ABSTRACT
In the present study the in vitro antimicrobial activity, along with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), of different extracts of leaves of Mussaenda roxburghii Hook. f. were evaluated against 13 pathogenic microorganisms. The methanol extract and its carbon tetrachloride and chloroform soluble fractions showed the highest antimicrobial activity. The chloroform soluble fraction showed the maximum inhibition zone of 16.0 mm against Bacillus megaterium with MIC and MBC values of 7.81 ug/ml and 250 ug/ml, respectively. Ciprofloxacin (30 ug/disc) was used as standard antimicrobial agent. In the brine shrimp lethality bioassay, both the petroleum-ether and carbon tetrachloride soluble fraction of crude methanol extract demonstrated strong cytotoxic activity with LC50 value of 0.52 and 0.62 ug/ml, respectively compared to that of 0.451 ug/ml exhibited by standard vincristine sulfate.
En el presente estudio, la actividad antimicrobiana in vitro, junto con la concentración inhibitoria mínima (CIM) y la concentración bactericida mínima (CBM), de diferentes extractos de las hojas de Mussaenda roxburghii Hook. f. fueron evaluadas contra 13 microorganismos patógenos. El extracto metanólico y sus fracciones solubles en tetracloruro de carbono y cloroformo mostraron la actividad antimicrobiana más alta. La fracción soluble en cloroformo mostró la zona de inhibición máxima de 16,0 mm en contra de Bacillus megaterium, con valores de MIC y CBM de 7,81 g/ml y 250 mg/ml, respectivamente. Ciprofloxacina (30 ug/disco) se usó como agente antimicrobiano estándar. En el bioensayo de letalidad con Artemia salina, tanto el petróleo-éter y tetracloruro de carbono como la fracción soluble del extracto de metanol crudo demostraron una fuerte actividad citotóxica con valores de LC50 de 0,52 y 0,62 mg/ml, respectivamente, en comparación con la de 0,451 mg/ml de sulfato de vincristina utilizado como estándar.
Subject(s)
Anti-Bacterial Agents/pharmacology , Plant Extracts/pharmacology , Rubiaceae/chemistry , Gram-Negative Bacteria , Gram-Positive Bacteria , Plant Leaves/chemistry , Methanol , Microbial Sensitivity Tests , Toxicity TestsABSTRACT
In the present study the in vitro antimicrobial activity, along with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), of different extracts of leaves of Mesua nagassarium were evaluated against 13 pathogenic microorganisms. The methanol extract and its pet-ether and carbon tetrachloride soluble fractions showed the highest antimicrobial activity. The carbon tetrachloride soluble fraction showed the maximum inhibition zone of 24.33 mm against Bacillus megaterium with MIC and MBC values of 7.81 ug/ml and 250 ug/ml, respectively. Ciprofloxacin (30 ug/disc) was used as standard antimicrobial agent. In the Brine shrimp lethality bioassay, the crude methanol extract and its carbon tetrachloride soluble fraction showed significant cytotoxicity with LC50 of 2.99 and 1.74 ug/ml, respectively as compared vincristine sulphate (LC50 value 0.543 ug/ml).
En el presente estudio se evaluó la actividad antimicrobiana in vitro, incluyendo la concentración inhibitoria mínima (CIM) y la concentración bactericida mínima (CBM), de diferentes extractos obtenidos de hojas de Mesua nagassarium en 13 microorganismos patógenos. El extracto metanólico y sus fracciones solubles en éter de petróleo y tetracloruro de carbono, mostraron la mayor actividad antimicrobiana. La fracción de compuestos solubles en tetracloruro de carbono mostró la zona de inhibición máxima de 24.33 mm en Bacillus megaterium con valores de CIM y and CBM de 7.81 ug/ml y 250 ug/ml, respectivamente. Como agente antimicrobiano estándar se utilizó ciprofloxacina (30 ug/disco). En el bioensayo de mortalidad de Brine shrimp el extracto metanólico y su fracción soluble en tetracloruro de carbono mostraron importante citotoxicidad con CL50 de 2.99 y 1.74 ug/ml, respectivamente, comparadas con el sulfato de vincristina (CL50 0.543 ug/ml).
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria , Plant Extracts/pharmacology , Ferns/chemistry , Plant Leaves/chemistry , Anti-Bacterial Agents/toxicity , Biological Assay , Bacillus megaterium , Plant Extracts/toxicityABSTRACT
Background and purpose:Dendritic cells(DCs) possess specialized feature such as pathogen recognition,antigen capturing and processing machinery,and stimulating naive T lymphocyte to have antitumor ability that allow them to act like professional APCs.This paper is aimed to confirm the impacts on the proliferation and secretion of INF-? of tumor specific CTL and its cytotoxocity induced by DC loaded with different antigen.Methods:After the stimulation of DC loaded with different antigen,the proliferative rate of allolymphocytes was measured by MTT and the cytotoxocity of CTL was evaluated with LDH method.The INF-? secreted by activated T lymphocytes was detected by ELISPOT.Results:The DC loaded with CPP-Id(320%?15%) had significantly induce T lymphocyte proliferating when comparing with the induction by DC loaded with Id(57%?10%)(P
ABSTRACT
Objective To study the impact on the proliferation and cytotoxicity of donor T cells during mobilization with rhG - CSF. Methods The peripheral blood mononuclear cells (PBMNC) were collected from 20 healthy donors before mobilization and on the fifth day of mobilization with rhG - CSF. After the PBMNC was activated with 500 ng/ml of CD3 monoclonal antibody and 500 ?g/ml of rhIL -2 for 96 hours,the activated T cells were collected for testing proliferation, cytotoxicity. Fas expression, perforin and Fas ligand (FasL) mRNA expression,and IFN -? concentration in the culture medium of the activated T cells, were determined by radioimmunoassay. Results The proliferation activity and cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL - 2 were reduced markedly after mobilization with rhG - CSF(P0.05). The activated T cells expressed perforin mRNA and didn' t express FasL mRNA both before and after mobilization with rhG - CSF. The concentration of IFN -? in the culture medium of the activated T cells decreased significantly after mobilization with rhG - CSF(P