ABSTRACT
Cytochemical methods were used to evaluate the changes of intracellular calcium, plasma membrane permeability and their relationship during ischemia and reflow in rat renal proximal tubule epithelia cells. In addition, the preventive effects of inosine and Rhizoma Ligustici Chuanxiong on ischemia and reperfusion cell injury were studied. The results showed that the preventive effects of inosine and Rhizoma Ligustici Chuanxiong were obvious. Redistribution and slight increase of calcium occurred during ischemic period in the proximal tubule cells. In this period the changes of plasma membrane permeability were gentle. Following the extention of reflow time, a large amount of calcium deposited in the cells and mitochondria. The plasma membrane became broken.