Use of the activPAL® triaxial accelerometer to estimate total energy expenditure in low-income women: differences between body mass index classifications

ABSTRACT Objective: This study aimed to assess the agreement between the total energy expenditure (TEE) estimated by the activPAL® triaxial accelerometers (ACC) and the TEE measured by the doubly labeled water method (DLW), as well as to assess if these values differ between the classifications of body mass index (BMI). Materials and methods: This is a cross-sectional study. Low-income adult women (19-45y) with BMI ≥ 18.5 kg/m2 were included. Accelerometry data (activPAL®) were collected over 7 consecutive days, which were used to calculate TEE-ACC and compared with DLW data. The Bland-Altman method, concordance correlation coefficient and root mean square error were used to assess agreement between methods. Results: The sample consisted of 55 women with a mean age of 31 ± 5 years. The agreement between TEE-ACC and TEE-DLW showed a bias of -142.5 kcal (-7.1%). Among the BMI classifications, participants with normal weight show a bias of -417.1 kcal (-21.0%), participants with overweight, -87.5 kcal (-3.9%) and participants with obesity, 97.5 kcal (4.3%). Furthermore, the bias between the methods showed a significant and positive correlation with the body weight (r = 0.49; p < 0.01). Conclusion: The TEE-ACC estimates from activPAL® were reasonably accurate when compared to the TEE-DLW, especially in women with overweight and obesity, being much less accurate in individuals with normal weight.

Measurement of the Thermic Effect of Food in a Chinese Mixed Diet in Young People / 生物医学与环境科学（英文）

OBJECTIVE@#To determine the thermic effect of food (TEF) in a Chinese mixed diet in young people.@*METHODS@#During the study, the participants were weighed and examined for body composition every morning. The total energy expenditure (TEE) of the participants was measured by the doubly labeled water method for 7 days, and during this period, basal energy expenditure was measured by indirect calorimetry and physical activity energy expenditure was measured by an accelerometer. The value obtained by subtracting basal energy expenditure and physical activity energy expenditure from TEE was used to calculate TEF.@*RESULTS@#Twenty healthy young students (18-30 years; 10 male) participated in the study. The energy intake of the participants was not significantly different from the Chinese Dietary Reference Intake of energy ( P > 0.05). The percentage of energy from protein, fat and carbohydrate were all in the normal range. The intakes of fruits, milk and dietary fiber of the participants were significantly lower than those in the Chinese Dietary Guidelines ( P < 0.05). There was no significant difference in the body weight of the participants during the experiment ( P > 0.05). When adjusted for body weight, there was no significant difference in either TEE or basal energy expenditure between the male and female participants ( P > 0.05). In addition, there was no significant difference in physical activity energy expenditure and TEF between the male and female participants ( P > 0.05). The percentage of TEF in TEE was 8.73%.@*CONCLUSION@#The percentage of TEF in TEE in a Chinese mixed diet in young people was significantly lower than 10% ( P < 0.001). A value of 10% is usually considered to be the TEF in mixed diets as a percentage of TEE.

Accuracy of 24-hour Diet Recalls for Estimating Energy Intake in Elderly Men using the Doubly Labeled Water Method / 대한지역사회영양학회지

OBJECTIVES: This study assessed the accuracy of the 24-hour diet recall method for estimating the energy intake of elderly men using the doubly labeled water as a reference method. METHODS: Seventeen subjects (mean age 72.5 ± 3.9 years), who maintained the same body weight during the two weeks study period, were included in this study. Three 24-hour diet recalls (two weekdays and one weekend) were obtained over a 14 day period to estimate the mean energy intake. The total energy expenditure was measured over the same 14 days using the doubly labeled water method. The total energy intake and total energy expenditure were compared by paired t-test. RESULTS: The total energy intake from the 24-hour diet recalls method was 2536.7 ± 350.6 kcal/day, and the total energy expenditure from the doubly labeled water method was 2659.8 ± 306.8 kcal/day. The total energy intake was slightly under-reported by −123.2 ± 260.8 kcal/day (−4.4%). On the other hand, no significant difference was observed between the total energy intake and total energy expenditure of the subjects (p=0.069). The percentage of accurate predictions was 64.7%. The correlation between the total energy intake and total energy expenditure was statistically significant (r=0.697, p < 0.005). CONCLUSIONS: The present study supports the use of the 24-hour diet recall method to estimate the mean energy intake in elderly men group. More studies are needed to assess the validity of 24-hour diet recall method in other population groups, including elderly women, adults and children.

Accuracy of 24-hour Diet Recalls for Estimating Energy Intake in Elderly Men using the Doubly Labeled Water Method / 대한지역사회영양학회지

OBJECTIVES: This study assessed the accuracy of the 24-hour diet recall method for estimating the energy intake of elderly men using the doubly labeled water as a reference method. METHODS: Seventeen subjects (mean age 72.5 ± 3.9 years), who maintained the same body weight during the two weeks study period, were included in this study. Three 24-hour diet recalls (two weekdays and one weekend) were obtained over a 14 day period to estimate the mean energy intake. The total energy expenditure was measured over the same 14 days using the doubly labeled water method. The total energy intake and total energy expenditure were compared by paired t-test. RESULTS: The total energy intake from the 24-hour diet recalls method was 2536.7 ± 350.6 kcal/day, and the total energy expenditure from the doubly labeled water method was 2659.8 ± 306.8 kcal/day. The total energy intake was slightly under-reported by −123.2 ± 260.8 kcal/day (−4.4%). On the other hand, no significant difference was observed between the total energy intake and total energy expenditure of the subjects (p=0.069). The percentage of accurate predictions was 64.7%. The correlation between the total energy intake and total energy expenditure was statistically significant (r=0.697, p < 0.005). CONCLUSIONS: The present study supports the use of the 24-hour diet recall method to estimate the mean energy intake in elderly men group. More studies are needed to assess the validity of 24-hour diet recall method in other population groups, including elderly women, adults and children.

Energy metabolism and body composition in athletes / 体力科学

In this review, the energy metabolism and body composition in athletes are discussed on the basis of an energy balance study using a doubly labeled water method. In athletes, the energy expenditure induced by sports activities widely differs according to the type of sports event. In addition, the physical activity of athletes can reach more than twice the basal metabolic rate throughout the sports season. It may be also more than three times depending on the duration of the tournament and/or the training camp. Thus, the event type, and training time and intensity must be considered when estimating the energy requirement of athletes.

Estimating free-living human energy expenditure: Practical aspects of the doubly labeled water method and its applications

The accuracy and noninvasive nature of the doubly labeled water (DLW) method makes it ideal for the study of human energy metabolism in free-living conditions. However, the DLW method is not always practical in many developing and Asian countries because of the high costs of isotopes and equipment for isotope analysis as well as the expertise required for analysis. This review provides information about the theoretical background and practical aspects of the DLW method, including optimal dose, basic protocols of two- and multiple-point approaches, experimental procedures, and isotopic analysis. We also introduce applications of DLW data, such as determining the equations of estimated energy requirement and validation studies of energy intake.

Total energy expenditure of 16 Chinese young men measured by the doubly labeled water method / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men.</p><p><b>METHODS</b>Sixteen healthy young men age 23±1 years with body mass index 22.0±1.4 kg/m2 were recruited. TEE was measured by the DLW method, and basal energy expenditure (BEE) was determined by indirect calorimetry. We also conducted 24-h activity, energy balance and factorial approach to estimate energy requirements of the subjects.</p><p><b>RESULTS</b>TEE of subjects by DLW method was 9.45±0.57 MJ/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 MJ/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 MJ/day (2373±315 kcal/day). The BEE of 6.65±0.28 MJ/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P<0.001) than that measured by indirect calorimetry, 5.99±0.66 MJ/day (1433±158 kcal/day). The TEE derived from the factorial approach was 10.31±0.43 MJ/day (2463±104 kcal/day).</p><p><b>CONCLUSION</b>The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement.</p>

Relations between daily energy expenditure and body fatness, physical fitness in primary school children using doubly labeled water method and accelerometer / 体力科学

The relationships between physical activity and childhood body size, low physical fitness epidemic are still unclear. The purpose of this study was to examine the relationship between energy expenditure estimated by doubly labeled water (DLW) method and body fatness, physical fitness in children, and the relationship between physical activity levels and percent time spent in activities. 30 healthy Japanese children (20 boys and 10 girls) participated in this study. The total energy expenditure (TEE) and % body fat were measured by the DLW method over a 6-day period. The physical activity-related energy expenditure (PAEE) was calculated as (TEE × 0.90) – basal metabolic rate (BMR). The physical activity level (PAL) was also calculated as TEE/BMR. The physical fitness tests (8 items) were applied to evaluate fitness, and scores of each test were calculated as overall physical fitness score. The TEE was 2009.8 ± 272.6 kcal/day, the PAEE was 558.4 ± 206.1 kcal/day and the PAL was 1.61 ± 0.18. TEE per weight and PAEE per weight (PAEE/wt) was significantly negatively correlated with % body fat (r = - 0.626; r = - 0.400, respectively). These results suggest that increasing energy expenditure is important for achieving adequate body size. The PAEE/wt was most strongly correlated with physical fitness score (r = 0.680). The PAL was associated with percent time spent of inactivity ( r = -0.506), light-moderate activity ( r = 0.450) and vigorous activity ( r = 0.545). It was suggested that physically active lifestyle would be necessary for childhood health.

Mitochondrial Dysfunction and Insulin Resistance: The Contribution of Dioxin-Like Substances

Persistent organic pollutants (POPs) are known to cause mitochondrial dysfunction and this in turn is linked to insulin resistance, a key biochemical abnormality underlying the metabolic syndrome. To establish the cause and effect relationship between exposure to POPs and the development of the metabolic syndrome, Koch's postulates were considered. Problems arising from this approach were discussed and possible solutions were suggested. In particular, the difficulty of establishing a cause and effect relationship due to the vagueness of the metabolic syndrome as a disease entity was discussed. Recently a bioassay, aryl-hydrocarbon receptor (AhR) trans-activation activity using a cell line expressing AhR-luciferase, showed that its activity is linearly related with the parameters of the metabolic syndrome in a population. This finding suggests the possible role of bioassays in the analysis of multiple pollutants of similar kinds in the pathogenesis of several closely related diseases, such as type 2 diabetes and the metabolic syndrome. Understanding the effects of POPs on mitochondrial function will be very useful in understanding the integration of various factors involved in this process, such as genes, fetal malnutrition and environmental toxins and their protectors, as mitochondria act as a unit according to the metabolic scaling law.

ASSESSING DAILY PHYSICAL ACTIVITY IN ELEMENTARY SCHOOL STUDENTS USED BY ACCELEROMETER / 体力科学

The purpose of present study was to examine validity of accelerometer (Lifecorder ; LC) and to assess the free-living physical activity (PA) in Japanese elementary school students used by LC method. Firstly, to examine validity of LC, twelve children, aged 11.2±1.0 years, were measured total energy expenditure (TEE) by doubly labeled water method and resting energy expenditure (REE) by the expired gas analysis. Physical activity energy expenditure (PAEE) is calculated as 0.9 TEE minus REE. At the same time, PA was evaluated by both daily walk count (WC) and vigorous activity time (LC7-9) using LC method. PAEE per 1 kg body weight significantly correlated with WC (r=0.785, p<0.005) and LC7-9 (r=0.828, p<0.001). Secondly, 140 boys and 167 girls, aged 8-11 years were measured PA by LC method. WC and LC7-9 each were 13000-18000 counts and 16-32 minutes in week-day, and 8000-12500 counts and 8-18 minutes in week-end. PA analyzed by MANOVA were significantly higher in boys than in girls, and lower in week-end than in week-day, and decreased in proportion as grade. Present study suggests that LC method is a good measure of PA in free-living condition for elementary school students.

VALIDITY OF METHODS TO MEASURE TOTAL ENERGY EXPENDITURE OF BASEBALL PLAYERS IN JAPANESE HIGH SCHOOL / 体力科学

The purpose of this study was to assess the validity of activity record (AR), accelerometer (AC) and heart rate (HR) methods in comparison with the doubly-labeled water (DLW) method as the criterion measure of total energy expenditure (TEE) in high school students undergoing vigorous activities. Twelve baseball players in Japanese high school (16.5±0.5 yrs) were monitored for seven consecutive days. TEE values obtained over a 3-day-period by AR, AC and HR were simultaneously validated against TEE measured by DLW over a 7-day-period. TEE value measured by DLW was 4922±391 kcal/day. The Physical activity level (PAL=TEE/BMR (basal metabolic rate)) was 2.66±0.14. TEE estimated by AR, AC and HR was significantly different from DLW, with mean differences of -13.9±6.5%, 35.3±3.6% and 31.6±23.9%, respectively. However, TEE estimated by AC and AR significantly correlated with that measured by DLW (r=0.73, r=0.70, p<0.05, respectively). The limits of agreement between DLW and AR, AC and HR were from -1350 kcal/d to -21 kcal/d, -2293 kcal/d to -1192 kcal/d, and 3875 kcal/d to -797 kcal/d respectively. AC was superior to the other methods. However, the limits of agreement in AR were similar to AC. These data suggest that on accuracy and precision, AR would be superior to the other two for predicting TEE in high school students undergoing vigorous activities. However, AC has the advantages being able to examine patterns of activity over long periods of time without interfering with normal movement and the ability to store data continuously over long periods of time. Therefore, it was suggested that, simultaneously, the AR and AC methods are accurate predictors of TEE and patterns of activity while undergoing sports vigorous activities.

ENERGY EXPENDITURE, BODY COMPOSITION AND MAXIMAL OXYGEN UPTAKE IN MIDDLE-AGED JAPANESE WOMEN WHO HAVE LONG-TERM HABITS OF EXERCISING / 体力科学

The purpose of this study was to examine the effects of long-term habitual exercise on daily total energy expenditure (TEE) and its components, body composition and maximal oxygen uptake (VO₂max) in middle-aged Japanese women. Twenty-eight subjects aged 39 to 58 years were assigned either to a nonhabitual exercise group (Control ; n=12) or a habitual exercise group (Exercise ; n=16). TEE, physical activity level (PAL) and the daily physical activity energy expenditure (PAEE) were assessed by doubly labeled water (DLW) method. The exercise-induced energy expenditure (ExEE) and nonexercise-induced energy expenditure (NExEE) were evaluated based on the activity record investigation conducted simultaneously during the DLW measurement period. The result follows that TEE was significantly (p<0.01) higher in the Exercise group (2520 kcal · day^-1) than in the Control group (1921 kcal · day^-1). There was no significant difference in basal metabolic rate between the groups. PAEE and ExEE were significantly higher in the Exercise group than in the Control group (p<0.01). Habitual exercise induced an increase in TEE without resulting in a compensatory reduction of NExEE. The percentage body fat (fat%) was significantly lower in the Exercise group (25.5%) than in the Control group (30.9%). VO₂max was higher in the Exercise group (1788 ml · min^-1) than in the Control group (1417 ml. min^-1). After correction for body weight, fat% was negatively associated with TEE (p<0.01), PAEE (p<0.01) and ExEE (p=0.05), while VO₂max was positively associated with TEE (p<0.05), PAL (p<0.05), PAEE (p<0.05) and ExEE (p<0.01). These results suggest that habitual exercise is associated with the increase of TEE, the improvement of body composition and VO₂max. The improvement of VO₂max was mainly caused by the increase in ExEE, indicating that the exercise intensity is important to the improvement of cardiorespiratory endurance fitness.

MEASUREMENT OF TOTAL ENERGY EXPENDITURE IN JAPANESE FIREFIGHTERS UNDER NORMAL WORKING CONDITION USING THE DOUBLY LABELED WATER METHOD / 体力科学

This study examined total energy expenditure (TEE) of Japanese firefighters under normal working conditions, and also evaluated several field methods for measuring TEE in comparison with the doubly labeled water (DLW) method. Ten male subjects belonging to Tokyo metropolitan firefighters departments, aged 30.0±2.8 yrs (mean±SD), were monitored for five consecutive days. While TEE was assessed by an accelerometer (AC) and activity records (AR) in additoin to DLW, energy intake (El) was evaluated by dietary record. There were three emergency calls over the assessment period, but no real operation of fire suppression. TEE obtained by the DLW method (4009±611 kcal/d) was significantly higher than that estimated by AC (2531±186 kcal/d) or AR (3704±476kcal/d) ; but significantly correlated with AR (r=0.876, p<0.01) . El (3539±286 kcal/d) showed no significant relationship with DLW. These results suggest that compared with AC, AR would be more feasible for estimating TEE of firefighters, who perform special activities carrying heavy equipment. The mean value of physical activity level (PAL) (2.2±0.3) indicates that firefighters can be categorized as heavy workers like soldiers or policemen.

Comparative study of total energy expenditure in japanese men using doubly labeled water method against activity record, heart rate monitoring, and accelerometer methods / 体力科学

The purpose of this study was to examine total energy expenditure (TEE) in Japanese men under free-living conditions using various field methods, and compare these methods with the doubly labeled water method (DLW) . Ten Japanese men, aged 24.2±1.8 (mean±SD) yrs, were studied for 14 consecutive days. TEE was assessed by DLW, activity record method (AR ; using relative metabolic rate and calculated basal metabolic rate), heart rate monitoring methods (Two-line and FLEX-HR methods), and accelerometer method (AC) . Energy intake (EI) was also evaluated over the same period. Although TEE estimated by AR (2730±139 kcal⋅day<SUP>-1</SUP>), Two-line (2925±433 kcal⋅day<SUP>-1</SUP>), and FLEX-HR (2949±506 kcal⋅day<SUP>-1</SUP>) did not differ significantly from the TEE determined by DLW (2910±524 kcal⋅day<SUP>-1</SUP>), there was no significant relationship between each of these methods and DLW. In addition, El (2963±482 kcal⋅day<SUP>-1</SUP>) and TEE determined by DLW were similar, and there was a significant correlation (r=0.809, P<0.01) . Compared with DLW, AC (2697±541 kcal⋅day<SUP>-1</SUP>) underestimated TEE at the group level ; however, AC was the only method to show a significant correlation with DLW (r=0.871, P<0.001) . Therefore, it seems possible that AC would accurately estimate TEE at the individual level by improving both the instrument and its methodology.