ABSTRACT
Objective To observe the day-time variation of serum CD28 in asthmatic adults treated with ICS and adults without asthma. Methods Serum CD28 level were measured at 3-hour intervals from 8: 00 to 17: 00 h in 82 adult participants with asthma and in 40 healthy participants without asthma during Jan 2015 to Jan 2017. CD28 levels at each timepoints of asthma patients before and after ICS therapy and healthy participants were compared by repeated measures analysis of variance. Results The level of CD28 of asthma patients before ICS were significantly higher than heathy control (F1, 120 = 232. 1, P = 0. 000). The CD28 lever of asthma patients before ICS was highest at 8: 00h and continuously reduced during the day. After ICS therapy, the level of CD28 were significantly decreased, and were significantly higher than healthy control group at 8: 00h (t = 4. 59, P ＜ 0. 01) and 11: 00h (t = 3. 51, P ＜ 0. 01). Conclusion The level of serum CD28 changed significantly and rhythmically in asthma patients, which may be a potential marker or therapeutic target in the diagnosis or therapy of asthma.