Simultaneous UV-Spectrophotometric estimation of Diclofenac and Tolperisone Hydrochloride in Tablet Dosage Form.

Diclofenac sodium is a non-steroidal anti-inflammatory drug (NSAID) taken to reduce inflammation and as an analgesic reducing pain in certain conditions. Tolperisone hydrochloride is a piperidine derivative, is a centrally-acting muscle relaxant. Two simple, accurate and economic methods; Q analysis and first order derivative method have been described for the simultaneous spectrophotometric estimation of Diclofenac sodium and Tolperisone hydrochloride in tablet dosage form. Absorption maxima of Diclofenac sodium and Tolperisone hydrochloride in distilled water were found to be 275.0 nm and 260.0 nm respectively. Beer’s law was obeyed in the concentration range 5-50 μg/ml for Diclofenac and 5-60 μg/ml for Tolperisone hydrochloride. In Q analysis method, absorbances were measured at the selected wavelengths, 237.0 nm (isoabsorptive point) and 260.0 nm (λmax of Tolperisone). In first order derivative method, zero crossing point of Diclofenac sodium and Tolperisone hydrochloride were selected at 275.0 nm and 260.0 nm respectively. The analysis of binary pharmaceutical formulation was carried by both methods. Results of two methods were validated statistically by recovery studies and were found to be satisfactory.

Method Development and Validation of Second Order Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac Sodium and Thiocolchicoside from its Pharmaceutical Formulation.

The objective of this study was to evaluate the utility of derivative spectrophotometric method for analysis of Diclofenac sodium (DIC) and Thiocolchicoside (THIO) in combination. Derivative spectrophotometry has allowed specific determination of Diclofenac sodium at 249 nm with negligible contribution by Thiocolchicoside. Similarly, Thiocolchicoside was determined at 246nm with negligible interference by Diclofenac sodium. The described method obeyed Beer’s law in the range of and 4-36μg/ml for DIC 2-10μg/ml for THIO. Validation parameters such as linearity, accuracy, precision, specificity, and LOD and LOQ values were performed. The percentage recovery was 99.52% for DIC and 99.32% for THIO. Intra and Interday precision %RSD values were <2. Thus from the results obtained it can be concluded that proposed method is simple, rapid and specific.