ABSTRACT
Aim To investigate the preventive and therapeutic effects of Danzhijiangtang capsule (DZJT) on diabetic nephropathy in rats and its mechanism. Methods Twelve rats were randomly selected as the normal group from 100 male SD rats, the remaining was induced by high fat diet and streptozotocin (34 mg • kg-1, ip), and 65 successful modeling rats were randomly divided into model group, DZJT high, medium, low dose group and pioglitazone group. Gavage was administered for 8 weeks. The blood glucose, renal function and the expression of TGF-β1 in serum were measured. The pathological changes of renal tissue were observed. The expressions of TGF-β1 Smad2, Smad3, Smad7 and CTGF in renal tissues were detected. Results Compared with normal group, the blood glucose of rats in model group significantly increased, renal and glomerular hypertrophy, basement membrane thickening, extracellular matrix increased, and inflammatory cell infiltration, fibrous tissue hyper plasia, kidney pathological score increased significantly; the expression of TGF-β1, SmacB and CTGF in renal tissues increased significantly, and the expression of Smad2 and Smad7 decreased markedly. Compared with model group, the above indicators in each treatment group decreased in different degrees, and the pathological morphology of kidney was improved obviously; TGF-β1, Smad3 and CTGF decreased, while the levels of Smad2 and Smad7 increased. Conclusions The capsule can effectively improve the symptoms of diabetes and alleviate the kidney damage in rats, and its mechanism may be related to the regulation of TGF-β1/Smads signaling pathway and its downstream CTGF expression.
ABSTRACT
Objective To investigate the effect of Danzhijiangtang capsule on serum,fat in type 2 diabetic rats.Methods The model of type 2 diabetes mellitus rats were established by injecting small does streptozotocin(STZ)into abdomen and feeding high sucrose-fat diet.All the rats were divided randomly into five groups.The level of blood glucose,serum insulin,insulin sensitivity index,blood fat(triglyceride, cholesterol,high-density lipoprotein,low-density lipoprotein),free fatty acid after treated were examined. Results Danzhijiangtang capsule could decrease the level of blood fat.Conclusion Danzhijiangtang capsule could improve the blood fat in type 2 diabetic rats.
ABSTRACT
Objective To investigate the effect of Danzhijiangtang Capsule on serum resistin level in insulin resistance (IR) rats induced by low dose of streptozotocin (STZ) and high calorie food. Methods Male Wistar rats were randomly divided into normal group, model group, low dose and high dose of Danzhijiangtang capsule treating group. The rats of normal group were fed with common food while the others with high calorie food and injected with low dose of STZ. The weight, fasting blood glucose (FBG), fasting insulin (FIns), blood fat, resistin level of all rats were measured, and insulin sensitivity index (ISI) was calculated accordingly. Results Danzhijiangtang capsule could reduce body weight of IR rats. According to the results of biochemical indicator detection, Danzhijiangtang capsule could reduce FBG and FIns level, improve blood fat condition, enhance ISI effectively and decrease resistin level (P