ABSTRACT
La intoxicación por sulfato ferroso es importante en niños, provoca lesiones graves en diferentes órganos, incluso la muerte si no se trata inmediatamente, es importante mencionar que los daños ocasionados estarán en relación con la cantidad ingerida. El diagnostico se realiza principalmente con la clínica y laboratorio el cual indicara valores anormales de hierro en sangre, el tratamiento rápido con Desferoxamina, permite la fácil eliminación de hierro. La intoxicación por sulfato ferroso debe ser atendida rápida e inmediatamente, saber que hacer puede ayudar en mucho al paciente y se puede evitar las complicaciones. Se presenta el caso de un niño de 1 año con un cuadro clínico de aproximadamente 2 horas caracterizado por vómitos en varias oportunidades de color café, alteración de conciencia, posterior a la ingesta de aproximadamente 40 tabletas de sulfato ferroso. Es referido del centro de salud Sumun Paya al servicio de emergencias del Hospital pediátrico Manuel AsencioVillarroel por presentar intoxicación por sulfato ferroso, a quien se le manejo inmediatamente realizándole un lavado gástrico.
The intoxication for ferrous sulfate is important in children; it causes grievous bodily harm in different organs, even the death if it is not immediately, it is important to mention that the caused damages will be in connection with the ingested quantity. He diagnoses he/she is carried out mainly with the clinic and laboratory which indicated abnormal securities of iron in blood, the quick treatment with Desferoxamina, it allows the easy iron elimination. The intoxication for ferrous sulfate should be assisted quick and immediately, to know that to make can help in a lot to the patient and you can avoid the complications. The case of a 1 year-old boy is presented with a clinical square of approximately 2 hours characterized by vomits in several opportunities of brown color, conscientious alteration, later to the ingesta of approximately 40 ferrous sulfate pills. It is referred of the center of health Sumun Paya to the service of emergencies of the pediatric Hospital Manuel AsencioVillarroel to present intoxication for ferrous sulfate, to who is managed carrying out him a gastric laundry immediately.
ABSTRACT
Objective To investigate the possible effects and underlying mechanisms of desferroxamine (DFO) preconditioning against hypoxia in neurons. Methods Cortical neurons were cultured in DFO under ischemia condition of oxygen-glucose deprivation (OGD). Cell viability was determined by cell counting kit-8 (CCK-8) method; apoptotic cell ratio was examined with Hoechst 33342 staining; the morphological change was observed. Middle cerebral artery was occluded with or without DFO administration to establish the cerebral ischemia rat model. Infarct sizes were examined by TIC staining, and the neurological severity score was evaluated. Meanwhile immunofluorescent staining was employed to detect the protein synthesis of hypoxia inducible factor-1 (HIF-1) and erythropoietin (EPO), RT-PCR was performed to detect the mRNA expression of HIF-1 and EPO as well Results Neuronal viability kept in 49% (OGD group was 25%, t =8. 544, P<0. 05), the rate of apoptosis was 38% (OGD group was 30%, t = 4. 409, P <0.05 ) after administration of DFO (post-DFO) , the morphology of neurons improved. In the model of focal cerebral ischenfia of 30 mg/kg group, neurological severity score was reduced, the percentage of brain infarct decreased 8.5% (t=4.649, P<0.05) 3 days post-DFO(vs control). In the 100 mg/kg group, neurological severity score was 7.44 ±0.39 (t=2.903, P<0.05 ) ,5.60±0.47 (t=10.143, P < 0.01 ) ,6.97 ±0.73 (t=3.142, P<0.05 ), the percentage of brain infarct decreased 12. 0% (t=5.056, P<0.05), 32.3% (t =10.993, P<0.01), 10.6% (t =4.385, P<0.05)2,3 and7 days post-DFO(vs control), respectively. Immunofluorescent staining found synthesis of HIF-1α and EPO in cultured cortex neurons after DFO pretreated; HIF-1α and EPO were upregulated in the neurons of rat brain after DFO pretreated. The mRNA of HIF-1α and EPO upregulated in vivo and in vitro. Conclusion DFO preconditioning can protect the brain against ischemic damage, which is related to the protective effect on neurons. The mechanism of DFO preconditioning may be involved in the expression of HIF-1α and EPO in vivo and in vitro.