ABSTRACT
Objective To observe the effect of Ziyin-Jianghuo formula in treatment of Yin deficiency generating intrinsic heat type of the levels of monoamine neurotransmitters and estrogens in rats, and investigate its intervention in the neuroendocrine system. Methods There were 7 groups, which were sham operation group, model control group, estrogen tablet group, Gengnian capsule group, Ziyin-Jianghuo formula low, medium and high dose groups. Castration was performed by castration (extraction of ovaries) plus hot traditional Chinese medicine. The rats in the treatment group were given the above drugs 24 hours after the last administration of the hot Chinese medicine, once a day for 30 consecutive days. The sham operation group and the model control group were given an equal volume of purified water by simultaneous intragastric administration. The serum estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL) levels,uterine coefficient and monoamine neurotransmitters dopamine (DA), norepinephrine (NE), serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) content in the hypothalamus were detected after 12 hours of the last treatment. Results Compared with the model group, the levels of serum FSH (4.39 ± 0.22 IU/L, 2.89 ± 0.91 IU/L, 2.84 ± 0.98 IU/L vs. 5.51 ± 0.24 IU/L), LH (14.48 ± 0.24 IU/L, 11.46 ± 0.33 IU/L, 5.28 ± 1.31 IU/L vs. 15.02 ± 0.37 IU/L) in the low, middle and high doses of Ziyin-Jianghuo formula significantly decreased (P<0.05), the levels of serum E2 (39.84 ± 6.08 pmol/L, 48.65 ± 6.77 pmol/L, 64.96 ± 7.97 pmol/L vs. 33.16 ± 4.62 pmol/L) significantly increased (P<0.05). The content of DA, 5-HT, 5-HIAA in the hypothalamus and the 5-HT/NE (0.48 ± 0.02, 0.43 ± 0.03, 0.27 ± 0.02 vs. 0.67 ± 0.02), 5-HIAA/5-HT (1.74 ± 0.09, 1.71 ± 0.10, 1.80 ± 0.17 vs. 2.00 ± 0.10) in the low, middle and high doses of Ziyin-Jianghuo formula significantly decreased ( P<0.05), the content of NE (663.34 ± 9.81 ng/kg, 695.94 ± 10.54 ng/kg, 790.76 ± 16.35 ng/kg vs. 602.95 ± 13.24 ng/kg) in the hypothalamus in the low, middle and high doses of Ziyin-Jianghuo formula significantly increased (P<0.05). The levels of serum PRL (10.16 ± 1.26 μg/L, 7.22 ± 1.26 μg/L vs. 14.80 ± 1.64 μg/L) in the middle and high doses of Ziyin-Jianghuo formula significantly decreased (P<0.05). Conclusions The Ziyin-Jianghuo formula has a significant positive regulation effect on the neuroendocrine system of menopausal Yin deficiency generating intrinsic heat type of rats, and this process is dose-dependent and can improve a series of symptoms caused by autonomic dysfunction.
ABSTRACT
Objective To study the application of micro -screw implant anchorage combined with segmental arch technique on posterior teeth in elongated jaw to depress edentulous area.Methods 29 adult patients with 31 posterior teeth were depressed with micro -screw implant as skeletal anchorage combined with segmental arch tech-nique in order to help restoration.Results 31 posterior teeth in the experimental group were successfully depressed to the occlusal plane with micro -screw implant anchorage combined with segmental arch technique in order to create restoration space in edentulous area with an average course of treatment of 10 months.29 posterior teeth in the control group were grinded and given root canal therapy to create restoration space in edentulous area.Conclusion The ap-plication of micro -screw implant anchorage combined with segmental arch technique on posterior teeth in elongated jaw to depress edentulous area is an effective straightening method, whereas grinding is the last resort in creating res-toration space in edentulous area.Because patients in primary hospitals have relatively low oral health awareness, some hope for faster and easier solutions and find it difficult to accept traumatic micro -screw implant.
ABSTRACT
OBJECTIVE To study the antimicrobial activity of antimicrobial plastics and its depressing effect to biofilm on the plastics′s surface. METHODS Test tube dilution method was adopted to study the antimicrobial activity and spectrum of antimicrobial plastics. Plate live bacterial recording method and scanning electron microscope(SEM) were introduced to study the formation of biofilm. RESULTS Wide inhibiting spectrum to pathogenic microbe such as Pseudomonas aeruginosa,Staphylococcus aureus,and Escherichia coli was confirmed,and the biofilm was formed after four stages,ie deposit,adhesion,propagation and biofilm formation,and propagation of microbe was found to be inhibited greatly in the surface of antimicrobial plastics,so that the formation of biofilm in the surface of antimicrobial plastics was held back,and the observation of SEM also confirmed that no biofilm was formed in the antimicrobial plastics′s surface. CONCLUSIONS Antimicrobial plastics could inhibit pathogenic microbe remarkably with wide inhibiting spectrum,and it could also depress the formation of biofilm in the surface,which indicated that it is a promising approach to prevent catheter related infections by adopting antimicrobial catheters.
ABSTRACT
Four fractions (starches, water insoluble proteins, water soluble fractions and residue) were separated from oats by means of filtration and repeated high speed centrifugation, and the lipids were extracted by acid-hydrolysis as well. Then each group of experiment hyperlipemic mice, caused by high lipid diet, were fed with these fractions for 10 days respectively. The results showed that the effectiveness of the residue fraction which was rich in dietary celluloses and lipids was similar to the whole oats on depressing bl-ood-lipid and that the water insoluble proteins were effective too. The lip-ids, however,were important but not unique effective composition in the residue of oats.