ABSTRACT
Objective To study the cyfluthrin resistance and potential mechanisms of Anopheles sinensis in Nanchang Chang-bei International Airport,Nanchang City,Jiangxi Province. Methods The resistance levels of the local An. sinensis were de-tected by WHO drug resistance bioassay. During the bioassay,the dying mosquitos were classed as sensitive mosquitos,and the survival ones were classed as resistant mosquitos. The P450 monooxygenase activity and glutathione S-transferase activity were detected and compared between the two groups. At the same time,the death time of each sensitive mosquito was recorded,and the correlations between the death time and the P450 monooxygenase activity and glutathione S-transferase activity were ana-lyzed,respectively. Results The bioassay mortality of the local An. sinensis was 59.5%. The differences of the P450 monooxy-genase activities among the resistant mosquitos,sensitive mosquitos and laboratory sensitive mosquitos had statistical signifi-cances(F=151.89,Psensitive mosquitos>laboratory sensitive mosquitos. The differences of glutathione s-transferase activities among the three groups had no statistical significance(F=0.72,P=0.49). There existed positive correlation between the mosquito death time and the P450 monooxygenase activity,and the regression equation was y=79.479+1.512x with the correlation coefficient of 0.88,while there was no correlation between the mosquito death time and the glutathione S-transferaseactivity. Conclusion The An. sinensis in Nanchang Changbei International Airport has been resistant to cyfluthrin,and the promotion of P450 monooxygenase activity maybe one of the reasons for the resistance.
ABSTRACT
This study was designed to evaluate the nephrotoxic effect of cyfluthrin in rats. Cyfluthrin at 0ppm (control), 100ppm and 200ppm (test-groups) was administered orally for 15 weeks. The micronutrient level (iron, zinc, copper, and selenium), the nutritional status (total carbohydrate, total glucose, total protein, total amino acids, total lipid, and total cholesterol), the lipid peroxidation level (reduced glutathione and thiobarbiturate) and the antioxidant enzyme activities (glutathione peroxidase, glutathione reductase, catalase, and glucose-6-phosphate dehydrogenase) were estimated. The results were statistically compared (p<0.05) with the control. A significant decrease in the organ-to-body ratio was observed in the test groups. The concentration of the micronutrient in the test groups increased significantly. The total carbohydrate, total glucose, total amino acids, total lipids and total cholesterol showed a significant decrease in the test groups, but a significant increase was observed in the tissue protein level of the test groups. Lipid peroxidation was increased in the test groups as indicated by a significant increase in the thiobarbiturate level and a significant decrease in the reduced glutathione level. All the antioxidant enzymes studied increased significantly in the test groups when compared with the control. Cyfluthrin is nephrotoxic under continuous administration in rats.