The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)

The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the three populations of SP (Fst > 0.288); genetic dissimilarity was related to the geographical distance. The ISSR proved to be efficient and useful molecular markers in comparison with other markers most widely used for preliminary diagnosis of genetic diversity in populations of amphibians, and could be applied as a tool for future conservation projects, since they could identify potential ESUs and influence decisions on the preservation of fragments.

The Studies of Modified Doos SACSIH Operation , Electrode for Resection of Endocervical Canal , and Hysterectomy Scoring System / 대한산부인과학회잡지

The methods of hysterectomy for intrapelvic benign disease include total, subtotal, la-paroscopic-assisted transvaginal, and SACSIH operation. There have been 298 cases of SACSIH operation for benign uterine or pelvic diseases at the Chonbuk National University Hospital from November 16, 1992 to March 31, 1997. Patients were 41.8 years old on the average ranging from 29 to 50. Their indications cover uterine myoma(211), adenomyosis(37), uterine myoma with pelvic adhesion(16), uterine my- oma with endometriosis(11), and others(23), the uterine myoma being the most common. Both CIN and CIS is excluded after the full evaluation of the cervical disease through Pap. smear, cervicography, ECC biopsy, and HPV typing etc, and the consent of patient is secu- red. The modified SACSIH operation, when compared with other simple hysterectomy oper- ation, has the following advantages;1) minimal surgery, 2) easy adaptation, 3) avoidance of stump cancer, 4) rapid recovery and 5) less postoperative care as far as psychosomatic posthysterectomy problems are concerned. One of the new equipment for new modified technique is the Electro-Surgical Unit system with Electrode for endocervical canal resection. This is developed at this hospital as a part of the G-7 project supported by the Ministry of Health and Welfare. It is made of electrode and rod from electrosurgical unit system and operated by high frequency of current. Thus, we introduce, herein, the modified SACSIH operation and propose the postopera- tional scoring system to estimate postoperative sequelae and to help carry on personal well- being in terms of sexuality and normal life.

Research and realization of ECG monitoring system against high-frequency interference by electrosurgical unit / 医疗卫生装备

In modern surgeries, ESU is an important electrosurgical equipment. However, the interference caused by ESU usually affects real-time monitoring of the patient. To solve these problems, the paper introduces some new ideas in analog circuits. To the problem of interference and base-line excursion, this paper proposes an adaptive comb filter with Phase-Locked Loop(PLL), besides digital comb filters. Following the frequency of power and sampling accordingly, this method could easily clear the interference, which could make the cost reduced.