ABSTRACT
ObjectiveTo investigate the effects of morin treatment on bone metabolism and bone mass in aged rats， and to clarify the possible mechanism. MethodsTen young female Sprague-Dawley rats （3 months old） and 20 old female Sprague-Dawley rats （24 months old） were randomly divided into three groups： Control group （CON， 10 young rats）； Model group （MOD， 10 young rats）； 10 old rats and SangHuangSu Group （SSS， 10 old rats）. During the experiment， the SSS group received intraperitoneal injection of morin （10 mg / kg） daily. The treatment lasted for 12 weeks. After treatment， Micro-CT， HE stained sections， serological tests and Western blot were used to observe the treatment effect and possible mechanism. ResultsAfter 12 weeks of treatment， compared with MOD group， the number and density of bone trabeculae in SSS group were significantly improved. The BMD， Conn. D， Tb. N， Tb.Th and Tb.Sp of the left femur in the SSS group were significantly better than those in the MOD group（P <0.05）. After 12 weeks of treatment， the levels of CTX-1， osteocalcin， TRACP-5b and PINP in SSS group were significantly lower than those in MOD group（P <0.05）. Compared with the MOD group， the ERK1/2-p38 signal pathway was significantly inhibited and the levels of ERK1/2 and p38 were significantly decreased in the SSS group（P <0.05）. ConclusionMorin pigment mediates the protective effect on the bones of aged rats by inhibiting the ERK1/2-p38 signaling pathway and reducing bone turnover.
ABSTRACT
METHODS: Rats received a hibateral intracerebroventricular fusion of Aβ25-35. Tetramethylpyrazine was administrated through i.v. injection at 20 and 60 mg��kg-1��d-1 for 21 d. The expression of RAGE and inflammatory factors(including IL-1β, IL-6, TNF-α)were assessed immunohistochemical and ELISA, respectively. Further, an AD cell model (primary cultured hippocampal neurons of SD neonatal rats) and a RAGE-over expressing cell model(primary cultured hippocampal neurons transfected with pCMV/hygro-RAGE) were used for investigating the mechanisms of tetramethylpyrazine's effects. Cell viability was determined by MTT assay and intracellular reactive oxygen species (ROS) was identified by DCFH-DA staining. Real time fluorescent quantitative PCR and Western blot method were employed to detect the transcriptional level(RAGE)and phosphorylation of targeted proteins (including ERK1/2, p38, IκB) in all groups.