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Objective::To observe the effect and mechanism of modified Si Junzitang combined with heat-sensitive moxibustion on interleukin-17(IL-17), interleukin-22(IL-22), interleukin-1α(IL-1α) and serum cystatin C(Cys-C )in serum and exhale breath condensate(EBC) of patients with chronic obstructive pulmonary disease at stable stage(COPD, Qi deficiency of lung and spleen). Method::Totally 120 cases of COPD(Qi deficiency of lung and spleen) treated in pulmonary department and thermal moxibustion department of Affiliated Hospital of Jiangxi University of traditional Chinese medicine from January 2019 to June 2019 were included and randomly divided into traditional Chinese medicine group, heat-sensitive moxibustion group and control group. The patients in traditional Chinese medicine group were treated with Si Junzitang, the patients in heat-sensitive Moxibustion group were treated with heat-sensitive moxibustion in addition to traditional Chinese medicine group, and the patients in control group were treated with placebo. All of the 3 groups were treated with oxygen and bronchodilator according to the guidelines. All groups received 3 consecutive courses of treatment, 20 days per course. After 3 courses of treatment, the clinical efficacy of the three groups, the forced expiratory volume in one second (FEV1), the forced expiratory volume in the estimated value in one second (FEV1%), the forced vital capacity (FVC), and IL-17, IL-22, IL-1α in serum and exhale breath condensate (EBC) were measured. Result::There were no statistically significant difference in general clinical data, lung function levels (FEV1, FEV1%, FVC), serum and EBC levels of IL-17, IL-22, IL-1α and Cys-C in the first three groups. The total clinical effective rate of traditional Chinese medicine group was better than the control group (P<0.05), the heat-sensitive moxibustion group was better than the traditional Chinese medicine group (P<0.05) and significantly better than the control group (P<0.01). Compared with the patients before treatment, the level of lung function was improved, while IL-17, IL-22, IL-1α and Cys-C in serum and EBC were reduced(P<0.05). The traditional Chinese medicine group was superior to that in the control group (P<0.05), the heat-sensitive moxibustion group was superior to that in the traditional Chinese medicine group (P<0.05) and significantly superior to that in the control group (P<0.01). Conclusion::Modified Si Junzitang combined with heat-sensitive moxibustion has an anti-inflammatory effect on COPD by stimulating bullishness of human body, improving body immunity, inhibiting inflammatory cytokines, reducing levels of inflammation cytokines IL-17, IL-22, IL-1α, and chronic inflammation markers serum Cys-C and inflammatory reaction, increasing the lung capacity, improving ventilation function and pulmonary function, so as to effectively relieve chest tightness asthma and other symptoms in COPD patients, and improve the clinical efficacy.
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Objective To investigate the clinical significance of biochemical markers in EBC and serum in patients with bronchial asthma and chronic obstructive pulmonary disease overlap syndrome(ACOS). Methods We selected Patients with chronic airway inflammatory diseases in our hospital,These patients underwent clinical trial after the stable phase,including 18 ACOS patients,22 asthma patients,24 COPD patients and 20 healthy non-smokers in the same period.8-isoPG and other inflammatory factors levels in EBC and serum were measured in the selected patients. A comparative analysis was performed. Results The levels of EBC 8-isoPG in the ACOS group were significantly higher than those in the healthy control group,The levels of serum and EBC 8-isoPG in the ACOS group were significantly higher than the asthma group and the COPD group(P < 0.05). The level of 8-isoPG in EBC was not related to age,smoking index,weight,and FEV1value(P>0.05).Conclusions Inflam-matory factors including 8-isoPG,are involved in chronic inflammation in lung tissues of patients with ACOS. 8-isoPG in EBC may have potential value in identifying ACOS from COPD and asthma as biomarkers and deserve further study.
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Diante das ameaças de retrocessos políticos e sociais no país, que incluem o desmonte da TV Brasil e da Empresa Brasil de Comunicação (EBC), esta nota discute a importância da comunicação pública para associedades democráticas. A análise parte de referências históricas, conceituais, e da relação entre Estado e comunicação. Aborda a relação intrínseca entre comunicação pública e o direito à comunicação e sua centralidade nas instituições estatais de caráter público. A partir do modelo de comunicação pública desenvolvido na Europa ocidental, situa a criação da EBC como marco histórico da comunicação pública no país, e apresenta exemplos de sua trajetória recente que expressam sua maior integração com a sociedade.
Given the threats of political and social retrogressions in Brazil, including the policy of dismantling the TV Brasil and the Empresa Brasil de Comunicação (EBC), this note discusses how is important the public communication to democratic societies. Historical and conceptual references as well as the relations between the state and communication are the starting point for the analysis. It deals with the inherentrelation between public communication and the communication rights and its centrality in the public nature of governmental institutions. Based on the model of public communication developed in Western Europe, this note shows that EBC represents a milestone in the Brazilian public communication history and gives examples of its recent trajectory indicating that it was more and more integrated into national society.
Frente a las amenazas de los retrocesos políticos en el país, que incluyen el desmantelamiento de la TV Brasil y de la Empresa Brasil de Comunicação (EBC), esta nota examina la importancia de la comunicación pública para las sociedades democráticas. El análisis parte de referencias históricas, conceptuales y dela relación entre Estado y comunicación. Trata de la relación intrínseca entre comunicación pública y elderecho a la comunicación y su centralidad en las instituciones estatales de carácter público. A partir del modelo de comunicación pública desarrollado en Europa Occidental, establece la creación de la EBC como marco histórico de la comunicación pública en lo país y presenta ejemplo de su trayectoria reciente que expresan su mayor integración con la sociedad.
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Humans , Access to Information , Communications Media , Television , Brazil , DemocracyABSTRACT
Aim To investigate the anti-tumor effects of FS-108 an Hsp90 inhibitor, on oncogene addicted EBC-1 and A375 cells. Methods SRB assay was performed to investigate cell proliferation. Immunoblot was conducted to investigate the specific proteins. FACS was conducted to test cell cycle distribution and apoptosis. Transwell assay was conducted to investigate cell motility. Results FS-108 significantly suppressed cell proliferation of EBC-1 and A375 cancer cells with IC50 at 25. 53 nmol · L-1 and 30. 02 nmol · L-1 re-spectively. FS-108 treatment triggered the degradation of key client proteins such as c-Met and B-Raf and thereby reduced their downstream AKT and ERK signa-ling pathways. The FACS analysis results demonstrated that FS-108 treatment induced G2/M phase arrest and apoptosis significantly. Furthermore, FS-108 inhibited the migration of EBC-1 and A375 cells. Conclusion As a potent Hsp90 inhibitor, FS-108 can inhibit onco-gene addicted cancer cells proliferation through induc-tion of G2/M phase arrest and apoptosis.
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Una de las especies de ácaros que producen considerables pérdidas cualitativas y cuantitativas en el cultivo de rosas bajo invernadero en Ecuador es Tetranychus urticae, donde el control con agroquímicos convencionales no es efectivo; por lo cual se busca identificar cepas de Bacillus spp. aisladas a partir de T. urticae y determinar sus escenarios anatómicos patogénicos para un futuro control con bacterias. La fase de campo se realizó en la florícola Naranjo Roses S.A. en Latacunga en un cultivo de rosas bajo condiciones orgánicas, en donde se recolectaron hojas de cada tercio de seis plantas con presencia de T. urticae Koch. Las muestras fueron trasladadas para ser procesadas en Plantsphere Laboratories (PSL) en Quito. Se identificaron y cuantificaron los diferentes estadios de la plaga y se aislaron algunos individuos para determinar la presencia de cepas patogénicas de Bacillus spp., las cuales fueron aisladas, purificadas e identificadas. Los tratamientos de verificación patogénica fueron las cepas, agua destilada (testigo) y Bacillus thuringiensis biovar acari (testigo positivo). Los eventos patogénicos se evaluaron en hembras adultas de T. urticae mediante citohistoquímica, por medio de la cual se reportó ruptura de paredes externas, precipitación de contenido celular y malformaciones cuticulares. Se determinó que el mayor número de individuos plaga se localizan en el tercio bajo (59,4%) con mayor presencia de huevos (63,3%). Mediante análisis de componentes principales (ACP) de los tratamientos, se determinó las cepas más eficientes como Efectores Biocatalíticos (EBc©)siendo estas la PSL 104, 113, 114 y Bacillus thuringiensis biovar acari.
One of the species of mites that cause considerable qualitative and quantitative losses in roses growing under greenhouses in Ecuador is Tetranychus urticae, where conventional agrochemicals control is not effective; therefore seeks to identify strains of Bacillus spp. Isolated from T. urticae and determine their anatomical pathogenic scenarios for future control with bacteria. The field phase took place in Naranjo Roses S.A. in Latacunga in a growing roses under organic conditions, where they were collected sheets of each third of six plants with presence of T. urticae Koch. The samples were taken to be processed in Plantsphere Laboratories (PSL) in Quito. They were identified and quantified the different stages of the pest and some individuals were isolated for the presence of pathogenic strains of Bacillus spp., which were isolated, purified and identified. Check treatments of pathogenic were strains, distilled water (control) and Bacillus thuringiensis biovar acari (positive control). Pathogenic events were evaluated in adult females of T. urticae by citohistochemistry, where breakout of the outer walls, precipitation of cell contents and cuticular malformations were reported. It was determined that the largest numbers of pest individuals are located in the lower third (59.4%) with greater presence of eggs (63.3%). Using principal component analysis (PCA) of the treatments, the PSL 104, 113, 114 and Bacillus thuringiensis biovar acari was determined as the most efficient Biocatalytic Effectors (EBc©).
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Aims: Predictive and prognostic features have served to allow prognostication for patients with early stage breast cancer. We sought to document our own outcomes for these features to see if our cohort corresponded to published reports. Study Design: Retrospective pilot cohort study. Place and Duration of Study: Department of Medical Oncology, Mid-Western Cancer Centre (MWCC), Mid-Western Regional Hospital (MWRH), Limerick, Ireland, between 1st January 2002 and 31st December 2002. Methodology: A retrospective analysis was designed to include with early stage breast cancer seen at our institution for the aforementioned period, information was derived from the patients’ records and indices were derived from prognostic tools. Information was analyzed using descriptive statistics and χ2 or Fisher’s exact test. Results: Seventy-seven (77) patients were found, with a median age of 52.2 years. Median overall survival of 84 months for the 10-year period of follow-up. The majority presented with moderately differentiated oestrogen receptor positive invasive ductal carcinoma and lymph node involvement (60%). 64% of patients underwent mastectomy as opposed to breast conservation. Adjuvant cytotoxic chemotherapy uptake was 61%, which was comparable to proportion of node positive disease. The predictive and prognostic features including axillary nodal status, tumour size, tumour grade, age at presentation and oestrogen receptor status were all significant indicators for outcome, but particularly within patients under 50 years of age. Conclusions: This report underscores that these predictive and prognostic factors were more significant within for patients under the age of 50 years.
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Background and purpose:The prognosis in patients with early breast cancer(EBC) was poorly defined by clinical and histopathological features.There were more than 200 genes that might be correlated with development,progression,recurrence and prognosis of breast cancer in the published literature.This study evaluated the prognosis of patients with EBC at molecule level according to the protein expressions of 16 selecting genes(HER2,ER,PR,BCL2,Ki-67,BMYB,Cyclin B1,STK15,MMP11,BAG1,NM23,PTEN,P53,P27,VEGF,PCN A).Methods:The immunohistochemical method was used to detect the protein expressions of these genes in 76 patients with EBC and the statistical analysis was performed by COX proportional hazards model.Results:COX proportional hazard model revealed that BCL2,VEGF and STK15 were independent prognostic factors for patients with EBC.A formula of PI was set up according the three genes.The predictive outcomes with PI were compared with the actual follow-up outcomes in 48 patients with EBC.As a result,the predictive accuracy of good and bad prognosis was 86.67% and 91.67%,respectively.Conclusion:A formula of PI derived from protein expressions of the 16 genes correlated with breast cancer was ultimately set up and might be used to predict the outcomes of the patients with EBC.