ABSTRACT
Objective To investigate the change and possible role of bilirubin between with and without diabetes mellitus in the population of acute ischemic stroke. Methods ⑴A total of 610 hospitali-zation patients at the Department of Neurology in Affiliated Drum Tower Hospital of Nanjing University Medi-cal School from October 2008 to September 2012, who were diagnosed as acute ischemic stroke was divided into two groups based on diabetes mellitus or not, and 138 hospitalization patients with vertigo or dizzy diag-nosed as posterior ischemic while had no acute ischemic stroke were set as a control, divided into two groups based on the same standard too. Also, serum direct bilirubin and total bilirubin and new infarction or not were recorded. ⑵The levels of direct bilirubin and total bilirubin were compared between groups of diabetes and without diabetes, whatever in the population of acute ischemic stroke or not. Results ⑴The levels of serum total bilirubin and direct bilirubin were decreased obviously in the group of diabetes mellitus compared to the group of non-diabetes mellitus in the population of acute ischemic stroke [(17. 085 ± 0. 595)μmol/L vs (18. 920 ± 0. 487)μmol/L, P=0. 024;(4. 362 ± 0. 147)μmol/L vs (4. 876 ± 0. 135)μmol/L, P =0. 019 ] . ⑵There were no obvious difference of serum total bilirubin and direct bilirubin between the groups of diabetes mellitus and non-diabetes mellitus in the population of non-ischemic stroke ( P>0. 05 ) . Con-clusions Induction of bilirubin by acute ischemic stroke can be inhibited by diabetes mellitus, which may be one of the mechanisms of deteriorative brain damage by diabetes mellitus after acute ischemic stroke.
ABSTRACT
Objective To investigate the changes of serum ferritin and homocysteine of diabetic patients,to explore its correlation with diabetic gastroparesis.Methods Pure 50 patients with diabetes,50 patients with diabetic gastroparesis,and 50 cases of normal control group were chosen.The serum ferritin levels were measured with chemiluminescence method,the serum homocysteine levels were measured with enzyme linked immunosorbent assay (ELISA) method.The changes of serum ferritin and serum homocysteine in each groups were observed.Its relationship with diabetic gastroparesis was studied.Results For all diabetic patients,the serum ferritin and homocysteine levels were significantly higher than those in normal control group (P ＜ 0.01).Patients with diabetic gastroparesis was higher than those of pure diabetes (P ＜ 0.01).Serum ferritin levels was positively correlated with glycosylated hemoglobin and homocysteine (r =0.62,0.78,P ＜ 0.01).Multi-factor analysis showed that serum ferritin,and homocysteine level was positively associated with diabetic gastroparesis (P ＜ 0.05).Conclusions The increases of serum ferritin and homocysteine levels were closely related to the occurrence and development of diabetic gastroparesis.They can be used as one of parameters of diabetic gastroparesis,and provide theoretical basis for clinical intervention therapy.
ABSTRACT
Objective To investigate pathological changes of lens epithelium cells (LECs) and expressions of matrix metalloproteinase-3 (MMP-3),alpha-smooth muscle actin (α-SMA),and fibronectin (Fn) in lens epithelial cells of experimental diabetic cataract rats,and to evaluate the roles of MMP-3,α-SMA,and Fn in the pathogenesis of diabetic cataract.Methods A total of 105 healthy Sprague-Dawley (SD) rats without lens diseases was randomly divided into normal control (n =45) and diabetic model (n =60) groups.Diabetic model rats were subjected to intraperitoneal injection of streptozotocin (STZ) (60 mg/kg),and those in the normal control group received injection with 0.1 mmol/L citric acid buffer solution of the same volume.The diabetic models were affirmed upon a fasting blood ≥ 16.65 mmol/L at the 3rd days after the injection.Once a week,the changes of blood glucose and body weight were monitored and the progression of cataract formation in both lenses of all rats was recorded with slit lamp observation.At end of 4 weeks,8 weeks,and 12 weeks after STZ injection,lenses were isolated and embedded in paraffin.The LECs histopathology was examined with HE staining.Immunohistochemical method was used to detect expressions of MMP-3,α-SMA,and Fn in normal and diabetic LECs.The related comparisons and statistic analysis were carried out.Results The lenses of control group were always completely transparent throughout the period of experiment with 31.48％,77.78％,and 100％ of lenses in diabetic model group swelling at 4th,8th,and 12th week,respectively.Under the light microscopic level,it has been showed that lens epithelium cells,which was occurred aggregate plaque and arranged in many layers,presented some morphologic characteristics of fibroblasts by HE staining.In control group LECs regularly,MMP-3,α-SMA,and Fn did not express equally;MMP-3,α-SMA,and Fn expressions increased obviously highly,difference had statistical significance compared to diabetic cataract group LECs (P ＜ 0.01).With the development of course of disease,the differences in expression of MMP-3,α-SMA,and Fn were significant between the diabetic cataract LECs and the control group (P ＜ 0.01).During the progression of diabetic cataract,the expression of Fn was positively correlated with that of α-SMA and MMP-3 (r =0.994,P ＜ 0.01;r =0.993,P ＜ 0.01).Conclusions The diabetic cataract liyingbody animal model was established successfully,which has laid down necessary basis to expound the morbidity mechanism of diabetic cataract.In the lens of diabetic cataract group,the expressions of α-SMA,MMP-3,and Fn were significantly increased.They participated in the occurrence and development of diabetic cataract.The expression of Fn was positively correlated with α-SMA and MMP-3.The lens epithelium cells which took on elongated aspect like fibroblast cells appeared epithelial-mesenchymal transformation (EMT),which mediated the occurrence and development of LECs fibrosis.