ABSTRACT
Se realizó un estudio descriptivo transversal de 1 819 pacientes con fiebre y serología reactiva, ingresados en el Hospital Infantil Dr Antonio María Béguez César de Santiago de Cuba por diagnóstico presuntivo de dengue, desde enero de 2015 hasta diciembre de 2016, de quienes se extrajeron muestras de suero en el Departamento de Microbiología de la mencionada institución, las cuales fueron enviadas al laboratorio del Instituto de Medicina Tropical Dr Pedro Kourí de La Habana para su procesamiento y posible confirmación. En la casuística se obtuvo una positividad de 54,3 por ciento, así como predominio de los niños mayores de 5 años con 1 451, de los procedentes del municipio de Santiago de Cuba con 1 651, de la fiebre en 847 y de la ocurrencia de la infección durante el trimestre octubre-noviembre en 864.
A descriptive cross-sectional study of 1 819 patients with fever and reactive serology, admitted in Dr Antonio María Béguez Caesar Pediatric Hospital in Santiago de Cuba was carried out through presumptive diagnosis of dengue from January, 2015 to December, 2016, from whom samples of serum were obtained in the Microbiology Department of the above-mentioned institution, which were sent to the laboratory of Dr Pedro Kourí Tropical Medicine Institute in Havana for their processing and possible confirmation. A 54,3 percent of positive cases was obtained in the case material, as well as the prevalence of children older than 5 years with 1 451, those coming from Santiago de Cuba municipality were 1 651, those with fever were 847 and those with occurrence of the infection during the trimester October-November were 864.
Subject(s)
Infant , Child, Preschool , Child , Adolescent , Physician-Patient Relations , Clinical Diagnosis , Dengue/diagnosis , Symptom Assessment/methods , Serology/methods , Epidemiology, Descriptive , Cross-Sectional StudiesABSTRACT
Background: Dengue fever is currently the most important arthropod borne viral disease. Since occurrence of dengue infections has been an epidemic in many parts of India and complications like DHF and DSS are increasing, while at the same time the diagnosis is challenging, particularly the laboratory diagnosis is confusing, this study was conducted to evaluate the different laboratory test methods and to compare their respective efficacy, timing, advantages and disadvantages.Methods: This study was done in the Department of Microbiology in collaboration with the Department of Medicine and Pediatrics in two tertiary care medical colleges and hospitals in eastern India. Blood samples from 319 patients with clinical features suggestive of Dengue fever were included in this study. Laboratory investigations were done which included immunological assays that were performed using commercially available kits - SD dengue duo NS1Ag + Ab combo rapid test, NS1 Ag capture ELISA, IgM capture ELISA, IgG capture ELISA test for dengue and other routine tests -full blood cell count, coagulation tests, routine biochemical and lipid profile were also done. Ethical considerations were taken care of and statistical evaluations were done.Results: An increased detection of IgM antibody (46.15%) was seen in the early febrile period (1-5 days) as compared to the mid-febrile period (6-10 days), and late febrile period (6-10 days) when it is 6.89%. IgG antibody is much less in early febrile period (4.16%). Compared to mid-febrile period (24.13%), and late febrile period (62.5%). IgM antibodies were detected in 44.5% of the samples, IgG antibodies were detected in 43.5% of the samples, Rapid test was positive in 36.9% and NS1AG ELISA was detected in 43.5% of the samples in the study.Conclusions: It can be inferred from our study that for detection of dengue in the early febrile period (1-5 days), estimation of dengue-specific serum IgM is the most sensitive antibody detection method.