ABSTRACT
OBJECTIVE@#To study evolutionary relationship of the 5'untranslated regions (5'UTRs) in low passage dengue3 viruses (DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution (1977-2000) comparing to the DEN3 prototype (H87).@*METHODS@#The 5'UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and sequenced. Their multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD software. Replication of five Thai DEN3 candidates comparing to the H87 prototype were done in human (HepG2) and the mosquito (C6/36) cell lines.@*RESULTS@#Among these Thai DEN3, the completely identical sequences of their first 89 nucleotides, their high-order secondary structure of 5'UTRs and three SNPs including the predominant C90T, and two minor SNPs including A109G and A112G were found. The C90T of Thai DEN3, Bangkok isolates was shown predominantly before 1977. Five Thai DEN3 candidates with the predominant C90T were shown to replicate in human (HepG2) and the mosquito (C6/36) cell lines better than the H87 prototype. However, their highly conserved sequences as well as SNPs of the 5'UTR did not appear to correlate with their disease severity in human.@*CONCLUSIONS@#Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence, the high-order secondary structure and the predominant C90T of the 5'UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3.
ABSTRACT
Objective:To study evolutionary relationship of the 5’untranslated regions (5’UTRs) in low passage dengue3 viruses (DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution (1977-2000) comparing to the DEN3 prototype (H87).Methods:The 5’UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and sequenced. Their multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD software. Replication of five Thai DEN3 candidates comparing to the H87 prototype were done in human (HepG2) and the mosquito (C6/36) cell lines.Results:Among these Thai DEN3, the completely identical sequences of their first 89 nucleotides, their high-order secondary structure of 5’UTRs and three SNPs including the predominant C90T, and two minor SNPs including A109G and A112G were found. The C90T of Thai DEN3, Bangkok isolates was shown predominantly before 1977. Five Thai DEN3 candidates with the predominant C90T were shown to replicate in human (HepG2) and the mosquito (C6/36) cell lines better than the H87 prototype. However, their highly conserved sequences as well as SNPs of the 5’UTR did not appear to correlate with their disease severity in human.Conclusions:Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence, the high-order secondary structure and the predominant C90T of the 5’UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3.
ABSTRACT
Objective: To study evolutionary relationship of the 5'untranslated regions (5'UTRs) in low passage dengue3 viruses (DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution (1977-2000) comparing to the DEN3 prototype (H87). Methods: The 5'UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and sequenced. Their multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD software. Replication of five Thai DEN3 candidates comparing to the H87 prototype were done in human (HepG2) and the mosquito (C6/36) cell lines. Results: Among these Thai DEN3, the completely identical sequences of their first 89 nucleotides, their high-order secondary structure of 5'UTRs and three SNPs including the predominant C90T, and two minor SNPs including A109G and A112G were found. The C90T of Thai DEN3, Bangkok isolates was shown predominantly before 1977. Five Thai DEN3 candidates with the predominant C90T were shown to replicate in human (HepG2) and the mosquito (C6/36) cell lines better than the H87 prototype. However, their highly conserved sequences as well as SNPs of the 5'UTR did not appear to correlate with their disease severity in human. Conclusions: Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence, the high-order secondary structure and the predominant C90T of the 5'UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3.
ABSTRACT
El dengue amenaza hoy en el mundo a más de 2 500 millones de personas y más de 100 países. A pesar de afectar de manera especial a la población femenina en su periodo gestacional, no se cuenta con suficientes estudios que aborden su repercusión sobre la salud materno perinatal. OBJETIVO: Valorar la repercusión causada por el dengue serotipo 3 en el embarazo y producto de la concepción. MÉTODO: Se realizó una investigación observacional, analítica de casos y controles pareados en 30 gestantes que sufrieron la enfermedad en la provincia de Santiago de Cuba durante el brote de abril a noviembre de 2006 y que fueron confirmadas a través de IgM e IgG. Los controles se seleccionaron a razón 2:1, pareados según la maternidad donde ocurrió el parto. RESULTADOS: Se observó la presencia de amenaza de parto pretérmino en gestantes con DEN 3 en el 13,3 por ciento contra 3,6 por ciento del grupo que no enfermó. Los daños placentarios que llevan al sufrimiento fetal y al parto distócico (33,3 por ciento). En el recién nacido la restricción de su crecimiento fue de10 por ciento en los casos con DEN 3 contra el 3,6 por ciento de los que no sufrieron el dengue. CONCLUSIÓN: El dengue serotipo 3 durante el embarazo incrementa la presencia de la restricción del crecimiento fetal
Nowadays, dengue threats at world scale to more than 2 500 millions of persons and to more then 100 countries. Despite affecting in a special way to female sex during its gestational period there are enough studies approaching its repercussion on perinatal maternal health. OBJECTIVE: To assess the repercussion provoked on pregnancy and fetus by dengue 3 serotype. METHOD: A observational, analytical research of cases and controls paired in 30 pregnants suffered this disease in Santiago de Cuba province during the epidemic from April to November, 2006 was made, confirmed by IgM and IdG. Controls were selected by a 2:1 ratio, paired according to institution were labor occurred. RESULTS: There was a preterm birth threat in pregnants with dengue serotype 3 in the 13.3 percent versus 3.6 percent in healthy group. Placental damages affecting the fetus and the dystocia labor (33.3 percent). In newborn growth restriction was of 10 percent in cases con dengue serotype 3 versus 3.6 percent without this condition. CONCLUSION: Dengue serotype 3 during pregnant increases presence of fetal growth restriction
Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Pregnancy Complications/epidemiology , Pregnancy Complications/etiology , Dengue/complications , Risk Factors , Observational Studies as TopicABSTRACT
El dengue, enfermedad considerada la arbovirosis que afecta más al ser humano, ha sido poco estudiada y en especial el comportamiento clínico de esta entidad durante el embarazo, por lo que nos motivamos a describir la evolución del dengue durante el embarazo. OBJETIVO: caracterizar el comportamiento clínico de la infección por el dengue serotipo 3 en la mujer en edad fértil. MÉTODOS: se realizó una investigación descriptiva durante la infección por dengue serotipo 3, se estudiaron 31 gestantes con diagnóstico confirmado de la enfermedad y una muestra de 69 no gestantes con el mismo diagnóstico pareados por edad y área de salud, durante el brote de dengue de 2006 en Santiago de Cuba. RESULTADOS: los principales resultados encontrados fueron que no existieron diferencias entre los grupos con los antecedentes de asma y sicklemia, un mayor porcentaje para las mestizas (54,8 por cientoy 65,2 por ciento) y predominaron las formas leves de la enfermedad (80,6 por ciento y 79,7 por ciento) para embarazadas y no embarazadas respectivamente; se apreció una evolución en días más corta para todos los síntomas durante el embarazo, excepto para la diarrea (0,46 vs 0,1), el sangramiento de las encías (0,19 vs 0) y la prueba del lazo positiva (2,16 vs 1,83). CONCLUSIONES: el cuadro clínico en el embarazo, a pesar de mostrarse en algunos casos en formas graves, tuvo una evolución más benigna.
The dengue, diseased considered the arbovirus affecting more the human beings, has not much studied and specially the clinical behavior of this entity during pregnancy, thus we describe dengue course during pregnancy. OBJECTIVE: to characterize the clinical behavior of serotype 3 dengue infection; in fertile age women. METHODS: a descriptive research during serotype 3 infection dengue was performed, 31 women were studied with a confirmed diagnosis of this disease and a sample of 69 non-pregnants with similar diagnosis matched by age and health area during the dengue outbreak of 2006 in Santiago de Cuba province. RESULTS: main results achieved included: not differences among groups with backgrounds of asthma and sicklemia, a great percentage for black race (54,8 percent and 65,2 percent) and a predominance of slight forms of disease (86,6 percent and 79,7 percent) for pregnants and non-pregnants, respectively; we noted a shorter daily course for all symptoms during pregnancy, except to diarrhea (0,46 versus 0,1), gingival bleeding (0,19 versus 0), and the positive serrenoeud test (2,16 versus 1,83). CONCLUSIONS: clinical picture during pregnancy despite its presence in some cases in a severe form had a more benign course.