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OBJECTIVE To study the repair effect and mechanism of Zhixu burn ointment on deep second-degree burned model rats. METHODS The deep second-degree burned model was established with a temperature-controlled apparatus. The model rats were randomly divided into model group， Jingwanhong ointment group and Zhixu burn ointment high-dose， medium-dose and low-dose groups （specifications were 20 g core+20 mL wetting agent， 10 g core+20 mL wetting agent， 5 g core+20 mL wetting agent， respectively）. Another blank control group （only dehairing treatment， no modeling） was set up， with 10 rats in each group. The rats in each administration group were given corresponding drugs， the rats in the blank control group were not treated， and the rats in the model group were given normal saline once a day for 21 d. The healing of burn wounds and histomorphological changes of burned skin in each group of rats were observed， and the healing rate of wounds was calculated； the levels of vascular endothelial growth factor （VEGF）， interleukin-1β （IL-1β）， IL-6 and tumor necrosis factor-α（TNF-α） in the wound skin tissue of rats were detected by enzyme-linked immunosorbent assay； immunohistochemical method was used to detect the expression levels of VEGF and platelet endothelial cell adhesion molecule-1 （CD31） protein in burned skin tissue. RESULTS Compared with model group， the wound area of the rats in the Jingwanhong ointment and Zhixu burn ointment groups gradually decreased and healed significantly by day 21， and the wound healing rate was significantly higher （P＜0.05 or P＜0.01）； thicker new epidermal layer was seen in the skin tissue， and connective tissue and new blood vessels were significantly increased in the dermis； the expression levels of IL-1β， IL-6 and TNF-α were significantly decreased （P＜0.05 or P＜0.01）， and the expression levels of VEGF and CD31 protein were significantly increased （P＜0.05 or P＜0.01） in the burned skin tissue. CONCLUSIONS Zhixu burn ointment can repair the skin of deep second-degree burned model rats， and the mechanism may be related to reducing the inflammatory response of burn wound and promoting the angiogenesis.
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BACKGROUND: There are various methods for the treatment and repair of deep second-degree burn wounds, which have diverse effects and have no uniform standards. Therefore, new technologies need to be developed. OBJECTIVE: To observe the therapeutic effect of controlled debridement and traditional treatment on deep second-degree burn wounds. METHODS: A total of 80 patients with deep second-degree burns from June 2015 to June 2018 were enrolled and divided into a positive control group and a controlled debridement group, with 40 patients in each group. The positive control group was coated with Bangerkang burn bacteriostatic cream, and the oil gauze was pressure-wrapped. The dressing was changed at 3, 6, 9, and 12 days after the injury. In the controlled debridement group, epidermal cell suspension was prepared according to the requirements of wound preparation and was sprayed onto the wound surface. The wound was then covered with Recell Kit self-contained protective film. The film was removed to keep the wound dry on the 3rd day after operation, and then the wound was observed at 6, 9, and 12 days after operation. The study protocol was approved by the Ethics Committee of the Xijing Hospital of the Air Force Military Medical University. All patients volunteered to participate in the study and sign an informed consent. Patient information was registered online and appropriate treatment was performed according to a random (software online) assignment. RESULTS AND CONCLUSION: At 3, 6, 9, and 12 days after operation, the bacterial content, wound pain score, wound infection score and pro-inflammatory factor level in the controlled debridement group were significantly lower than those in the positive control group (P < 0.05). Moreover, there was no complication in both groups. These findings reveal that epidermal cell implantation combined with controlled debridement for deep second-degree burn wounds can achieve remarkable outcomes, which can significantly accelerate wound healing, reduce infection and alleviate the suffering of patients.
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PURPOSE: Aim of this study was to assess the safety and effectiveness of Keraheal-Allo® (Biosolution Co., Ltd., Korea) in patients with deep second-degree burn as a part of post marketing surveillance. METHODS: Seventy-five patients with deep second-degree burn were enrolled from April 2017 to October 2017. Keraheal-Allo, a thermos-sensitive hydrogel-type allogeneic keratinocytes, was applied to 90 deep second-degree burn sites of 75 patients. After application of Keraheal-Allo, the efficacy was assessed as the period of 100% re-epithelialization that was evaluated every time dressing was changed. RESULTS: The mean re-epithelialization period in the treated sites with KeraHeal-allo was 13.67±5.11 days. There was no severe adverse event. CONCLUSION: In conclusion, this thermo-sensitive hydrogel-type allogeneic keratinocytes have the clinical usefulness in terms of the safety, efficacy and ease of use.
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Humans , Bandages , Burns , Keratinocytes , Marketing , Re-EpithelializationABSTRACT
OBJECTIVE: To prepare a hydrogel dressing and investigate its effect on the healing of deep second degree burn wounds in SD rats. METHODS: A hydrogel dressing was prepared and its sterilization method was screened. The skin irritation of the hydrogel dressing was investigated in New Zealand rabbits. A deep second degree burn model of SD rats was prepared by hot solid scald method. Tuojia ointment and Intrasite Gel were adopted as positive controls to investigate its effect on the wound healing. RESULTS The prepared hydrogel dressing was clear and transparent, and the viscosity was well consistent with Intrasite Gel. After radiation sterilization, the viscosity of the hydrogel was sharply decreased. However, the viscosity was not significantly changed before and after high pressure steam sterilization. The hydrogel had no significant skin irritation. The healing time of the experimental group was not significantly shorten than that of the positive control, but has significant difference compared with the vehicle control group(P<0.01). CONCLUSION: Hydroxyethyl cellulose can be used as the matrix of hydrogel dressing. The prepared hydrogel dressing has appropriate viscosity, clarification, and no obvious skin irritation. Besides, it has remarkable healing effect on deep second degree burn wounds in SD rats. So it may have broad application prospects.
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Objective:To evaluate the efficacy and safety of recombinant human acidic fibroblast growth factor( rh-aFGF)and re-combinant human basic fibroblast growth factor( rh-bFGF)in the treatment of deep second degree burn. Methods:A multicenter,pro-spective,randomized and double-blind clinical trial was conducted. Totally 216 cases of deep second degree burn were selected from five research centers and given appropriate antibiotics and nutritional supplement therapy. The wound of 108 cases in the observation group were rinsing with rh-aFGF(one bottle / 5cm2)according to the wound area at the time of admission followed by rh-aFGF daily spraying,3-4 press/cm2 ,6-8 times a day. The 108 patients in the control group were treated with rh-bFGF with the same regimen as the observation group. After the 30-day follow-up,the wound healing was evaluated in the two groups. Results:The complete healing time,debridement time,complete healing rate in 12 days and 15 days in the observation group were all better than those in the control group(P<0. 05). After the 7-day treatment,the level of leukocyte and seepage score of the observation group were both lower than those of the control group(P<0. 01). The moderate rate showed significant difference between the two groups(P<0. 05). Conclu-sion:rh-aFGF shows better clinical efficacy than rh-bFGF in the treatment of deep second degree burn with the similar safety.
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PURPOSE: This study was planned to evaluate the effect of plant extracts contained dressing material which is rich in procyanidins for treatment of deep second degree burn. METHODS: This study conducted from September 1, 2013 to February 28, 2014. Patients with the deep dermal burn wound which is larger than 200 cm2 were enrolled in this study. The test material was applied total 3 times every 2 days when the wounds were relatively clean, with thin eschar and scab removed and the dermal layer exposed. The test and control sites were treated with hydro-foam equally. The effectiveness was evaluated by comparing the re-epithelialization rates between the test and control sites. Two burn surgeons blindly evaluated for re-epithelialization. Evaluation of adverse reaction was also performed during study period. RESULTS: Total of 40 patients were enrolled. Mean total body surface area burned% was 12.3+/-5.5% and the test material was first applied at mean post burn day # 7.2+/-2.0.The re-epithelialization period was 9.0+/-1.7 days in the test site and 11.1+/-2.0 days in the control site. In the test site, re-epithelialization was 2.1+/-1.0 days faster than in the control site (P<0.0001). There was no significant adverse reaction during study period. CONCLUSION: The plant extracts contained dressing material which is rich in procyanidins accelerates wound healing time and shows the safety.