ABSTRACT
OBJECTIVE: Our previous study demonstrated that moxibustion could promote skin wound healing in rats with full-thickness cutaneous wounds. The present study was designed to observe the effect of moxibustion on the levels of pro-inflammatory and anti-inflammatory cytokines, and proliferation of vascular endothelial cells, so as to explore its mechanisms underlying facilitating wound-healing. METHODS: A total of 84 adult SD male rats were randomly assigned to normal (n＝6), model(n＝39)and moxibustion(n＝39)groups. The skin wound model was established by removal of a piece of full-thickness skin from the median line of the rats' back (about 2 cm below the shoulder blade). Moxibustion was applied to the surrounding area of the focus for 25 min, once daily for 6 days. The blood samples were collected at day 1, 2, 3 and 5 after modeling for assaying serum contents of IL-1α， IL-1β， IL-6, IL-4, IL-10, IL-13, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophagecolony stimulating factor (GM-CSF, an inflammatory mediator), macrophage inhibitory protein-1α (MIP-1α) and vascular endothelial growth factor (VEGF) with liquid chip methods, and the topical wound tissues were collected after trans-cardiac perfusion with 4% paraforma-ldehyde solution for detecting the expression of CD31 proteins by using immunofluorescence staining. RESULTS: After modeling and in comparison with the model group, the contents of serum IL-1α and IL-6 at the 1st day were significantly increased (P<0.05), but notably decreased at the 3rd day after modeling in the moxibustion group (P<0.05). The contents of serum IL-1β on day 1 and 2 were significantly higher in the moxibustion group than those of the model group (P<0.05). After moxibustion, the contents of serum IL-4 and IL-10 were significantly up-regulated on day 1 after modeling (P<0.05), and obviously down-regulated from the 3rd day on (P<0.05). The contents of serum MIP-1α on the 2nd day and serum VEGF on day 1 and 2, and wound-skin VEGF on day 5 were obviously up-regulated in the moxibustion group in comparison with the model group (P<0.05, P<0.01)．. CONCLUSION: Moxibustion promotes the inflammatory response by regulating the levels of both pro-inflammatory and anti-inflammatory cytokines in the early phase of skin wounds in rats, which may be in favor of the transformation from the inflammatory phase to the proliferation phase in advance, promoting wound healing at last.
ABSTRACT
Objetivo: O presente estudo avaliou o efeito do laser arseneto de gálio-alumínio (GaAsAl) 830nm(30j/cm²) e da pomada Dersani® no processo cicatricial cutâneo de ratos wistar, em relação à proliferação fibroblástica e revascularização. Material e métodos: Foram utilizados 18 ratos wistar adultos jovens, machos, com peso médio de 324 g, provenientes do Biotério do Centro de Ciências Biológicas da Universidade Federal de Viçosa. Foram feitas cinco feridas de 12 mm no dorso dos animais utilizando bisturi. Os animais foram divididos aleatoriamente em três grupos, cada grupo com seis animais: Grupo 1: Controle - os animais tiveram a ferida tratada com salina, Grupo 2:Feridas tratados com laser GaAsAl (830nm) 30J/cm² e Grupo 3: Feridas tratadas com Dersani®. As aplicações foram feitas diariamente durante 20 dias de experimento. O material para análise histológica foi corado com hematoxilina-eosina (HE), fotografados e analisados por meio do programa Image Pro-plus®, por contagem de pontos sob células de interesse. Resultados: Foi observado maior número de fibroblastos nos grupos tratados com o laser GaAsAl e com a pomada Dersani®, quando comparados ao controle no quarto dia do experimento. No entanto, no oitavo dia o grupo tratado com laser apresentou um número significativamente menor de fibroblastos, quando comparado ao controle e ao Dersani®. Em relação à revascularização foi observada diferença significativa entre o laser e o Dersani® no oitavo dia de experimento, em que o Dersani® se mostrou mais efetivo na formação de vasos sanguíneos. Conclusão: O grupo tratado com o laser GaAsAl no quarto dia aumentou significativamente a quantidade de fibroblastos quando comparado ao controle.
Objective: The present work evaluates the effect of the gallium-aluminum arsenide (GaAsAl) (30j/cm²) laser and ointment DersaniTM, on the cutaneous cicatricial process the wistar rats, in respect of fibroblast proliferation and revascularization. Materials and methods: The study made use of 18 wistar rats, young adults, males, with medium weight of 324 g, from the animal house of Centro de Ciências Biológicas e da Saúde (Health and Biological Sciences Center) of Universidade Federal de Viçosa, MG, Brazil. Five 12 mm wounds were made in the dorsal region of the rats using scalpel blades. Animals were separated in 3 groups, each one with six animals. Group 1: Control - Animals had the wound treated with saline; Group 2: Wound treated with GaAsAl (30J/cm²) laser; and Group 3: wound treated with DersaniTM. The applications were made daily during 20 days of experiment. The material for histological analyses was stained with hematoxilin-eosin (HE), photographed and analyzed using the program Image Pro-plusTM through enumeration of points under the cells of interest. Results: It was observed an increase in the number of fibroblasts in the groups treated with GaAsAl 30J/cm² and with DersaniTM ointment when compared to controls in the fourth day of experiment. However, in the eighth day the group treated with laser presented a significant reduced number of fibroblasts when compared to control and DersaniTM groups. In relation to revascularization, significant differences between laser and DersaniTM were observed in the eighth day of the experiment, where to DersaniTM showed to be more effective in the formation of blood vessels. Conclusion: The group GaAsAl laser on the fourth day, there was a significantly greater quantity of fibroblasts compared to control group.