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OBJECTIVE: To identify morphological characteristics of Cyathula officinalis Kuan. and Cyathula capitata(Wall.) Moq.. METHODS: Based on tissue morphology features, C. officinalis Kuan. and Cyathula capitata (Wall.) Moq. were differentiated by using tissue sectioning, optical microscope, scanning electron microscopy and transmission electron microscopy technology. RESULTS: C. officinalis exhibited features, such as main roots, stems composed of four vascular bundles, relative rich trichomes, less green color, however, for C. capitata, hairy roots, stems composed of two vascular bundles and less trichomes were observed. Compared with low altitude condition, high-altitude condition may induce darker green color and formation of yellow spots and osmiophilic granules of leaves for C. capitata. CONCLUSION: It provides a scientific approach to differentiate these two closely related medicinal plants.
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Objective: To reveal the variation regularity of chemical components in crude Cyathula officinalis and its processed products (stir-fried with yellow rice wine and salt), and promote the quality control of the herb. Methods: Agilent Zorbax Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile and 0.1% phosphoric acid solution in gradient elution mode. The detective wavelength was 266 nm and the flow rate was 0.5 mL/min. The fingerprints for 24 herbal samples were set up and 25 peaks were recorded with different retention time and peak areas. Three peaks were successfully identified as puerarin, cyasterone, and daidzein by comparing with the retention time of reference substances. The vectorial angle method was used to evaluate the similarity between fingerprints. The cluster analysis and principal component analysis were applied to studying the HPLC fingerprint and chemical pattern recognition. Results: The two processing techniques, stir-frying with wine and salt, both had significant effect on herbal chemical profiles. The contents of three known active components, puerarin, cyasterone, and daidzein, were not observed except a little increasing of the content of puerarin after the crude herbs were processed with salt. Conclusion: More precise active components of the processed products of C. officinalis need to future study to improve the current quality standard of C. officinalis in Chinese Pharmacopoeia. The method provided by this study is a powerful tool to identify and quality control between crude and processed C. officinalis because of its quantificational and visual evaluation system.
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Objective: To evaluate the suitability of Cyathula officinalis resources grown in Sichuan province, and provide a reference of the monitoring for traditional Chinese medicine resource. Methods: Taking the planting of C. officinalis in Sichuan province as an example, The topography, climate, soil, and vegetation of C. officinalis were researched using remote sensing and GIS techniques, binding environment factors in growing and adopting the analytic hierarchy process (AHP) and spatial analysis method. According to the different classification of index factors, the classification map of the suitable area for C. officinalis was obtained and the field measurements were combined to verify. Results: The C. officinalis resources in Sichuan province were mainly distributed in about 19 cities or autonomous prefectures such as Leshan City, Ya'an City, Yi Autonomous Prefecture of Liangshan, and Yibin City with suitability distribution areas of 3 633.52 km2 approximately and accounting for 0.76% of the total area. According to the field investigation, related documents, and information records, the suitability distribution based on RS and GIS correspond with the actual distribution areas of C. officinalis resources. Conclusion: It is a feasible method to divide the suitability distribution area of C. officinalis Kuan by using RS and GIS as quickly and accurately as possible. It can also provide a support on scientific and date basis to a complete investigation on suitable distribution areas, protection, rational development and utilization of C. officinalis and other medical materials.
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OBJECTIVE: To screen excellent provenance of Radix Cyathulae and ensure the quality of Radix Cyathulae.
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The antifertility and antinidation effects of three extracts (benzol, ethyl acetate and alcohol) of Cyathula Oficinalis Kuan on mice were observed. The results indicate that the benzol extract had significant antifertility action. Its effective rate of antifertility was 100%, when 250 mg/kg was given orally. As compared with control, when 250 mg/kg ethyl acetate extract was given, the rate of antifertility was 63.7%. If 500 mg/kg ethyl acetate extract was given the effective rate of antifertility increased over 90%. However, when 250～500 mg/kg alcohol extract was given, the rate of antifertility was only 40～54%. The study of antinidation showed that the benzol extract (500 mg/kg )could effectively prevent implatation. The rate of antinidation was 100%, and yet the ethyl acetate and alcohol extracts were 66.7% and 40% repectively. The results suggest that the three extracts of Cyathula Officinalis Kuan all had antifertility effect, but the action of benzol extract was more effective than other two extracts.