ABSTRACT
Objective: To develop a new method based on dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) for the rapid extraction, cleansing, and determination of iridoid glycosides (loganic acid, morroniside, loganin, sweroside, secoxyloganin, and secologain dimethyl acetal) in Lonicerae Japonicae Flos (LJF) and the related traditional Chinese patent medicine (TCPM) Lianhua Qingwen Capsule (LQC). Methods: Ultrasonic extraction was used for the extraction, different purification material was investigated for the rapid purification, and HPLC method was used for the determination of the content of iridoid glycosides. Results: The purification effect of LJF extract was much better by using diatomite as the purification material; The purification of LQC extract was much better by using diatomite-active carbon 80∶20. This method was simple and rapid, and has high efficiency. It could be used for the extraction, cleaning, and determination of iridoid glycosides in LJF and related prescription combined with HPLC. Conclusion: This study expands the application of DSPE in the extraction and purification of complex matrix traditional Chinese material medicine (TCMM) and TCPM, and supplies the new method and data support for the extraction and purification of bioactive compounds from complex matrix TCMM and TCPM.
ABSTRACT
Objective To prepare a novel bioactive and degradable scaffold with mineralized collagenpolyose based composite by biomimetic synthesis for bone tissue engineering and explore the compatibility of osteoblast culturing on the scaffold. Methods Two kinds of polyelectrolyte were assembled on the surface of diatomite particles in order to adsorbe on nano-zirconia through opposite charges. Zeta potential,particle size and size distribution were compared before and after the modification of diatomite; IR was used to analysis molecular structure of functional group changes on the surface of diatomite particles, nano-composite powder morphology was observed by SEM. Results Two kinds of the polyelectrolyte were successfully assembled on the surface of diatomite powders. Particle size and size distribution were significantly reduced, d (0.5) reduce from 16.421 μm to 0.420 μm. SEM showed the dispersion of the modified diatomite was improved and had a good adsorption with nano-zirconia. Conclusion Layer-by-layer technique could enhance the dispersion of diatomite-based dental ceramic powders as well as a good adsorption of nano-composite ceramic powder.