ABSTRACT
Objective To explore bone formation markers in dexamethasone intervention osteocalcin (OC),bone alkaline phosphatase (BAKP),and type Ⅰ original amino terminal propcptide (PINP) relationship with bone longitudinal growth.Methods The selected thirty-three 4-week-old male SpragueDawley (SD) rats were randomly divided into two groups:the dexamethasone group (n =18) and the control group (n =15).The rats in the dexamethasone group received dexamethasone (200 μg/100 g) by intraperitoneal injection for 10 days.The rats in the control group received matching volume sodium chloride solution.All rats were weighed everyday.The rats were killed by using 10％ chloral hydration at 8 AM of 11 th day.The length of tibiae was measured.The proximal tibiae were excised,fixed and decaleified.Mter paraffin embedded,sections in 5 μm thick were cut.The growth plate sections were stained by haematoxylin-eosin (HE) histochemistry method.Total height of growth plate was measured.The rats decaptitating and the blood were collected.Serum was separated and stored in-80 ℃ refrigerator for analysis.Enzyme -linked immuno sorbent assay (ELISA) method was used to detect the rat OC,BAKP and PINP values.Results The length of growth plate and tibiae of dexamethasone group were significantly decreased contrast the control group:the length of growth plate (P =0.001),and the length of tibiae (P =0.000).There were no significant differences between two groups of the value of OC,BAKP and PINP:OC (P =0.056),BAKP (P=0.122),and PINP (P =0.169).There was positive correlation between the serum OC and the length of tibiae (r =0.454,P =0.08) in control group,the PINP and OC (r =0.521,P =0.026) in dexamethasone group.Conclusions Glucocorticoid inhibit the longitudinal bone growth,to the osteoblasts (OC,BAKP and PINP) of growing rats is not obvious.
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Objective To study the preventive and therapeutic effect of dexamethasone on communicating hydrocephalus following subarachnoid hemorrhage (SAH), and explore the mechanism of communicating hydrocephalus following SAH. Methods SAH rat model was constructed by injecting auto blood through foramen magnum into subarachnoid space. There were two groups ( one control group and one experimental group) in this study with 16 rats each group. Sufficient doses of auto blood were injected into subarachnoid space of the rats of the control group and sufficient dose of auto blood together with sufficient dose of dexamethasone into subarachnoid space of the rats of the experimental group. The changes of rats transforming growth factor bata-1 (TGF-β_1 )in the cerebrospinal fluid (CSF)and thickness of pia mater collagen fibrosis( CF) following SAH were observed after 1 day and 20 days. The levels of TGF-β_1, in the CSF were quantitatively detected by the way of ABC-ELISA. The brain tissues were dyed through Massions technique, and then the average thicknesses of meningina CF were measured by the software of micrograph collection and analysis computer system. Results In later stage after SAH, TGF-β_1, concentrations of the experimental group was lower than control group( P <0. 01). At the same time, the average thicknesses of meningina CF of experimental groups was thinner than control group( P <0. 01). Moreover, the relationship between the level of TGF-β_1 in the CSF and the thickness of the CF of the rat's cerebral pia mate had positive correlation. Conclusions In the later stage after SAH, dexamethasone can inhibit the over expression of TGF-β_1 in rat CSF following SAH and also can inhibit the accrementition of rat collagenous fibers. Therefore, this study provided experimental and theoretical evidence for preventing and treating communicating hydrocephalus following SAH.
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Objective To investigate the immunological mechanism of inhibitory effect of Danshen injection combined with dexamethasone(DXM) on asthmatic airway inflammation.Methods 50 Wistar rats were randomly divided into normal control(NC),asthma,Danshen,DXM and Danshen+DXM group.Cytology study of Bronchoalveolar lavage fluid(BALF) was conducted.Pathology of lung tissue was done through HE.Flow eytometry was used to detect CD4+CD25+ regulatory T Cells(CD4+CD25+ Treg) ratio in peripheral blood mononuclear cells(PBMCs).IL-4 and IL-5 levels in BALF were detected by ELISA.Results Total cells number,percentage of lymphocytes,neutrophils and eosinophils(Eos) in BALF of the three treated groups were lower than that in asthma group(P<0.05,P<0.01),particularly in Danshen+DXM group,which showed significant difference as compared with the other two treated groups(P<0.05).There was severe inflammation in lung tissue of asthma group,moderate inflammation in Danshen group and DXM group,and no inflammation of Danshen+DXM group.CD4+CD25+ Treg/CD4+ T ratio in the three treated groups were higher than that in asthma group,and the levels of IL-4 and IL-5 were lower than those in asthma group(P<0.05).In Dansben+DXM group,it showed significant difference on the change of CD4+CD25+ Treg,IL-4 and IL-5 as compared with other treated groups(P<0.05).Conclision Danshen injection combined with DXM could suppress airway inflammation in asthmatic rats,which may be through increasing the expression of CD4+CD25+ Treg,decreasing the levels of IL-4 and IL-5 and resuming the balance of Th1/Th2.
ABSTRACT
Objective To investigate dexamethasone on airway inflammation and CD4+ CD25 + regulatory T cells (CD4+ CD25 +Tr) of asthmatic rats,and elucidate the possible mechanism of dexamethasone in treatment of asthma.Methods 30 Wistar rats were randomly divided into control group,asthma group and dexamethasone-treated group.Bronchoalveolar lavage fluid (BALF) was collected,and cytology study was conducted.The lung tissue was obtained and pathologic analysis was done through HE stain.Flow eytometry was used to detect the CD4+ CD25 +Tr ratio in PBMCs.Results Total cells number,the percentage of lymphocytes,neutrophils and eosinophils (Eos)in BALF of dexamethasone-treated group were lower than that of asthma group (P＜0.05,P＜0.01).Compared with the asthma group,less infiltration of inflammatory cells in lung tissues was observed in the dexamethasone-treated group.CD4+ CD25 + Tr of asthma group was lower than that of control and dexamethasone-treated group (P＜0.05).Conclusion Dexamethasone could suppress airway inflammation of asthmatic rats,which probably be due to increasing the number of CD4+ CD25 + Tr.
ABSTRACT
This experiment takes the apute experimental serum sickness (AESS) in rabbits as an animal model to investigate the mechanism of the regulatory effects of Dexamethasone (DX) . The results show. DX doesn't inhibit the production of anti-BSA antibody but induces the tendency to increase the level of antibody in rabbits with DX administration on early days (EL, EH); immune complexes(BSA - anti-BSA IGg, CIC) in the rabbits of EL and EH are significantly increased comparing with the later days (LE, LH) and positive controls. Non-specific esterase stainings and the pathological changes of kidney sections are markedly lessened though the amount of anti - BSA antibody, circulating immune complex (CIC) and superoxides dismutase activity in the rabbits of LE and L H are the same as the positive control. The outcome of protecting kidneys of AESS rabbits from injury by DX administration . probadly results from the effects of that DX inhibits infiltration of monocytes into the glomeruli. The role of oxygen radicals in AESS needs further investigation.