ABSTRACT
This study assessed the impact of the electrochemically activated aqueous solutions (ECA AS) on the growth and formationof the biofilm of lactic acid bacteria (LAB). The effectiveness of biocides produced by the unipolar electrochemicalactivation of aqueous solutions of sodium chloride was studied. It was found that sequential treatment of the biofilm withcatholyte and anolyte (fractions ECA AS) lead to the most pronounced decrease in the growth rate and density of bacteria.The results obtained demonstrate antibacterial efficacy and the possibility of using ECA AS for the prevention anddisinfection of aquatic systems, for example, at the enterprises of the agro-industry and the food industry
ABSTRACT
Objective: To establish an ultra-performance liquid chromatography(UPLC)method for the simul-taneous determination of four sesquiterpene lactones, deoxyelephantopin, isodeoxyelephantopin, isoscabertopin and sca- bertopin in Elephantopus scaber Linn.. Methods: The UPLC conditions were as follows: the separation was performed using an Acquity UPLC BEH C18(2.1 mm × 100 mm, 1.7 μm)column, the temperature of the column was maintained at 40℃, and the detection wavelength was 220 nm. The mobile phase was the water and acetonitrile solution in a binary gra- dient elution at the flow rate of 0.3 ml/min. Results: The four components, deoxyelephantopin, isodeoxyelephantopin, isoscabertopin and scabertopin were separated well under the UPLC conditions and showed a good linearity within the ranges of 0.383-76.500(R2=0.9999), 0.775-155.0(R2=0.9999), 0.507-101.5(R2=0.9999), and 0.850-(R2=0.9997), respectively. Their average recovery(n=6)was 102.31%, 103.87%, 102.66% and 105.68%, RSD was 1.20%, 2.00%, 1.24% and 1.73%, respectively. The four components were validated by the simultaneous termination in eleven batches of E. scaber samples from different areas in the present study. Conclusion: lished UPLC method could be used for the determination of four sesquiterpene lactones, deoxyelephantopin, elephantopin, isoscabertopin and scabertopin in E. scaber. The present results provide a reference for further velopment and quality evaluation of E. scaber.