Expression of Vascular Endothelial Growth Factor in Diabetic Frozen shoulder / 대한정형외과연구학회지

PURPOSE: To investigate the expression of Vascular Endothelial Growth Factor (VEGF) in diabetic frozen shoulders. MATERIALS AND METHODS: We preformed arthroscopic adhesiolysis on 9 diabetic frozen shoulder patients, and observed the arthroscopic findings. Also, we examined the potential role of VEGF by using samples of synovial tissues from 5 patients, and 2 normal synovial tissues. Immunohistochemical staining and Western blotting were performed using polyclonal antibodies against VEGF. RESULTS: There was hyperemic synovitis in the 9 diabetic frozen shoulder patients. In the 5 patients' tissue samples, there was strong immunostaining and expression to VEGF, but there was little staining and expression in the control group. CONCLUSION: We postulate that VEGF is synthesized and secreted in the synovium of diabetic frozen shoulders and that secreted VEGF binds specific receptors on the endothelial cells of nearby small blood vessels, and leads to the subsequent development of frozen shoulders in diabetic patients.

Role of Vascular Endothelial Growth Factor in Diabetic Frozen Shoulder / 대한정형외과학회잡지

PURPOSE: To investigate the role of VEGF (vascular endothelial growth factor) and macrophage cells in the pathogenesis of diabetic frozen shoulders. MATERIALS AND METHODS: Five diabetic frozen shoulders which were resistant to conservative treatment were involved in this study. Synovial tissue was obtained during arthroscopic adhesiolysis. The synovial tissue of the shoulders from cadava dominators who had no history of shoulder joint disease was used for the control study. Immunohistochemical staining was performed with polyclonal antibodies against VEGF and CD68 macrophage/synovial antigen. RESULTS: Injection and hypertrophy of the synovium and thickening of the capsule were noted in all diabetic frozen shoulders. Strong immunostaining to VEGF and CD68 was observed in all diabetic frozen shoulders, whereas the control specimens showed little staining. CONCLUSION: From these results it would be postulated that the VEGF, synthesized and secreted by macrophage/synovial cells of the diabetic frozen shoulders stimulate regional angiogenesis which leads to synovitis and fibrosis could be one of the leading factors in the pathogenesis of frozen shoulders in diabetic patients.