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Objective@#To compare the disinfection effect of three-hole polypropylene infusion bottle stopper by central sterilization method and groove central sterilization method, and to explore the best method of three-hole polypropylene infusion bottle cap disinfection.@*Methods@#A total of 1 088 bottles of three-pore polypropylene infusion bottles from 10 hospitals in Panzhihua City and the surrounding areas of Chengdu were selected for disinfection, and the effects were compared by fluorescent powder tracing method and bacterial culture method respectively. The two methods were divided into two groups: bottle stopper Central Sterilization group and groove Central Sterilization group. Each nurse sterilized two groups of liquid.@*Results@#The total area of fluorescent powder residues after disinfection of 272 bottles of infusion bottles with different specifications M(P25, P75) was 0.26 (0, 2.51). The total area of fluorescent powder residues M(P25, P75) was 15 (8, 24). There were significant differences in the residual area of fluorescent powder between the two groups after liquid bottle disinfection (Z=-17.31, P<0.01). Thirty-four positive specimens of bacteria culture, 68 bacterial colonies and 158 bottles of infusion bottles were disinfected by central sterilization of 160 bottles. There were 15 positive samples and 30 colonies of bacteria cultured in the bottle disinfected by groove central disinfection method (χ2=2.773, P<0.05).@*Conclusion@#The groove center disinfection method is the best disinfection method for the bottle stopper of three-hole polypropylene infusion bottle.
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Objective To investigate the cleanliness status of personal digital assistant(PDA)surfaces, and observe the effect of disposable antiseptic wipes on the cleaning and disinfection of PDA surface.Methods From January to March 2016, 83 daily used handheld PDA in 50 clinical wards of a hospital were as control group, 83 PDA disinfected by disposable antiseptic wipes(CaviWipes)and used in the same environment of the ward at the same time were selected as the intervention group, cleanliness status of PDA surfaces of two groups were detected by adenosine triphosphate(ATP)bioluminescence assay.Results Taken a cut-off value≤100 RLU/100 cm2 as qualified standard of cleanliness, none of PDA in control group was qualified with ATP bioluminescence reading ranged from 189 to 3 379 RLU;after disinfection with CaviWipes, the qualified rate of intervention group was 90.4％(n=75), ATP detection value of PDA surface in all departments were significantly lower than control group(median:[18-28] RLU vs[290-339]RLU, all P＜0.05).Conclusion Microbial contamination on PDA used in hospital wards is common, antiseptic wipes containing quaternary ammonium salt can be used for cleaning and disinfecting surface of PDA.
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Objective To compare different disinfection methods for skin and bottle caps on the detection rate of coagulase negative staphylococcus(CNS)from blood culture,and provide basis for improving collection process and accuracy of blood culture.Methods Blood specimens from the neonatal department of a hospital between June 2012 and August 2014 were collected,blood specimens cultured between June 2012 and June 2013 were as control group (routine disinfection during blood collection process),and blood specimens cultured between August 2013 and Au-gust 2014 were as intervention group (improved disinfection method for skin and bottle caps),detection rates of CNS between two groups were compared.Results In control group,41 isolates of pathogens were isolated from 543 blood culture specimens,positive rate was 7.55%;in intervention group,21 isolates of pathogens were isolated from 853 blood culture specimens,positive rate was 2.46%.The detection rate of CNS in control group was 4.97%(n=27),12 (2.21 %)of which were methicillin-resistant coagulase-negative staphylococcus (MRCNS);the detec-tion rate of CNS in intervention group was 0.82%(n=7),6 (0.70%)of which were MRCNS.Difference in detec-tion rates of CNS and MRCNS between two groups were both statistically significant(χ2 =24.07,5.92,both P <0.05).Conclusion Improving disinfection methods for skin and caps of blood culture bottles during blood collection can decrease the detection rate of CNS.
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Objective To investigate the sanitation status of dental model in medical institutions in Changning District of Shanghai, and to provide evidence for strengthening the disinfection management. Methods 40 medical institutions in Changning District of Shanghai were investigated by field sampling and detection. Results 92.5% of the investigated medical institutions disinfected dental models.In 168 dental model samples, the qualification rate of sanitary quality was 88.1%, while exceeded number of bacteria was the major reason for the unqualified.Among all dental model disinfection methods including ultraviolet irradiation, disinfectant soaking, disinfectant spraying and ozone, ultraviolet irradiation was the most widely used and disinfectant soaking was with the best effect.The storage sites of disinfected dental models including filling room, pollution area of sterilization room and other locations.Dental models stored in the filling room were with the highest qualification rate. Conclusion The current dental model sanitation status in medical institutions in Changning District of Shanghai is relatively unsatisfactory;supervision and inspection administration need to be strengthened by health and family planning administrative departments.
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Objective To carry out bacteriological analysis between re-used and disposable oxygen humidification devices. Methods Reused oxygen equipment and disposable oxygen moisture bottles from 6 randomly selected elderly wards were collected, patients who used oxygen will be randomized into 3 groups, group A used cold water as the oxygen humidification liquid, group B used distilled water as humidification liquid, group C used AQUAPAK disposable oxygen humidification devices, sample taking in group A and group B happened on every Monday and Thursday before the replacement of sample moisture bottles, sample was taken in group C after the use of disposable oxygen humidification devices, according to "hospital disinfection health standards" (GB5982-1995)sampling method of disinfection liquid sample was taken and sent to test in the disinfectant laboratory. Results Of 61 samples in group A bacteria cartier rate was 81.97%, bacteria carrier rate of 71 samples in group B was 47.89%, of 52 samples in group C the carrier rate was 0. The comparing of bacterial contamination rate of three kinds of oxygen moisture bottles after using was statistically significant. Take group A, B for comparison, the average severity level of bacteria in group A is higher than group B. In group A, the level of the Gram-negative bacteria as well as Gram-positive bacteria colonization was higher than group B. Conclusions Whether use of cold water or distilled water, oxygen humidification liquids have different degrees of pollution. Currently there is no general criteria and norms in the context of sterilization of oxygen humidification, for patients on long-term oxygen using, to choose disposable oxygen humidification device to reduce the incidence of hospital infections is recommended.
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OBJECTIVE To explore prevention and control measures and disinfection methods of intestinal virus hospital infection.METHODS Based on the prevention and control of hand-foot-and-mouth disease(2009),the measures on the administration of hospital infection and disinfection techniqul were taken,under the guidance of the center for disease control.RESULTS The effective prevention and control in intestinal virus stopped the spread and cross-infection.CONCLUSIONS Countermeasures of intestinal virus diseases caused by toxic encephalitis are effective following HFMD hospital infection prevention and control measures.
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OBJECTIVE To compare the effect with the three methods to disinfect the ventilator circuits.(METHODS) To disinfect the ventilator circuits,three methods were used:A,soaking with chlorine compoud(disinfectant);B:soaking with 0.5% peracetic acid;C,sterilizing with ethylene oxide.Samples were taken to(bacteria) culture before and after disifection in every three methods.Biological supervision and the amount of colony were also taken at the same time.RESULTS The results of bacteria culture pre-disinfection were all positive.The amount of bacteria of ventilator circuits was between 70 000-120 000CFU/cm~2.The eligible rate of biological(supervision) in method C was 100% and its positive rate of bacteria culture was 0%.The eligible rate of biological supervision in method A was 86.25% and its positive rate of bacteria culture was 16.25%.The eligible rate of(biological) supervision in method B was 90% and its positive rate of bacteria culture was 13.78%.It was significant when method C was compared with methods A and B and the value P was less than 0.01.CONCLUSIONS It is(serious) about the pollution in ventilator circuits.The soaking methods are apt to be effected by the factors of(environment) and manual operation.Therefore it could be(ineligible) and being polluted after disinfection.The method of sterilization is the best for disinfection.