ABSTRACT
Aims: Several chelating agents are presently used among environmental physicians to diagnose and treat a chronic metal overexposure. We evaluated and compared the binding capacity of the most common chelating agents DMPS (2, 3-dimercapto-1- propanesulfonic acid), DMSA (dimercaptosuccinic acid), also called Succimer) and EDTA (ethylene diamine tetraacetic acid) for the potentially toxic metals Antimony (Sb), Arsenic (As), Cadmium (Cd), Lead (Pb) and Mercury (Hg). Secondly, we evaluated how the nutrient elements Calcium (Ca), Copper (Cu) and Zinc (Zn) are affected by the chelating agents tested. Study Design: Through ICP-MS (Inductively Coupled Plasma Mass Spectroscopy) analysis of urine from environmentally burdened patients, we determined which chelating agent in oral or injectable form has the best potential to be used as a provocation test for the diagnosis of multiple metal over exposure, and which chelating agent is best used for the detoxification treatment of a single metal exposure. Place and Duration of Study: Micro Trace Minerals and Friedle Laboratories, Hersbruck/Regensburg, Germany, between January 2011 and February 2013. Methodology: Data utilized is based on urine samples from chronically exposed patients, male and female adults, received from chelation therapists. Acutely intoxicated patients were not included. Results: The intravenous application of DMPS is most suitable for the diagnosis and treatment of a single or multiple metal exposure, involving the metals Sb, As and Hg. Both EDTAs (NaCaEDTA and NaEDTA), administered intravenously, are the agents of choice for Cd, while Pb can be chelated using DMSA, DMPS, or the EDTAs. Both EDTAs have a strong Zn binding ability, but only NaEDTA is suitable for binding appreciable amounts of Ca. DMPS best binds Cu. Conclusion: The intravenous application of DMPS is most useful for the diagnosis of multiple metal overexposure. It is also the treatment of choice for Sb, As and Hg and has the strongest Cu binding ability of the chelators tested.
ABSTRACT
Objective To study the effects of DMPS on IL-1? during experimental myocardial ischemia-reperfusion(I/R)injury.Methods 30 New Zealand rabbits were randomly assigned to 3 groups:I/R group,DMPS protection group and Control group,10 in each group.The blood samples was obtained through vien at different time(5 min before ischemia,the end of the ischemia period and 0.5h,1h,2h,4h,6h after reperfusion)in each group.The serum concentrations of IL-1? were detected with radioimmunology method.Cardiac tissues samples were taken for determination of IL-1?.The ultrastructure changes of the Cardiac tissues were observed.Results The levels of IL-1? of serum and cardiac tissues increased after ischemia and reperfusion,and were significant different comparing with that before ischemia(P
ABSTRACT
Objective To study the effects of DMPS on ET-1 during experimental myocardial ischemia-reperfusion (I/R) injury.Methods 20 New Zealand rabbits were randomly assigned to 2 groups: I/R group and DMPS protection group with 10 in each group. The blood sample was obtained through vien at different time (5 min before ischemia, the end of the ischemia period and 0.5h, 1h, 2h, 4h, 6h after reperfusion ) in each group.The serum concentrations of ET-1 were detected with radioimmunology method. Results The levels of ET-1 of serum and cardiac tissues increased after ischemia and reperfusion, and were significant different compared with that before ischemia(P0.05).Conclusions The changes of ET-1 were significant when myocardial I/R. DMPS may effectively effect the levels of ET-1 after myocardial ischemia and during I/R injury,and have protecfion of myocardium from ischemia and reperfusion injury.
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In peroneal nerve-anterior tibialis muscle preparations of uretbane anesthetized rabbits, sodium ammonium dimethyl-2 -( propano-1 , 3 - dithiosulfonate ) monohydrate ( SCD ) 7 .5mg/kg iv depressed the antogenous respiration completely and the indirectly elicited twitch tension completely finally. Sodium 2, 3 - dimercaptopropane- 1-sulfonate ( DMPS ) 7、 10mg/kg iv,respectively completely antagonized the respiratory depression and the neuromuscle block, DMPS (2.4、 3.4、 4.9mg/kg, iv ) could antagonize these toxic effects partly. DMPS (62.5mg/kg ,iv)could antagonize the toxic symptom induced by SCD(7.5 mg/kg, iv)in rabbits. At dose level higher than 12.5mg/kg of SCD, this toxin caused tremor and tonic convulsion. e Wfound DMPS (62.5 mg/kg, iv ) combined with diazepam ( 5mg/kg, iv ) has antidotal effects on acute poisoning caused by SCD in rabbits.
ABSTRACT
In this study, we observed the efTects of systemic poisoning by sulphur mustard (SM) on DNA biosynthesis of internal organs and protective action of antidotes in mice.The results showed that the systemic poisoning by SM produced a strong depression of [3H]-TdR incorporation into DNA biosynthesis, which was characterized by rapidity, severity and rapid recovery.It is suggested that mammal an organism has a marked physiological compensation, regeneration and repair activity for DNA damage.Antidote sodium thiosufate alone or in combination with unithiol (DMPS) has satisfactory protective effects on depression of DNA biosynthesis in mice poisoned by SM.