ABSTRACT
Objective To explore the effect of different factors on strengthening the tissue culture seedlings and roots of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju, so as to provide experimental basis for establishing an in-vitro rapid propagation system of Dendranthema morifolium. Methods The experiment took Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju tissue culture seedling as the studying object. By using tissue culture technology, different additives were added into the MS medium. Results In the seedling-strengthening culture medium, 150 mL/L of macro-element was beneficial to the growth of tissue culture seedlings of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju. The culture medium with 100 g/L of coconut milk added could obviously promote the growth of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju tissue culture seedlings. And activated carbon added to the rooting culture medium could induce the formation of tissue culture root system of Dendranthema morifolium ( Ramat) Tzvel. cv. The addition of α-naphthaleneacetic acid ( NAA) and indolebutyric acid ( IBA) at certain concentrations could promote the amount and the growth of the roots. Conclusion The tissue culture seedlings of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju are boosted by increasing the amount of macro-element, reducing the concentration of agar in the culture medium and adding coconut milk and growth hormones to the culture medium. The optimal rooting culture medium is a compound of 1/2MS, NAA 0.05 mg/L and IBA 0.05 mg/L.
ABSTRACT
O objetivo deste trabalho foi determinar a qualidade e mensurar a turgescência em hastes florais de crisântemos armazenadas em água potável e água destilada, utilizando o método de teor relativo de água (TRA) e o equipamento Wiltmeter® para determinação da pressão de turgescência. As hastes foram colhidas em um campo comercial de flores e acondicionadas ao acaso unitariamente em recipientes contendo água destilada ou água potável. Para acompanhar a condição hídrica das hastes, foram realizadas avaliações da variação da massa fresca, taxa de absorção de água, taxa de transpiração de água, teor relativo de água e pressão de turgescência. Os dados foram submetidos à análise de variância pelo teste F e as médias comparadas ao longo dos dias de vida de vaso, utilizando-se o teste de Tukey a 5 por cento de significância. Para comparação do TRA e a pressão de turgescência, foi calculado o coeficiente de correlação de Pearson. As hastes mantidas em água potável obtiveram melhor resultado na manutenção da turgescência. Há correlação entre as avaliações de pressão de turgescência e o teor relativo de água, que se mantêm mais altas à medida que os tecidos vegetais estão novos e bem hidratados. A estimativa da pressão de turgescência foi eficiente e sensível na avaliação da condição hídrica dos tecidos das hastes de crisântemos cortados.
The main goal of this research was to determine the quality by measuring turgor in flowers stems of chrysanthemums stored in tap water and on distilled water, using the method of relative water content (RWC) and the equipment Wiltmeter®. The flower stems were harvested in a commercial field and placed randomly into unitary containers containing distilled water or tap water. In order to monitor the water status of the stems the follow analyses were done: variation of fresh mass, water absorption rate, water transpiration rate, relative water content and turgor pressure. Data were subjected to analysis of variance by F test, means were compared through the days of vase life, using Tukey test at 5 percent significance level. For comparison of the RWC and the turgor pressure the Pearson correlation coefficient was calculated. Flower stems kept in tap water maintained turgor significantly better than the ones maintained on distilled water. There is a correlation between turgor pressure and relative water content, which remain higher as the plant tissues are new and well hydrated. The estimate of the turgor pressure was effective and sensitive in evaluating the water status of the tissues of the stems of cut chrysanthemums.
ABSTRACT
AIM: To investigate the effect of Dendranthema morifolium (Ramat) Tzvel (DM) on isolated rat heart and ventricular myocytes during ischemia/anoxia and reperfusion/reoxygenation.METHODS: The Langendorff perfused rat hearts were used to measure intraventricular pressure and coronary flow. The cell contraction and intracellular calcium transient in enzymatically isolated ventricular myocytes were determined. RESULTS: (1) DM (0.5 g/L) significantly attenuated the inhibitory effects induced by ischemia/reperfusion on left ventricular developed pressure (LVDP), ?dp/dt max, coronary flow and LVDP?HR, meanwhile increased the content of SOD and decreased the content of MDA in the myocardium; (2) DM (0.5 g/L) attenuated the inhibitory effects of anoxia and reoxygenation on [Ca 2+]i transient and cell contraction in isolated ventricular myocytes. CONCLUSION: DM attenuated the effects on contractility and intracellular calcium induced by ischemia/anoxia and reperfusion/reoxygenation in the isolated rat heart and the ventricular myocytes. The mechanism might be related to increase in SOD activity and maintaining [Ca 2+]i homeostasis.