ABSTRACT
Objective: To identify the differential metabolic small molecule substances in Cervi Cornu Pantotrichum (CCP) of different segments based on UPLC-QTOF/MS metabonomics technology, and analyze the metabolic pathways involved by differential metabolites combined with histological observation. Methods: The standard trident antler of sika deer (Cervus nippon) were treated by freeze-drying. The tissue morphology was observed by section. The tissue morphology was divided into three sections from top to bottom, VAU, VAM, and VAB. The differential metabolites were analyzed by UPLC-QTOF/MS technology, principal component analysis, and least squares discriminant analysis. Results: A total of 124 kinds of metabolic small molecular substances were identified in CCP. Based on the FC value of differential metabolites higher than 2.2, 16 substances with different relative contents in different parts of CCP were further screened, and the relative content of each substance in different parts were compared. Conclusion: This experiment is based on non-targeted metabonomics to analyze the composition of metabolic small molecules in freeze-dried CCP. By comparing the adjacent zones, it is found that there are some differences in endogenous metabolites among the zones, which provides data support for revealing their functions.
ABSTRACT
This study was aimed to explore differential effects of various sections of the velvet antler on promoting cell proliferation in vitro. The NRK-49F cell line from rat kidney fibroblasts was used as the cell model. The cell proliferation rates of the water extracts from the upper, middle and lower section of fresh velvet antler were measured by the MTT method. BCA method was used in the detection of protein concentration. The SDS-PAGE method was used in the analysis of difference composition of the sample protein. The results showed that soluble protein content of the upper, middle and lower section were 17.89, 16.04 and 6.89 mg·mL-1, respectively. From the top to the base, the soluble protein content of velvet antler was decreased. After 24 h treatment, when the protein concentration of the upper and middle section samples of the velvet antler were 800 μg·mL-1 and 600 μg·mL-1, the cell proliferation promoting rates reached the maximum, which were 66.76% and 64.36%, respectively. And when the lower section sample of the velvet antler was 1 000 μg·mL-1, the cell proliferation promoting rates reached the maximum, which was 58.87%. After 48 h treatment, when the upper and middle section samples of the velvet antler were 800μg·mL-1, the cell proliferation promoting rates reached the maximum, which were 219.56% and 215.86%, respectively. And when the lower section sample of the velvet antler was 1 000 μg·mL-1, the cell proliferation promoting rates reached the maximum, which was 169.20%. The velvet antler on the proliferation of cells was much better than the 10% fetal bovine serum. The figure of SDS-PAGE showed the slight difference in the protein composition of three part of the velvet antler. It was concluded that all samples had promoting effects on cell proliferation with concentration-depen-dent, and the main protein in different part of the velvet antler had minor differences.