ABSTRACT
Consistency in quality of traditional Chinese medicine granules is an important factor to ensure reproducible clinical efficacy. In this study rhubarb dispensing granules were utilized to construct an efficacious near-infrared spectroscopy (eNIRS) assay by combining NIRS and biopotency. A NIR method for assaying rhubarb dispensing particles was established, and information on different batches was collected. The diarrhea-inducing biopotency of rhubarb dispensing granules was determined based on a constipation model induced by diphenoxylate in mice. The animal protocol was approved by the Animal Ethic Committee of 302 Hospital of Chinese PLA People's Liberation Army (ID: IACUC-2019-0010). Ten anthraquinones were determined in rhubarb dispensing granules by UPLC. The correlation between NIR and biopotency was analyzed and five characteristic bands that correlated highly with bioactivity were identified, including 4 011-4 390, 4 859-5 461, 7 012-7 493, 10 992-11 312 and 11 871-12 489 cm-1. There were some differences in the main bands of different chemical constituents. In summary, five active bands based on NIRS were identified and found to be able to achieve rapid on-line detection of rhubarb dispensing granule quality. This research model may also provide reference for quality control of other Chinese medicine dispensing granules.
ABSTRACT
Background: Infectious diarrheal disease is the most frequent reason of diarrhea around the world and it is the leading cause of death amongst children. Vancomycin has been regarded as the drug of choice for management of C. difficile-related colitis. However, outbreaks of infection with vancomycin resistant enterococci have led to restriction in its use. The present study was conducted with the aim to determine the efficacy of different antibiotics in managing diarrhea cases. Materials and methods: The study was conducted in the Department of medicine for a period of 8 months. All the subjects were randomized into three groups, receiving metronidazole, vancomycin and teicoplanin respectively. No additional drugs or probiotics were administered to the patients so that the exacting efficacy of the drug can be established. Both clinical and laboratory evaluations were used to assess and monitor the adverse events. Chi square test and student t test were used to analyze the differences between the groups. Probability value of less than 0.05 was considered significant. Results: The study included a total of 90 subjects. There were 30 subjects who received metronidazole, 32 subjects received vancomycin and 28 subjects received Teicoplanin. The mean age of the subjects was 46 +/- 8 years. The mean temperature in all the three groups was 36.8+/-0.8, 39.1+/-0.3 and 37.2+/-0.4 respectively. Clinical cure was seen amongst 93.3% subjects receiving metronidazole, 93.7% subjects receiving vancomycin and 96.4% subjects receiving teicoplanin. There was no significant difference between the groups as the p value was more than 0.05. Conclusion: From the present study, all the three drugs have been found equally efficacious in managing cases of diarrhea.
ABSTRACT
Fingerprint of Chinese materia medica (CMM), which could be used to comprehensively analyze the information of the type and content distribution of the effective and ineffective components in CMM, is in line with the integral and fuzzy characteristics of CMM and has held its own important space in modern and future CMM quality control. As more and more technologies have been applied to the fingerprint research of CMM, it is sure to play a much more important role in many aspects, such as the quality control of CMM, the researches of efficient components, and the mechanism in CMM, and so on. This paper generalizes the research in progresses in research and analytic methods on CMM fingerprint technology, and the prospect of the future development in the fingerprint is discussed.
ABSTRACT
Objective To research on the new technique and method of bone grafting for nonunion of four limbs. Methods A new method of bone grafting was developed to take advantage of Nitinol Shape Memory Connector. It included splinting mode, padding mode and seeding mode. 148 cases of bone nonunion in 134 patients were treated with the modified method. Results The patients were followed up for 5.5 months to 6 years, with an average period of 2.25 years. On the average, the nonunion healed with lamellar bone 3.5 months after the operation. The healing rate was 99.3% . Conclusion The author s modified bone grafting provides a new and efficacious method for treatment of the limb nonunion.