ABSTRACT
OBJECTIVE:To develop a method for simultaneous determination of catalpol and leonuride in Mongolian medicine Cymbaria dahurica. METHODS:HPLC method was adopted. The determination was performed on XSelect HSS T3 C18 column with mobile phase consisted of acetonitrile-1.0％ phosphoric acid water solution (gradient elution) at the flow rate of 1 mL/min. The column temperature was set at 40℃,and the detection wavelength was set at 203 nm. The sample size was 10μL. Established method was adopted to determine the contents of catalpol and leonuride in 7 batches of C. dahurica samples. RESULTS:The linear range of catalpol and leonuride were 0.17-3.4 and 0.25-5.0 μg (r≥0.9995). The detection limits were 3.57 and 4.72 ng, respectively. The quantitative limits were 12.24 and 41.98 ng,respectively. RSDs of precision,stability(24 h)and reproducibility tests were all lower than 2.0％(n=6). The average recoveries were 93.0％-97.3％(RSD=1.9％,n=6) and 92.1％-97.9％(RSD=2.4％, n=6). The contents of catalpol and leonuride in 7 batches of samples were 0.53-0.81 and 0.79-1.25 mg/g, respectively. CONCLUSIONS:The established method is rapid,simple,stable and reliable,and can be used for simultaneous determination of catalpol and leonuride in C. dahurica.
ABSTRACT
OBJECTIVE: To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODS: Cymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column (4.6 mm×250 mm,5 μm) at 30 ℃ by gradient elution with CH3OH-H2O as the mobile phase, with the detection wavelength set at 210 nm.RESULTS: Under the chromatographic conditions adopted in this study, all the calibration curves exhibited good linearity (r>0.999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95.0%-98.0%, and the RSD was less than 3%. The contents of all the compounds (S1-S6) in samples from different habitats varied significantly.CONCLUSION: The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.
ABSTRACT
OBJECTIVE:To investigate the anti-inflammatory and analgesic effects of mongolian medicine Cymbaria dahurica extract. METHODS:96 KM mice(or SD rats)were randomly divided into model group(water),positive control group(aspirin, 0.5 g/kg),C. dahurica ethanol extract (70% ethanol) low-dose,medium-dose and high-dose groups (0.325,0.650,1.300 g/kg, calculated by crude drug) and C. dahurica aqueous extract of the residue of alcohol extraction low-dose, medium-dose and high-dose groups(0.325,0.650,1.300 g/kg,calculated by crude drug). They were given relevant medicine intragastrically,once a day,for consecutive 7 d. The xylene-induced ear edema method was used to determine the degree of ear edema,and egg white-in-duced paw edema method was used to determine paw edema after inducing inflammation 1,2,4,6 h;anti-inflammatory activity of C. dahurica extract was investigated. 96 KM mice were grouped and given medicine with same method;the number of writhing within 20 min was determined by acetic acid writhing method. Another 64 KM mice were grouped with same method,with 8 mice in each group;except positive control group was given tramadol hydrochloride(0.5 g/kg)intragastrically,other groups were given relevant medicine with same method. Pain thresholds of mice were determined by hot-plate test before and after medication 30,45, 60,90 min,and analgesic effects of C. dahurica extract were investigated. RESULTS:Compared with model group,C. dahurica extract could obviously restrain the ear edema of mice and paw edema of rats 6 h after egg white-induced inflammation. Except paw edema of rats in C. dahurica aqueous extract of the residue of alcohol extraction high-dose group was decreased slightly,there was statistical significance among other groups (P<0.05 or P<0.01). C. dahurica extract decreased the number of writhing in mice within 20 min,and extended pain thresholds of mice 30,60,90 min after medication (P<0.05). CONCLUSIONS:Both C. dahurica ethanol extract and aqueous extract of the residue of alcohol extraction posses certain anti-inflammatory and analgesic effects.