultivation modes and pot cultivation modes, etc. Further discussions have also been conducted to tackle significant problems existing in the production process of Jinxianlian, based on personal studies of the authors. It has made considerable contributions for the betterment of Jinxianlian's development and improvement.
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Preeminent Doctors Education and Training Plan has put forward an urgent request to reform the five-year clinical undergraduate medical talent training mode and strengthen the professional spirit cultivation for medical students. As a pilot project, our college continuously explored the professional spirit cultivation mode for medical students in preeminent physician class and has almost established the three-dimensional mode of whole process, joint participation, multi approach and stressing practice.
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Guided by the demands of society,we propose a new professional training objectives of medical laboratory technology,which is to cultivate comprehensive talents covering the entire industrial chain of medical laboratory technology with profound foundation,broad caliber,high quality and outstanding ability.According to the training objective,we build up 4321 talents training system and try to carry out preliminary practice and exploration on talent cultivation model from the following aspects,such as the construction of curriculum system,the reform of the teaching contents and methods,training of students' professional skills and entrepreneurial innovation spirit.
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Objective To explore the application of reading education in cultivating nursing students' humanities in vocational college.Methods Nursing students were selected at random and divided into two groups,35 students in the experimental group and 50 in the control group.The students in the experimental group were given the 2-year related instruction through reading activities in the light of the principle enhance general humanities foundation in the first grade and strengthen humanistic features in the second grade.Nursing skill assessment and questionnaire oriented to humanities were used to test the educational effect.Results The result of nursing skill assessment in the experimental group was significantly better than that of the control group.The score of humanities' moral,law,culture and aesthetic dimension and the total score of the experimental group were significantly better than those of the control group.Conclusions Well targeted reading is effective in the cultivation of nursing students' humanities.Meanwhile reading education can serve as an important and beneficial way to improve the cultivation mode of vocational college's nursing humanities.
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With medical system reform advancing,social requirement on comprehensive capability for health service management professionals enhances unceasingly.However,health service management in our country is a new branch of edge-crossed and needs to be improvd and perfected constantly to reduce the shortage in mode construction for talent cultivation. It is meaningful to explore and construct innovative cultivation mode for health management professionals from three aspects,management capability,learning capability and information feedback based on system self-organization principle.
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Modern medical education is characterized by innovative education,but the specialty of medical education determines the complexity of its innovative education.The paper elaborates the construction of cultivation mode of talents of creative pattern in medical universities by infiltrating and implementing the ideas and practice of innovative education into the full process of higher medical education proceeding with such innovative thoughts and quality training links as education concept,teaching mode,knowledge structure,training of methods and ability.
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The author has explored the cultivation mode of medical postgraduates which fits our country need and scientifically adopted the different cultivation mode for postgratuates of different degree and level to find out the cultivation mode that suits medical postgraduate of our university and lay a good basis for cultivating excellent doctors.
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Traditionally the cultivation mechanisms of pathological postgraduates can not fully adapt to the demand of research and society in China,to some extent,which impedes the development of pathology.Combining our practical conditions with overseas experiences,we try to make the beneficial thinking and adjustment on the existing cultivation mechanisms.