ABSTRACT
Objective: To develop the EST-SSR molecular identification system of Lilium lancifolium, Lilium davidii var. willmottiae, Lilium regale, Lilium casa blanca and Lilium brownie var. viridulum, and analyze the development efficiency and identification ability of EST-SSR molecular marker technology for Lilium genus. Methods: The MISA.pl program was used to identify the SSR locus of the EST gene sequence published by NCBI. The EST-SSR primers were generated by Primer3 program module, and the primers were screened by PCR amplification and agarose gel electrophoresis. The primary screening primers were verified by polyacrylamide gel electrophoresis, and the characteristic bands of different germplasms were labeled and analyzed to construct an identification system. Results: A total of 199 pairs of SSR primers were designed. After screening 26 pairs of primers, two pairs of highly efficient primers were obtained. The molecular identification system constructed by primer JZ391 can effectively identify the mixed commercial materials, which had certain practical value. Conclusion: Based on the extreme genetic characteristics of the research materials, this identification marker development is very efficient. The results confirm that the genetic basis of the species is an important factor affecting the development efficiency of its EST-SSR molecular marker. At the same time, this case can be used as a reference for the development of EST-SSR markers for other Chinese medicinal herbs similar to Lilium genus.
ABSTRACT
AIM: To compare the difference between medicinal and edible lilium brownii. METHODS: GC method was used for the determination of stigmasterol (the reference standard) in medicinal and edible Lilium brownii. And pharmacology experiment on stigmasterol was carried out. RESULTS: Stigmasterol had the antiinflammatory and anti-coughing effect. It represented the main effect of Lilium brownii. It was reasonable to be the reference standard. There were obvious differences in the stigmasterol content of medicinal and edible Lilium brownii. The stigmasterol content of medicinal Lilium brownii was much higher than that of the edible. CONCLUSION: Edible Lilium brownii can not be applied to clinical.