ABSTRACT
Pancreatic cancer is a kind of malignant tumor with a high degree of malignancy and a very poor prognosis.The onset is occult,early metastasis is easy,and progression is rapid.Despite clinically increasing the resection rate of pancreatic cancer,the survival time after surgery is not significantly prolonged.Pancreatic cancer is not sensitive to radiotherapy and chemotherapy,so there is no effective treatment.Adoptive immunotherapy is an important auxiliary method for the treatment of malignant tumors and refers to killing the malignant tumor cells in patients by injecting effective immune cells into the patient without damaging the patient's own immune system.The DNT cells discovered in recent years,namely TCR +,CD3 +,CD4-,and CD8-T cells,are T-cell subpopulations with unique immunomodulatory functions,and their expression of Fas/FasL,TNF/TNFR,and MHCI,etc.They have an important role in anti-tumor,immune regulation,immunosuppression,etc.
ABSTRACT
Double negative T cells,namely DNT cells,are specific regulatory cells with TCR+ CD3 + CD4-CD8-in cell phenotype,divide into αβ T cell and γδ T cell,play a very important role in regulating immunoreaction.Previous research restricted to the treatment of autoimmune disease and organ transplantation immunity.Recently dnt cells adoptive immunotherapy,as a new anti-tumor method,gradually come into public view.This review mainly focuses on the source of DNT cells,their anti-tumor effect,as well as the possible mechanism.
ABSTRACT
Objective The immune magnetic separation(MACS)method of DNT cells was set up and their content Was detected in peripheral blood of healthy man.Methods DNT cells were separated from the peripheral blood.Trypan blue staining and flow cytometry were used to detect their activity and purity,and flow cytometry Was used to detect their content in normal human peripheral blood.Results DNT cell activi ty using MACS sorting was＞97%,the purity Was 82.77%;DNT cells accounted for(6.25±2.61)% (n=45)in the TCRαβ+T cells.Conclusion MACS Can quickly sort out high purity DNT cells,and do not affect the vitality of cells.DNT cells accounted for(6.25±2.61)%in the TCRCD3+T cells.
ABSTRACT
DNT cells(CD4-CD8-double-negative T cells)ale newly discovered immune cells,whose surface ex press neither CD4 nor CD8 molecules.In recent years,the prognosis and survival quality of tumour patient have been greatly improved through cell immunotherapy.Immunocyte can kill the tumour directly Or through immunoregu lation.The study of DNT cells(double-nnegative T cell)on cancer cells will be of vital significance.
ABSTRACT
In recent years,the prognosis and survival quality of tumour patient would be improved greatly through cell immunotherapy,which becomes the priority scientific research.Immunoeytes can kill the tumour cell directly or through immunoregulation.CD4CD8 double-negative T cells is a newly found subset of T cells whose SUlface express neither CD4 nor CD8.This article mainly introduces the advancement of one subet of DNT cell,DN Tregs,in tumor.