ABSTRACT
Objective To study the effect of brucine on the growth of a hepatocellular carcinoma cell line in vitro. Methods Brucine was added into a liver cancer cell line of SMMC-7721 in vitro, at drug concentration of brucine from 2. 5 μg/ml to 400 μg/ml. The inhibition rate of cell growth was measured by MTT technique after the cells were cultured for 72 hours. The protein and mRNA expression of PCNA,cyclin D1 and FAS were respectively assayed with Western blotting and fluorescent quantitation RT-PCR techniques at 24, 48, 72 h. Results The inhibition rate of liver cancer cell was near 100% when the brucine concentration was at 320 μg/ml. The protein and mRNA expression of FAS were of no significant difference at 24 h vs 48 h ( seperately F = 2. 547,1. 582, all P ＞ 0. 05 ), and significant difference existed at 24 h vs 72 h( seperately F = 1. 036, 1. 137, all P ＜ 0. 05 ). The protein and mRNA expression of PCNA,Cyclin D1 were of no significant difference between various time period( seperately PCNA F = 3.612,2. 174,3.029;Cyclin D1 F=2.361,2.915,1.976,all P＞0.05). Conclusions Brucine inhibits the growth of liver cancer cells, by inducing increased apoptosis of the cells probably through FAS overexpression.
ABSTRACT
ATP-tumor chemosensitivity assay(ATP-TCA)is a tumor chemosensitivity assay in vitro.Beacause of the special merites of A11P-TCA,it is seemed to be the most promising and effective method of tumor chemosensitivity in vitro.Application of ATP-TCA in clinic has got significant effects.ATP-TCA is a good method for individualized chemotherapy.
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Objective To investigate the relationship between the sensitivity of colon carcinoma cells to epidermal growth factor receptor(EGFR) inhibitor,gefitinib,and the downstream proteins of EGFR.Methods The expressions of EGFR proteins of 6 colon carcinoma cell lines(Lovo,HCT116,HT29,LS174T,SW480 and SW620) were determined with immunocytochemistry staining.The inhibitory effects of gefitinib on the growth of colon carcinoma cells were assessed by MTT,and the expression levels of Akt and MAPK as well as their phosphorylated forms(p-Akt and p-MAPK) were assessed by Western blotting.Results EGFR protein expressed in all the Lovo,HCT116,HT29,LS174T and SW480 cells,and the highest expression was found in Lovo cells,but not in SW620 cells.Lovo cells showed the highest,HT29 and SW480 cells showed moderate,sensitivity to gefitinib,while the others showed more or less resistance to gefitinib.No significant difference was found between the growth inhibition and IC50 values among the 6 cell lines despite of being treated with fetal bovine serum or EGF.Akt protein existed in all the cell lines without notable difference.Lovo and SW620 cells showed the least of p-Akt expression(P
ABSTRACT
Objective To investigate the antitumor activity of Gefitinib, a selected epidermal growth factor receptor-tyrosine kinase inhibitor, on human colorectal cancer cell lines in vitro, and to explore the relationship between the inhibitory effect of Gefitinib on cancer cells and the expression of epidermal growth factor receptor (EGFR). Methods The growth inhibitory effects of Gefitinib, which expressed as the half growth inhibition dose IC50, on colorectal cancer cells were assessed by MTT assay. EGFR mRNA expression was detected by reverse transcriptional PCR (RT-PCR). Western blot was used to determine the expression of EGFR protein as well as its phosphorylated forms (p-EGFR). Results Gefitinib inhibited growth of all the six colorectal cancer cell lines in vitro with an IC50 range from 6.5 to 172.7?mol/L. Lovo cell line, with an IC50 value less than 10?mol/L, was the most sensitive one to Gefitinib, HT29 and SW480 were moderate sensitive to 10?mol/L