ABSTRACT
Objective To investigate the effects of dehydrocostuslactone(Dehy) on human hepatic stellate LX-2 cells proliferation,apoptosis and the possible related mechanisms.Methods After treating the LX-2 cells by different concentrations of Dehy,the MTT assay and flow cytometry were used to assess the influence of Dehy on cell proliferation and cycle distribution of LX-2 cells.The Hoechst 33342 staining and AV-PI dual staining were used to detect the effect of Dehy on cell apoptosis in LX-2 cells.The apoptosis related proteins expression was detected by Western blot.Results After different concentrations of Dehy acting for 48 h could significantly inhibit the proliferation of LX-2 cells,blocked the cellular cycle at stage S and G2/M,meanwhile induced LX-2 cells apoptosis in the concentration dependent manner;Western blot results showed that Dehy could promote the up-regulation of P27 and bax expression and down-regulation of Bcl-2.Conclusion Dehy induces human liver LX-2 cells apoptosis and cell cycle arrest by adjusting the expression of Bax,Bcl-2 and P27.
ABSTRACT
Objective To investigate the effects of dehydrocostuslactone(Dehy) on human hepatic stellate LX-2 cells proliferation,apoptosis and the possible related mechanisms.Methods After treating the LX-2 cells by different concentrations of Dehy,the MTT assay and flow cytometry were used to assess the influence of Dehy on cell proliferation and cycle distribution of LX-2 cells.The Hoechst 33342 staining and AV-PI dual staining were used to detect the effect of Dehy on cell apoptosis in LX-2 cells.The apoptosis related proteins expression was detected by Western blot.Results After different concentrations of Dehy acting for 48 h could significantly inhibit the proliferation of LX-2 cells,blocked the cellular cycle at stage S and G2/M,meanwhile induced LX-2 cells apoptosis in the concentration dependent manner;Western blot results showed that Dehy could promote the up-regulation of P27 and bax expression and down-regulation of Bcl-2.Conclusion Dehy induces human liver LX-2 cells apoptosis and cell cycle arrest by adjusting the expression of Bax,Bcl-2 and P27.
ABSTRACT
Objective:To analyze and evaluate the quality control of Radix Aucklandiae in Xianglian preparation and develop a new quality control method for the preparation through standard testing and exploration study on 127 batches of samples .Methods:Ac-cording to the mandatory method , Radix Aucklandiae was identified by TLC .In the exploratory research , the volatile compounds in Xianglian preparation was analyzed by SPME/GC-MS, and the GC-MS fingerprints of volatile compounds in Xianglian preparation with different dosage forms were established .The contents of costunolide and dehydrocostuslactone in Xianglian preparation were determined by UPLC.Results:According to the current quality standard , all the batches of samples met the standard with the qualified rate of 100%.The composition of volatile compounds in different dosage forms was similar , while the relative contents were quite different . The contents of costunolide and dehydrocostuslactone in Xianglian preparation were different .Conclusion: The exploratory research provides reference for the further revision of drug standard and monitoring quality of the preparation .
ABSTRACT
Objective: To establish an HPLC method for the determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. Methods: The determination was performed on VP-ODS (250 mm × 4.6 mm, 5 μm) with column temperature set at 25℃. The mobile phase consisted of methanol-0.4% phosphoric acid water (72∶28) at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm. Results: The linear range of costunolide and dehydrocostuslactone was 203.12-2 031.20 ng and 207.84-2 078.40 ng (r = 0.999 9), with average recovery at 101.1% and 101.2%, RSD = 0.48% and 0.73% (n = 6). Conclusion: The method proved is simple, accurate, reproducible, and specific, and it can be used for the quality control and evaluation of Liuwei Muxiang Capsules.
ABSTRACT
Objective: To analyze the absorption difference of the principal active components in Vladimiriae Radix, costunolide and dehydrocostuslactone, between the crude Vladimiriae Radix (CVR) and roasting-processed Vladimiriae Radix (RPVR), in stomach, small intestine, and colon in rats. Methods: The in situ perfusion models of stomach, small intestine, and colon in rats were established, the contents of costunolide, dehydrocostuslactone, and phenol red were determined using HPLC-UV method, and the parameters of absorption kinetics of the test object in stomach, small intestine, and colon were obtained. Results: The absorption percentage per hour of costunolide and dehydrocostuslactone in CVR was higher than that in RPVR in stomach and small intestine of rats (P < 0.05). However, the absorption percentage per hour of costunolide and dehydrocos in RPVR was higher than that in CVR in colon of rats (P < 0.01). Conclusion: The absorption rate of cstunolide and dehydrocostuslactone in CVR is higher than that in PVR sample in colon of rats.
ABSTRACT
AIM:To establish an HPLC method for determining cinnamaldehyde,costunolide and dehydrocostuslactone in the supercritical carbon dioxide extraction of Cinnamomum cassia and Aucklandia lapp.METHODS:The assay was performed on an Agilent HC-C_(18)(150 mm×4.6 mm,5 μm)column by UV detector at the wavelength of 210 nm with acetonitrile-water(gradient elutio)as the mobile phase at the flow rate of 1.0 mL/min,and the column temperature was 30℃.RESULTS:There were good relationships between peak area and sample size of cinnamaldehyde in the range of 148.5-1 732.5 ng,between peak area and sample size of costunolide in the range of 69.42-809.9 ng,and between peak area and sample size of dehydrocostuslactone in the range of 70.32 to 820.4 ng.Average recoveries of them were in turn 99.65%(RSD 0.72%)-99.57%(RSD 1.28%),and 98.90%(RSD 0.81%),respectively.CONCLUSION:The present method is convenient,sensitive and accurate with good reproducibility and can be used for the quality control of the supercritical CO_2 extract of Cinnamomum cassia and Aucklandia lapp.
ABSTRACT
OBJECTIVE:To establish an RP-HPLC method for simultaneous determination of the contents of costunolide and dehydrocostuslactone in Xuedan weichang pill. METHODS:The determination was performed on Shim-pack VP-ODS column(150 mm?4.6 mm,5 ?m) with methanol-water(60∶40) as mobile phase and the detection wavelength was set at 225 nm.RESULTS:The linear ranges of costunolide and dehydrocostuslactone were 13.6～272.0 ?g?mL-1 and 11.25～225.0 ?g?mL-1,respectively,and their average recoveries were 99.47% and 99.62%,respectively with RSD of 1.75%(n=6) and 1.14%(n=6),respectively. CONCLUSION:The method established is simple,feasible,accurate and reliable,and it is applicable for the quality control of Xuedan weichang pill.
ABSTRACT
OBJECTIVE:To establish a method for the quality control of Jiuweiniuhuang pills.METHODS:TLC was applied for the quantitative identification of Bostaurus domesticus,Carthamus tinctorius and Aucklandia lappa in this prescription as well as the testing of the limit of Aristolochic acid A. RP-HPLC method was established for determination of Costunolide and Dehydrocostuslactone.RESULTS: The TLC spots were clear,well-separated,specific and free from interference of negative sample. The content of Aristolochic acid A which stood at less than 2.5 ?g?6 g-1 was up to the standard.Costunolide showed a linear relationship at the concentration range of 0.26～1.3 ?g with an average recovery of 99.8% (RSD=1.1%,n=6); and Dehydrocostuslactone showed a linear relationship at the concentration range of 0.258～1.29 ?g with an average recovery of 98.2%(RSD=1.7%,n=6).CONCLUSION: The method is suitable for the quality control of Jiuweiniuhuang pills.
ABSTRACT
AIM: To establish a method of simultaneously determining 5 components in Muxiang Fenqi Pill(Flos Caryophylli,Radix Aucklandiae,Cortex Magnoliae Officinalis). METHODS: Five components :eugenol,(costunolide),dehydrocostuslactone,magnolol and honokiol in Muxiang Fenqi Pill were determined simultaneously by HPLC,using a Kromasil C_(18) column(250 mm?4.6 mm,5.0 ?m),acetonitrile-menthanol-water(50∶8∶42) as a mobile phase.The detection wavelength was at 210 nm. RESULTS: The relationship between the concentrations and the peak areas of eugenol,costunolide,dehydrocostuslactone,magnolol and honokiol were linear respectively.The RSD of precision,repeatability and recovery were all less than 1.5%. CONCLUSION: The method is simultaneous determination for five components in Muxiang Fenqi Pill,and can be applied to the quality control of Muxiang Fenqi Pill.