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Objective: To study the quality control method of Miao medicine Inula cappa based on anti-inflammatory active ingredients. Methods: Firstly, a representative and characteristic chemical composition of effective components in I. cappa was established as an indicator component for multi-index quantitative fingerprinting. Then, the quality of I. cappa in different areas of Guizhou Province was evaluated by quantitative analysis of multiple indicators and fingerprint analysis. Results: The HPLC fingerprints and multi-index content determination methods of 35 batches of I. cappa were established. Eight of 17 common peaks of the liquid chromatographic fingerprints of I. cappa were identified. The results showed that the similarity was 0.898-0.997, eight components of which were used as indicators to determine the content of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, cynarin and luteolin, which were 0.353-3.765, 0.056-0.495, 0.086-0.526, 0.306-2.526, 0.861-7.353, 0.729-4.268, 0.052-0.424, 0.148-1.102 mg/g, respectively. Conclusion: The fingerprinting and multi-index content determination methods based on anti-inflammatory active ingredients established have high sensitivity, good accuracy, stability and reliability, which can be used for quantitative control of Miao medicine I. cappa.
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ABSTRACT The aim of this study was to provide scientific knowledge to support the use of Vernonia condensata Baker, Asteraceae, beverages for their alleged hypocholesterolemic properties by testing their action as HMG-CoA reductase inhibitors and their capacity to lower dietary cholesterol permeation. Chlorogenic acid, and other caffeoylquinic acids derivatives were identified as the main components of these beverages by LC–MS/MS. No changes in the composition were notice after the in vitro gastrointestinal digestion and no toxicity against Caco-2 and HepG2 cell lines was detected. Cholesterol permeation through Caco-2 monolayers was reduced in 37% in the presence of these herbal teas, and the caffeoylquinic acids permeated the monolayers in 30–40% of their initial amount in 6 h. HMG-CoA reductase activity was reduced with these beverages, showing an IC50 of 217 µg ml−1. It was concluded that caffeoylquinic acids, the major components, justified 98% of the enzyme inhibition measured.
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In this study, we investigated whether cynaroside, cynarin and linarin derived from Chrysanthemum indicum L. affect the secretion, production and gene expression of MUC5AC mucin in airway epithelial cells. Confluent NCI-H292 cells were pretreated with cynaroside, cynarin or linarin for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA, respectively. Effect of linarin on EGF (epidermal growth factor) - or TNF-alpha (tumor necrosis factor-alpha)-induced MUC5AC mucin gene expression and mucin protein production was also examined. The results were as follows: (1) Cynaroside and cynarin did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, linarin decreased MUC5AC mucin secretion; (2) Cynaroside did not affect PMA-induced MUC5AC mucin production and gene expresion from NCI-H292 cells. However, cynarin and linarin inhibited the production and gene expression of MUC5AC mucin; (3) Linarin also inhibited the production and gene expression of MUC5AC mucin induced by EGF- or TNF-alpha from NCI-H292 cells. These results suggest that linarin can regulate the gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.
Subject(s)
Chrysanthemum , Enzyme-Linked Immunosorbent Assay , Epidermal Growth Factor , Epithelial Cells , Gene Expression , Mucins , Necrosis , Tumor Necrosis Factor-alphaABSTRACT
OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneousn determination of nchlorogenic acid, caffeic acid, ferulic acid, isochlorogenic acid A, isochlorogenic acid C, neochlorogenic acid, and cynarin in the herbs of Xanthii Herba and Xanthii Fructus. METHODS: 50 mmol·L-1 borax-water was used as buffer solution (pH 9.54). The separation was performed on an uncoated fused silica capillary (64.5 cm×75 μm, 56 cm of effective length) maintained at 25℃ at voltage of 25 kV. The detection wavelength was 326 nm, and the sample was injected at 25 kPa×6 s. RESULTS: The calibration curves of the seven phenolic acid showed good linearity (r>0.9994) in the ranges of the tested concentrations, and the average recoveries of the method were between 99.85%-102.70%. CONCLUSION: The method is simple, accurate and reproducible, and can be used for the quality evaluation and control of Xanthii Herba and Xanthii Fructus.
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Objective: To optimize the technological parameters of separation and purification of cynarin from Cynara scolymus leaves. Methods: Seven different types of macroporous adsorption resins were evaluated on absorptive capacity, desorption rate, and adsorption rate in order to select the best resin and the conditions of the best resin to separate and purify cynarin were researched. Results: It was found that LSA-21 resin showed better comprehensive adsorption property, flow rate of sample loading was 2 BV/h, 3 BV/h 50% alcohol aqueous and 2 BV/h velocity was used to elute. In this process to obtain the product with purity of 5.63% cynarin, ash content of 0.61%, product yield 5.56%, the product quality could meet the market demand. Conclusion: The LSA-21 macroporous adsorption resin shows better comprehensive adsorption property. It could be used to isolate and purify the cynarin.